Nelumbo nucifera Gaertn.

Tang, Weici; Eisenbrand, Gerhard

doi:10.1007/978-3-642-73739-8_89

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Nelumbo nucifera (lotus, belonging to the family of Nelumbonaceae) has long been cultivated in East Asia and extensively used as a traditional medicinal plant as well as health food in China . The leaves are recognized as the medicinal part of N. nucifera , as mentioned in the Chinese Pharmacopoeia .…”

Section: Introductionmentioning

confidence: 99%

Lipase Ligands in Nelumbo nucifera Leaves and Study of Their Binding Mechanism

Zhu

Jia

Liu

et al. 2014

J. Agric. Food Chem.

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Lotus (Nelumbo nucifera) leaves have been widely used in weight-loss foods to prevent obesity in China. In this work, a facile procedure based on ligand fishing was developed to isolate and identify lipase inhibitors present in lotus leaves. Highly stable and active lipase-Fe3O4 superparamagnetic nanoparticle conjugates (LMNPs) were prepared and used as baits. Two flavonoids in lotus leaf extract were found to bind to the baits and were identified as quercetin-3-O-β-d-arabinopyranosyl-(1→2)- β-d-galactopyranoside (1) and quercetin-3-O-β-d-glucuronide (4) based on electrospray ionization-mass spectrometric analyses. Their 50% inhibitory concentrations on lipase (IC50) were 52.9 ± 3.2 and 17.1 ± 1.5 μg/mL, respectively. In addition, they were found to significantly quench the fluorescence of lipase, suggesting their strong affinities with this enzyme, which was further evidenced by molecular docking. Ligand fishing based on LMNPs shows great power for fast screening and identification of lipase inhibitors present in edible and medicinal plants.

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Section: Introductionmentioning

confidence: 99%

Lipase Ligands in Nelumbo nucifera Leaves and Study of Their Binding Mechanism

Zhu

Jia

Liu

et al. 2014

J. Agric. Food Chem.

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“…Nelumbo nucifera is a common edible and medicinal plant used in Asia for the treatment of diarrhea, bleeding, fever, and infection [ 11 - 13 ]. Extracts from Nelumbo nucifera have been shown to exert antioxidant or free radical scavenging activities [ 12 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of armepavine against hepatic fibrosis in rats

Weng¹,

Shen²,

Chiu³

et al. 2009

J Biomed Sci

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Activation of hepatic stellate cells (HSCs) plays a crucial role in liver fibrogenesis. armepavine (Arm, C 19 H 23 O 3 N), an active compound from Nelumbo nucifera, has been shown to exert immunosuppressive effects on T lymphocytes and on lupus nephritic mice. The aim of this study was to investigate whether Arm could exert anti-hepatic fibrogenic effects in vitro and in vivo. A cell line of rat HSCs (HSC-T6) was stimulated with tumor necrosis factor-α (TNF-α) or lipopolysaccharide (LPS) to evaluate the inhibitory effects of Arm. An in vivo therapeutic study was conducted in bile duct-ligated (BDL) rats. BDL rats were given Arm (3 or 10 mg/kg) by gavage twice daily for 3 weeks starting from the onset of BDL. Liver sections were taken for fibrosis scoring, immuno-fluorescence staining and quantitative real-time mRNA measurements. In vitro, Arm (1-10 μM) concentration-dependently attenuated TNF-α-and LPS-stimulated α-SMA protein expression and AP-1 activation by HSC-T6 cells without adverse cytotoxicity. Arm also suppressed TNF-α-induced collagen collagen deposition, NFκB activation and MAPK (p38, ERK1/2, and JNK) phosphorylations. In vivo, Arm treatment significantly reduced plasma AST and ALT levels, hepatic α-SMA expression and collagen contents, and fibrosis scores of BDL rats as compared with vehicle treatment. Moreover, Arm attenuated the mRNA expression levels of col 1α2, TGF-β1, TIMP-1, ICAM-1, iNOS, and IL-6 genes, but up-regulated metallothionein genes. Our study results showed that Arm exerted both in vitro and in vivo antifibrotic effects in rats, possibly through anti-NF-κB activation pathways.

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Nelumbo nucifera Gaertn.

Cited by 2 publications

References 22 publications

Lipase Ligands in Nelumbo nucifera Leaves and Study of Their Binding Mechanism

Lipase Ligands in Nelumbo nucifera Leaves and Study of Their Binding Mechanism

Inhibitory effects of armepavine against hepatic fibrosis in rats

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