Nematicidal Activity of Chaetoglobosin A Poduced by <i>Chaetomium globosum</i> NK102 against Meloidogyne incognita

Hu, Yang; Zhang, Weipu; Zhang, Ping; Ruan, Weibin; Zhu, Xudong

doi:10.1021/jf304314g

Cited by 95 publications

(71 citation statements)

References 30 publications

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“…This might explain our observation of the effect of chaetoglobosin A and its derivative 19- O -acetylchaetoglobosin A on paralyzing the tested nematodes at 50 and 100 μg/mL. However, at higher concentrations (300 μg/mL) chaetoglobosin A was reported to have toxic effects and caused nematode mortality [26]. Thus, chaetoglobosins produced by I .…”

Section: Discussionmentioning

confidence: 90%

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Ijuhya vitellina sp. nov., a novel source for chaetoglobosin A, is a destructive parasite of the cereal cyst nematode Heterodera filipjevi

et al. 2017

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Cyst nematodes are globally important pathogens in agriculture. Their sedentary lifestyle and long-term association with the roots of host plants render cyst nematodes especially good targets for attack by parasitic fungi. In this context fungi were specifically isolated from nematode eggs of the cereal cyst nematode Heterodera filipjevi. Here, Ijuhya vitellina (Ascomycota, Hypocreales, Bionectriaceae), encountered in wheat fields in Turkey, is newly described on the basis of phylogenetic analyses, morphological characters and life-style related inferences. The species destructively parasitises eggs inside cysts of H. filipjevi. The parasitism was reproduced in in vitro studies. Infected eggs were found to harbour microsclerotia produced by I. vitellina that resemble long-term survival structures also known from other ascomycetes. Microsclerotia were also formed by this species in pure cultures obtained from both, solitarily isolated infected eggs obtained from fields and artificially infected eggs. Hyphae penetrating the eggshell colonised the interior of eggs and became transformed into multicellular, chlamydospore-like structures that developed into microsclerotia. When isolated on artificial media, microsclerotia germinated to produce multiple emerging hyphae. The specific nature of morphological structures produced by I. vitellina inside nematode eggs is interpreted as a unique mode of interaction allowing long-term survival of the fungus inside nematode cysts that are known to survive periods of drought or other harsh environmental conditions. Generic classification of the new species is based on molecular phylogenetic inferences using five different gene regions. I. vitellina is the only species of the genus known to parasitise nematodes and produce microsclerotia. Metabolomic analyses revealed that within the Ijuhya species studied here, only I. vitellina produces chaetoglobosin A and its derivate 19-O-acetylchaetoglobosin A. Nematicidal and nematode-inhibiting activities of these compounds have been demonstrated suggesting that the production of these compounds may represent an adaptation to nematode parasitism.

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Section: Discussionmentioning

confidence: 90%

“…Therefore, environmentally friendly and biologically effective alternatives for the control of nematode plant pests are urgently needed. They may consist in the application of whole organisms or in biologically active fungal compounds only [12, 13, 22–26]. …”

Section: Introductionmentioning

confidence: 99%

Ijuhya vitellina sp. nov., a novel source for chaetoglobosin A, is a destructive parasite of the cereal cyst nematode Heterodera filipjevi

et al. 2017

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“…According to previous studies, methanol, 21 ethyl acetate, 22 acetone, dichloromethane, 23 chloroform, 4 and n-hexane 24 were chosen to determine the best solvent for extraction of chaetoglobosin A. In addition, ethanol was also tested together with other solvents owing to its low cost and the fact that it is a primary alcohol (like methanol).…”

Section: Resultsmentioning

confidence: 99%

“…Chaetoglobosin A, which is biosynthesized mainly by Chaetomium globosum , is the most abundant member of this family 3 and displays various biological activities. Chaetoglobosin A shows highly toxic effects against human cancer cell lines, a murine leukemia cell line, and Caenorhabditis elegans ,3, 4, 5 and also shows phytotoxicity against alfalfa seedlings 6 as well as acute toxic effects against various types of microorganisms such as Setosphaeria turcica , Rhizopus stolonifer , and Coniothyrium diplodiella 7, 8, 9. Despite these broad effects, development of an effective and economically feasible chaetoglobosin A production method remains challenging due to the low output by microorganisms and the high cost of the required culture substrates and purification process.…”

Section: Introductionmentioning

confidence: 99%

New production process of the antifungal chaetoglobosin A using cornstalks

Jiang

Zhang

et al. 2017

Brazilian Journal of Microbiology

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Chaetoglobosin A is an antibacterial compound produced by Chaetomium globosum, with potential application as a biopesticide and cancer treatment drug. The aim of this study was to evaluate the feasibility of utilizing cornstalks to produce chaetoglobosin A by C. globosum W7 in solid-batch fermentation and to determine an optimal method for purification of the products. The output of chaetoglobosin A from the cornstalks was 0.34 mg/g, and its content in the crude extract was 4.80%. Purification conditions were optimized to increase the content of chaetoglobosin A in the crude extract, including the extract solvent, temperature, and pH value. The optimum process conditions were found to be acetone as the extractant, under room temperature, and at a pH value of 13. Under these conditions, a production process of the antifungal chaetoglobosin A was established, and the content reached 19.17%. Through further verification, cornstalks could replace crops for the production of chaetoglobosin A using this new production process. Moreover, the purified products showed great inhibition against Rhizoctonia solani, with chaetoglobosin A confirmed as the main effective constituent (IC50 = 3.88 μg/mL). Collectively, these results demonstrate the feasibility of using cornstalks to synthesize chaetoglobosin A and that the production process established in this study was effective.

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“…Among substitutive chemicals, currently registered alternatives to MB are chloropicrin (CP); 1,3-dichloropropene (1,3-D); methyl isothiocyanate (MITC) generators such as Metam sodium (MNa) and Dazomet (DZ), abamectin (AB) and their combinations (Qiao et al, 2012;Mao et al, 2012;Yan et al, 2012). Other alternatives to MB fumigation may be the use of non-chemical methods, such as soil solarization, organic amendments and biocontrol agents (Klein et al, 2011;Caboni et al, 2013;Hu et al, 2013). However, nonchemical control methods alone are often unsuitable because they do not provide the broad-spectrum activity or the degree of consistency achieved with MB fumigation (Chellemi, 2002).…”

Section: Introductionmentioning

confidence: 99%