Nematicidal activity of a water soluble lectin from seeds of Moringa oleifera

Medeiros, Mário Luan Silva de; Moura, Márcio das Chagas; Napoleão, Thiago Henrique; Paiva, Patrícia Maria Guedes; Bezerra, Ana Carla Diógenes Suassuna; Silva, Michele Dalvina Correia da

doi:10.1016/j.ijbiomac.2017.10.167

Cited by 24 publications

(9 citation statements)

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“…Erica cinera was more active against eggs and L 3 of T. columbriformis than to inhibit the egg hatching and larvae exsheathment of abomasal parasites ( H. contortus and T. circumcinta ) (Moreno-Gonzalo et al ., 2013 a , 2013 b ). The Agave sisalana and Moringa oleifera has greater ovicidal effect while Acacia nilotica and A. raddiana are more active against L 3 of GIN (Botura et al ., 2013; Zabré et al ., 2017; De Medeiros et al ., 2018). These variations can be related with differences in various enzymatic constituents and membrane structures of the species and nematodes' life stages.…”

Section: Discussionmentioning

confidence: 99%

“…De Medeiros et al . (2018) evaluated the in vitro effect of water-soluble Moringa oleifera lectin (WSMoL) on hatching of eggs and on the development of early-stage larvae of gastrointestinal nematodes from naturally infected goats. The mechanism of action of this substance is attributed to the interference of WSMoL on the activity of proteases and the affinity of the lectin for glycosylated, interacting with intestinal glycoconjugate receptors in the embryo, as well as in cuticle of the larvae.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the investigation of the antiparasitic activity of natural bioproducts can contribute to the development of alternative treatments and the reduction of the dependence on conventional chemotherapy (Santos et al ., 2017). The interest in their anti-parasitic properties is increasing as illustrated by several reviews concerning ethnoveterinary medicine in different continents (Hounzangbe-Adote et al ., 2005; De Medeiros et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

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Anthelmintic activity of plants against gastrointestinal nematodes of goats: a review

et al. 2019

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The gastrointestinal nematodes (GIN) stand out as an important cause of disease in small ruminant, especially on goat farm. Widespread resistance to synthetic anthelminthics has stimulated the research for alternative strategies of parasite control, including the use of medicinal plants. The present work summarizes the in vitro and in vivo studies of plants with activity against GIN of goats, focusing on the description of chemical constituents related to this effect. This review retrieved 56 scientific articles from 2008 to 2018 describing more than 100 different plant species. The most frequently investigated family was Fabaceae (30.7%). Most in vitro studies on the activity of plant extracts and fractions were carried out with of free-living stages nematodes. In vivo studies were conducted mainly with the use of plants in animal feed and generally showed lower effectiveness compared to in vitro assays. The main plant secondary metabolites associated with anthelmintic effect are condensed tannins, saponin and flavonoids. However, the studies with compounds isolated from plants and elucidation of their mechanisms of action are scarce. Herbal medicines are thought to be promising sources for the development of effective anthelmintic agents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anthelmintic activity of plants against gastrointestinal nematodes of goats: a review

et al. 2019

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“…Medeiros et al . reported the ovicidal activity of WSMoL against gastrointestinal parasites from goats and suggested that this was due to lectin interaction with chitin.…”

Section: Discussionmentioning

confidence: 99%

Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs

Alves

Soares

Bento

et al. 2019

Pest Management Science

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BACKGROUND: Lectins from Moringa oleifera seeds (WSMoL), Myracrodruon urundeuva bark (MuBL), and heartwood (MuHL) are larvicidal agents against Aedes aegypti; in addition, WSMoL is an ovicidal agent against this mosquito. In this work, we evaluated the ovicidal activity of MuBL and MuHL by determining the concentrations that reduce the hatching rates by 50% in 72 h (EC 50 ). The effects of WSMoL, MuBL, and MuHL on the ultrastructure of the eggs' surface were assessed by scanning electron microscopy (SEM). In addition, the ability of these lectins to penetrate the eggs was investigated by using conjugates of the lectins with fluorescein isothiocyanate (FITC). RESULTS: MuBL andMuHL were ovicidal agents with EC 50 of 0.26 and 0.80 mg/mL (260 and 800 ppm), respectively. SEM images of eggs treated with WSMoL for 24 h revealed discontinuity of the exochorionic network and the absence of the exochorionic cells and their tubercles. After 48 and 72 h of incubation, strong deformation and degeneration of egg surfaces were observed. In MuBL and MuHL-treated eggs, the presence of lumps on the surface of the eggs, disappearance of the exochorionic network and the decrease and deformation of tubercles were observed. Lastly, fluorescence microscopy revealed that the three lectins were able to enter the eggs and reach the digestive tract of the embryos. CONCLUSION: WSMoL, MuBL, and MuHL are ovicidal agents on A. aegypti that have differing efficiencies in terms of how they cause alterations in the chorionic surface and in terms of their ability to penetrate the eggs.

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“…13 Among the components present in Moringa oleifera seed, water-soluble lectin (WSMoL) has the property of Aedes aegypti larvicide and ovicide 14 and anti nematodes. 15 As all scientific knowledge about Moringa oleifera is still based on experimental studies, there is a need to respect the stages of clinical research for use in humans. Thus, in 2019 in Brazil, the National Health Surveillance Agency (ANVISA) prohibited the manufacture, import, marketing, advertising and distribution of all foods containing Moringa oleifera (RESOLUTION-RE No.…”

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confidence: 99%