Nematicidal activity of malabaricones isolated from Myristica malabarica fruit rinds against Bursaphelenchus xylophilus

Abstract

Plant extracts from 50 plant species were tested for their nematicidal activities against Bursaphelenchus xylophilus, the causative agent of pine wilt disease. Out of 50 extracts, the methanol extract of Myristica malabarica fruit rinds showed the most potent nematicidal activity with mortality of 100% at a concentration of 1000 μg ml −1 against B. xylophilus. Three nematicidal substances were isolated by bioassay-guided fractionation and then identified as malabaricones A, B and C by mass and NMR spectral data. Both malabaricones B and C showed higher activity than malabaricone A. There was a significant synergistic interaction between the three compounds on the pine wood nematode. When the combination of three compounds at a ratio of 1 : 1 : 1 was applied, its EC 50 value was 11.8 μg ml −1 . These results indicate that the extract of M. malabarica containing the three resorcinols could be useful as a natural nematicide for the control of pine wilt disease.

Nematicidal and Antifungal Activities of Annonaceous Acetogenins from Annona squamosa against Various Plant Pathogens

“…The nematicidal activity of test materials against PWN was assessed by the microtiter plate assay method as previously described by Choi et al 12 PWN was grown on the mycelia of B. cinerea in a PDA Petri dish for 7 days at 28 °C. The J2s of PWN were collected with distilled water containing 0.01% (v/v) Triton X-100.…”

Nematotoxic coumarins from Angelica pubescens Maxim. f. biserrata Shan et Yuan roots and their physiological effects on Bursaphelenchus xylophilus

“…Phytonematicides as natural products are considered popular candidates. Many plants have been reported to provide potential nematotoxic constituents including essential oils, alkaloids, and other constituents (Matsuda et al, 1989(Matsuda et al, , 1991Zhao, 1999;Choi et al, 2006Choi et al, , 2007Choi et al, , 2008; Kong et al, 2006Kong et al, , 2007Andrrk et al, 2007;Kim et al, 2008;Wang et al, 2008;Faria et al, 2010Faria et al, , 2013Zhang et al, 2011;Wang et al, 2012;Ntalli and Caboni, 2012;Cui et al, 2014;Guo et al, 2016Guo et al, , 2017. The plant Angelica pubescens Maxim.…”

Current Status of Botanical Pesticides for Crop Protection

“…Three diarylnonanoids malabaricones A, B and C (Fig. 3) from Myristica malabarica caused mortalities from 31-75% for PWN (Choi et al, 2008). Methyl pelargonate (Fig.…”