Nematicidal Activity of a Nonpathogenic Biocontrol Bacterium, Pseudomonas chlororaphis O6

Lee, Jang Hoon; Cheol, Kyung; Ko, Sug Ju; Kang, Beom Ryong; Kim, In Soo; Kim, Young Cheol

doi:10.1007/s00284-010-9779-y

Cited by 45 publications

(33 citation statements)

References 20 publications

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“…strain ZE36 on nematodes was not directly tested. Work with Pseudomonas chlororaphis O6 implicated hydrogen cyanide in the suppression of the root-knot nematode Meloidogyne hapla by noting the glycine-dependent nature of the inhibition (Lee et al 2011). Glycine is a precursor to cyanide formation (Wissing 1974).…”

Section: Invertebrates Influenced By Cyanide Of Bacterial Originmentioning

confidence: 99%

Bacterial cyanogenesis: impact on biotic interactions

Zdor

2014

J Appl Microbiol

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SummaryThe ability of bacteria to influence organisms that they associate with via metabolite production is one of the hallmarks of microbial interactions. One metabolite of interest is the metabolic poison cyanide. Production of this metabolite is an unique characteristic of certain bacteria that inhabit a wide array of habitats ranging from the human body to the rhizosphere. This review focuses on four targets of cyanogenic bacteria: the human lung, plant pathogens, plants and invertebrates. For a number of cyanogenic bacteria, the contribution of cyanide to the interaction has been rigorously tested using mutants altered in cyanide production. Both deleterious and stimulatory effects of cyanogenic bacteria on other organisms have been documented. In addition, the HCN synthase-encoding gene cluster hcnABC has served as a marker of cyanogenic capability in the soil environment revealing both genetic diversity at this locus and regulatory influences by other organisms. The pervasive nature of cyanogenesis in a number of different ecological contexts encourages exploration of this bacterial ability and its possible optimization for improving human health, crop production and pest control.

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Section: Invertebrates Influenced By Cyanide Of Bacterial Originmentioning

confidence: 99%

Bacterial cyanogenesis: impact on biotic interactions

Zdor

2014

J Appl Microbiol

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“…In pathogens, the Gac sensor kinase system controls virulence gene expression (Heeb and Haas, 2001). It is also found that the Gac/Rsm system of PcO6 is required for the expression of the insecticidal activity to the tobacco hornworm (Rangel et al, 2016), and to the root knot nematode (Lee et al, 2011(Lee et al, , 2017. In addition, the Gac system is shown to be important in expression of gene expression in the inhibition of fungal growth by PcO6 Oh et al, 2013a;Park et al, 2011).…”

Section: Gac/rsm System a Master Switch For Global Regulationmentioning

confidence: 99%

“…Hosts other than plants also are affected by colonization with PcO6, because this bacterium possessed insecticidal capacity. This bacterium showed insecticidal activity on the tobacco hornworm (Rangel et al, 2016) and on nematodes (Lee et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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The Gac/Rsm Signaling Pathway of a Biocontrol Bacterium, Pseudomonas chlororaphis O6

Anderson

Kang

Kim

2017

Research in Plant Disease

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Pseudomonas chlororaphis O6, isolated from the roots of dryland, field-grown commercial wheat in the USA, enhances plant health and therefore it is used in agriculture as a biofertilizer and biocontrol agent. The metabolites produced by this pseudomonad stimulate plant growth through direct antagonism of pathogens and by inducing systemic resistance in the plant. Studies upon P. chlororaphis O6 identify the pathways through which defined bacterial metabolites generate protection against pathogenic microbes, insects, and nematodes. P. chlororaphis O6 also triggers plant resistance to drought and salinity stresses. The beneficial determinants are produced from bacterial cells as they form biofilms during root colonization. Molecular control these processes in P. chlororaphis O6 involves the global regulatory Gac/Rsm signaling cascade with cross-talk between other global regulatory pathways. The Gac/Rsm regulon allows for coordinate phasing of expression of the genes that encode these beneficial traits among a community of cells. This review provides insights on the Gac/Rsm regulon in expression of beneficial traits of the P. chlororaphis O6 which can contribute to help yield enhancement and quality in agricultural production.

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“…The antifungal agents include hydrogen cyanide (HCN), siderophores, biosurfactants and antibiotics, and enzymes including proteases, lipases, chitinases, and glucanases (Dubis et al, 2007; Haas and Defago, 2005; Raaijmakers et al, 2002). The root colonizer Pseudomonas chlororaphis O6 produces several compounds with antifungal activity including a pyoverdine-like siderophore, phenazines, pyrrolnitrin and HCN (Kang et al, 2007; Lee et al, 2011; Park et al, 2011). Effective root colonization by P. chlororaphis O6 also protects plants from pathogens through induction of systemic resistance against various plant diseases as well as drought and salinity stress (Cho et al, 2008; 2012; Han et al, 2006).…”

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confidence: 99%

Proteomic Analysis of a Global Regulator GacS Sensor Kinase in the Rhizobacterium, Pseudomonas chlororaphis O6

Kim

Anderson

et al. 2014

The Plant Pathology Journal

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The GacS/GacA system in the root colonizer Pseudomonas chlororaphis O6 is a key regulator of many traits relevant to the biocontrol function of this bacterium. Proteomic analysis revealed 12 proteins were down-regulated in a gacS mutant of P. chlororaphis O6. These GacS-regulated proteins functioned in combating oxidative stress, cell signaling, biosynthesis of secondary metabolism, and secretion. The extent of regulation was shown by real-time RT-PCR to vary between the genes. Mutants of P. chlororaphis O6 were generated in two GacS-regulated genes, trpE, encoding a protein involved in tryptophan synthesis, and prnA, required for conversion of tryptophan to the antimicrobial compound, pyrrolitrin. Failure of the trpE mutant to induce systemic resistance in tobacco against a foliar pathogen causing soft rot, Pectobacterium carotovorum SCCI, correlated with reduced colonization of root surfaces implying an inadequate supply of tryptophan to support growth. Although colonization was not affected by mutation in the prnA gene, induction of systemic resistance was reduced, suggesting that pyrrolnitrin was an activator of plant resistance as well as an antifungal agent. Study of mutants in the other GacS-regulated proteins will indicate further the features required for biocontrol-activity in this rhizobacterium.

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Nematicidal Activity of a Nonpathogenic Biocontrol Bacterium, Pseudomonas chlororaphis O6

Cited by 45 publications

References 20 publications

Bacterial cyanogenesis: impact on biotic interactions

Bacterial cyanogenesis: impact on biotic interactions

The Gac/Rsm Signaling Pathway of a Biocontrol Bacterium, Pseudomonas chlororaphis O6

Proteomic Analysis of a Global Regulator GacS Sensor Kinase in the Rhizobacterium, Pseudomonas chlororaphis O6

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