Nemafric-BL phytonematicide induces egg hatch inhibition in<i>Meloidogyne incognita</i>

Dube, Z. P.; Mashela, P. W.

doi:10.1080/09064710.2016.1155641

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Nevertheless, the triterpenic cucurbitacins and/or the non-proteinaceous amino acid cucurbitin may be accountable for the nematicidal properties of mCOPE. While these respective components are predominantly and uniquely found in Cucurbitaceae plants and fruits (Miró, 1995;Chen et al, 2005;Wang et al, 2014), both have been suggested to be the responsible antihelmintic agent in various cucurbit extracts (Marie-Magdeleine et al, 2009;Dube and Mashela, 2016;Grzybek et al, 2016;Mashela and Shokoohi, 2021). With respect to the IR-triggering moiety, the range of tentative candidates is wide.…”

Section: Discussionmentioning

confidence: 99%

Cucurbitaceae COld Peeling Extracts (CCOPEs) Protect Plants From Root-Knot Nematode Infections Through Induced Resistance and Nematicidal Effects

et al. 2022

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With nematicides progressively being banned due to their environmental impact, an urgent need for novel and sustainable control strategies has arisen. Stimulation of plant immunity, a phenomenon referred to as “induced resistance” (IR), is a promising option. In this study, Cucurbitaceae COld Peeling Extracts (CCOPEs) were shown to protect rice (Oryza sativa) and tomato (Solanum lycopersicum) against the root-knot nematodes Meloidogyne graminicola and Meloidogyne incognita, respectively. Focusing on CCOPE derived from peels of melon (Cucumis melo var. cantalupensis; mCOPE), we unveiled that this extract combines an IR-triggering capacity with direct nematicidal effects. Under lab conditions, the observed resistance was comparable to the protection obtained by commercially available IR stimuli or nematicides. Via mRNA sequencing and confirmatory biochemical assays, it was proven that mCOPE-IR in rice is associated with systemic effects on ethylene accumulation, reactive oxygen species (ROS) metabolism and cell wall-related modifications. While no negative trade-offs were detected with respect to plant growth or plant susceptibility to necrotrophic pests or pathogens, additional infection experiments indicated that mCOPE may have a predominant activity toward biotrophs. In summary, the presented data illustrate a propitious potential for these extracts, which can be derived from agro-industrial waste streams.

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Section: Discussionmentioning

confidence: 99%

Cucurbitaceae COld Peeling Extracts (CCOPEs) Protect Plants From Root-Knot Nematode Infections Through Induced Resistance and Nematicidal Effects

et al. 2022

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“…J2 that hatched during the first 24 h were discarded. J2 that hatched during the subsequent 48 h were used in the bio-assay (Dube and Mashela 2016).…”

Section: Collection Of Meloidogyne Incognita Eggs and Secondstage Juveniles (J2)mentioning

confidence: 99%

Sensitivity of Meloidogyne incognita second-stage juvenile hatch, motility and viability to pure cucurbitacins and cucurbitacin-containing phytonematicides

Dube

Mashela

Waele

2018

South African Journal of Plant and Soil

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Informa UK Limited (trading as Taylor & Francis Group) and NISC (Pty) Ltd This is the final version of the article that is published ahead of the print and online issue During the past decade, efforts to develop botanicals as biopesticides have increased (Okwute 2012). As a result, a number of promising biopesticides have been identified (Manju and Sankari Meena 2015). Nonetheless, many issues remain to be solved before these biopesticides can be effectively applied. One of these issues is the claim that the purified active ingredients from botanicals are less effective compared with the fermented crudeextracted biopesticides (Javed et al. 2008;Okwute 2012;Chaudhary et al. 2013). For instance, the Aza formulation, which contains azadirachtin, a purified ingredient from neem seed, showed no effect on the motility and viability, and a lower effect on second-stage juveniles (J2) hatch of root-knot nematode (Meloidogyne species) compared with crude neem cake and leaf extracts (Javed et al. 2008). Grandison (1992 cited by Javed et al. 2008) also did not observe any direct effects of purified substances from neem seed kernels, such as aza, salanine and nimbin, on J2 of Meloidogyne javanica.The first step in determining the bioactivity of plant extracts in most studies consists of in vitro bioassays of purified substances. The inactivity of some of the purified substances resulted in potential phytonematicides failing to move beyond in vitro bioassays, limiting progress in the development of phytonematicides. Okwute (2012) suggested two causes for the observed inactivity of purified substances:(1) less desirable solvents used during purification and

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Nemafric-BL phytonematicide induces egg hatch inhibition inMeloidogyne incognita

Cited by 2 publications

References 5 publications

Cucurbitaceae COld Peeling Extracts (CCOPEs) Protect Plants From Root-Knot Nematode Infections Through Induced Resistance and Nematicidal Effects

Cucurbitaceae COld Peeling Extracts (CCOPEs) Protect Plants From Root-Knot Nematode Infections Through Induced Resistance and Nematicidal Effects

Sensitivity of Meloidogyne incognita second-stage juvenile hatch, motility and viability to pure cucurbitacins and cucurbitacin-containing phytonematicides

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