Neisseria zalophi sp. nov., isolated from oral cavity of California sea lions (Zalophus californianus)

Volokhov, Dmitriy V.; Amselle, Megan; Bodeis-Jones, Sonya; Delmonte, Pierluigi; Zhang, Shuping; Davidson, Maureen K.; Gulland, Frances M. D.; Chizhikov, Vladimir E.

doi:10.1007/s00203-018-1499-x

“…recovered from humans and a number of domestic and experimental animals 1 . Reports from wild animals are less common, however, Neisseria zalophili was described recently from the oral cavity of wild California sea lions 2 , suggesting that pinnipeds may similarly harbour members of the Neisseria genus.…”

Section: Introductionmentioning

confidence: 99%

Forensic microbiology reveals that Neisseria animaloris infections in harbour porpoises follow traumatic injuries by grey seals

Foster¹,

Whatmore²,

Dagleish

³

et al. 2019

Sci Rep

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Neisseria animaloris is considered to be a commensal of the canine and feline oral cavities. It is able to cause systemic infections in animals as well as humans, usually after a biting trauma has occurred. We recovered N. animaloris from chronically inflamed bite wounds on pectoral fins and tailstocks, from lungs and other internal organs of eight harbour porpoises. Gross and histopathological evidence suggest that fatal disseminated N. animaloris infections had occurred due to traumatic injury from grey seals. We therefore conclude that these porpoises survived a grey seal predatory attack, with the bite lesions representing the subsequent portal of entry for bacteria to infect the animals causing abscesses in multiple tissues, and eventually death. We demonstrate that forensic microbiology provides a useful tool for linking a perpetrator to its victim. Moreover, N. animaloris should be added to the list of potential zoonotic bacteria following interactions with seals, as the finding of systemic transfer to the lungs and other tissues of the harbour porpoises may suggest a potential to do likewise in humans.

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“… Strains: 1, 10023 T ; 2, 10010; 3, Neisseria zalophi CSL 7565 T (Volokhov et al 2018 ); 4, Neisseria wadsworthii WC05-9715 T ; 5, Neisseria canis ATCC 14687 T . Values are percentages of total fatty acids.…”

Section: Resultsmentioning

confidence: 99%

“…Genes with greater nucleotide sequence diversity than the 16S rRNA gene, including rplF (encoding 50S ribosomal protein L6), argF (ornithine carbamoyltransferase), recA (DNA recombination/repair protein RecA), rpoB (DNA-directed RNA polymerase subunit beta), rpoD (RNA polymerase sigma factor RpoD), polI (DNAdirected DNA polymerase I), ribII (ribonuclease II), aspS (aspartate-tRNA ligase), gyrB (DNA gyrase subunit B) and porin precursor ( por ) exist in all Neisseria species (Volokhov et al 2018 ). As in previous studies, similarities for these genes between strain 10023 T and closely related Neisseria type strains were analysed.…”

Section: Methodsmentioning

confidence: 99%

“…There are currently 30 species and 3 subspecies in the genus Neisseria ( http://www.Bacterio.net/Neisseria.html ), among which Neisseria meningitidis and Neisseria gonorrhoeae are well-known human pathogens, causing bacterial meningitis and gonorrhoea, respectively (Virji 2009 ). Other species in the genus Neisseria may be associated with human and animal infections, having been isolated from animals, such as ducks (Murphy et al 2005 ), mice (Weyand et al 2016 ), sea lions (Volokhov et al 2018 ) and clinical specimens or nonviable eggs (Hansen et al 2017 ; Wolfgang et al 2011 , 2013 ; Wroblewski et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Neisseria chenwenguii sp. nov. isolated from the rectal contents of a plateau pika (Ochotona curzoniae)

Gui

¹

,

Yang

²

,

Lai

³

et al. 2019

Antonie van Leeuwenhoek

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Two Gram-stain negative, catalase positive, coccus shaped bacteria, designated 10023 T and 10010, were isolated from the rectal contents of a plateau pika ( Ochotona curzoniae ) in Qinghai–Tibet Plateau, China. Based on 16S rRNA gene sequence analysis, phylogenetic trees showed that these two isolates (10023 T , 10010) group with members of the genus Neisseria . Additionally, these two isolates exhibited high 16S rRNA gene sequence similarity with Neisseria zalophi CSL 7565 T (96.98%), Neisseria wadsworthii WC 05-9715 T (96.92%) and Neisseria canis ATCC 14687 T (96.79%). Further phylogenetic analysis based on the rplF gene showed that these two novel strains can be easily discriminated from phylogenetically closely related species. Optimal growth was found to occur on BHI agar with 5% defibrinated sheep blood at 37 °C and growth was also observed on nutrient agar, Columbia blood agar and chocolate agar plates; however, growth was not observed on MacConkey agar after 7 days. The major cellular fatty acids of these strains were identified as C 16:0 and C 16:1 ω7c/C 16:1 ω6c. The complete genome size of the type strain 10023 T is 2,496,444 bp, with DNA G+C content of 54.0 mol %. The average nucleotide identity values were 73.5–79.3% between isolate 10023 T and reference Neisseria spp. Based on polyphasic analysis, these isolates (10023 T and 10010) are considered to represent a novel species in the genus Neisseria , for which the name Neisseria chenwenguii sp. nov. is proposed. The type strain is 10023 T (= DSM 103440 T = CGMCC 1.15736 T ). Electronic supplementary material The online version of this article (10.1007/s10482-019-01234-2) contains supplementary material, which is available to authorized users.

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“…detection (GC-FID), as previously described [52]. Briefly, the strain was grown in Columbia broth supplemented with 10 % of fetal bovine serum at 37.0±1.0 °C for 3 days, and prepared for GC analysis [53].…”

Section: Phenotypic Characterization and Chemotaxonomymentioning

confidence: 99%

Oceanivirga miroungae sp. nov., isolated from oral cavity of northern elephant seal (Mirounga angustirostris)

Volokhov

¹

,

Blom

²

,

Amselle

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

Self Cite

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Two independent strains of a Leptotrichia species (ES3154-GLUT and ES2714_GLU) were isolated from the oral cavity of northern elephant seals (Mirounga angustirostris) that were admitted to The Marine Mammal Centre facilities in California, USA. The strains were isolated from oral swabs by cultivation in PPLO broth supplemented with serum, penicillin and colistin in anaerobic conditions. The strains were Gram-negative, pleomorphic, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile rods/coccobacilli in short chains. The 16S rRNA gene sequence of these strains shared 94.42 % nucleotide similarity with Oceanivirga salmonicida AVG 2115T but demonstrated ≤86.00–92.50 % nucleotide similarity to the 16S rRNA genes of other species of the family Leptotrichiaceae . The genome was sequenced for strain ES3154-GLUT. Average nucleotide identity values between strain ES3154-GLUT and 15 type strain genomes from the family Leptotrichiaceae ranged from 66.74 % vs. Sebaldella termitidis to 73.35 % vs. O. salmonicida . The whole genome phylogeny revealed that the novel species was most closely related to O. salmonicida AVG 2115T. This relationship was also confirmed by nucleotide similarity and multilocus phylogenetic analyses employing various housekeeping genes (partial 23S rRNA, rpoB, rpoC, rpoD, polC, adh, gyrA and gyrB genes). Chemotaxonomic and phenotypical features of strain ES3154-GLUT were in congruence with closely related members of the family Leptotrichiaceae , represented by similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis was capable to clearly discriminate strain ES3154-GLUT from all currently described taxa of the family Leptotrichiaceae . Based on these data, we propose a novel species of the genus Oceanivirga , for which the name Oceanivirga miroungae sp. nov. is proposed with the type strain ES3154-GLUT (=DSM 109740T=CCUG 73653T=ATCC TSD-189T=NCTC 14411T) and one representative strain ES2714_GLU. The G+C content is 26.82 %, genome size is 1 356 983 bp.

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Neisseria zalophi sp. nov., isolated from oral cavity of California sea lions (Zalophus californianus)

Cited by 25 publications

References 38 publications

Forensic microbiology reveals that Neisseria animaloris infections in harbour porpoises follow traumatic injuries by grey seals

Forensic microbiology reveals that Neisseria animaloris infections in harbour porpoises follow traumatic injuries by grey seals

Neisseria chenwenguii sp. nov. isolated from the rectal contents of a plateau pika (Ochotona curzoniae)

Oceanivirga miroungae sp. nov., isolated from oral cavity of northern elephant seal (Mirounga angustirostris)

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