Neiella holothuriorum sp. nov., isolated from the gut of a sea cucumber Apostichopus japonicus

et al. 2023

Arch Microbiol

A Gram-stain-negative, rod-shaped bacterium, designated HB171785 T , was isolated from soil sample collected from Qishui Bay, Hainan, China. The strain grew optimally at pH 7-8, 40-45°C and with NaCl 3-4%. The predominant isoprenoid quinone was found to be Q-8 and the major fatty acids were C 16:0 , C 16:1 ω7c / C 16:1 ω6c, C 18:1 ω7c / C 18:1 ω6c and C 12:0 3OH. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The size of the draft genome was 4.32 Mbp with G + C content 49.7%. Phylogenetic analysis of 16S rRNA gene sequence indicated that strain HB171785 T belonged to the order Alteromonadales, and the closest phylogenetically related species were Neiella marina j221 T , "Neiella holothuriorum" 126 and Echinimonas agarilytica LMG2520 T with the similarities of 98.2, 96.0 and 95.0%, respectively. The phylogenetic tree based on 16S rRNA genes and phylogenomic tree based on core genomes showed that strain HB171785 T clustered together with N. marina j221 T , with the highest values of average nucleotide identity (82.9%) and digital DNA-DNA hybridization (25.4%). The combined phylogenetic relatedness, phenotypic and genotypic features supported the conclusion that strain HB171785 T represents a novel species of the genus Neiella, for which the name Neiella litorisoli sp. nov. is proposed. The type strain is HB171785 T (= MCCC 1K04625 T = KCTC 82319 T ).

Section: Phylogenetic Analysismentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Neiella litorisoli sp. nov., an alginate lyase: producing bacterium from South China Sea, and proposal of Echinimonadaceae fam. nov. in the order Alteromonadales

et al. 2023

Arch Microbiol

“…The predominant polar lipids are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and Q-8 is the predominant respiratory quinone. Members of the order Alteromonadales have been isolated from diverse marine samples and NaCl is required for growth, such as from sea cucumber (Du et al 2013;Bai et al 2022), sea urchin (Nedashkovskaya et al 2013), shell sh (Ivanova et al 2002), coral (Li et al 2013), marine sand (Park et al 2011; Zhang et al 2014) and coast sediment (Nakagawa et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Neiella litorisoli sp. nov., an alginate lyase – excreting bacterium from South China Sea

et al. 2022

Preprint

A Gram-stain-negative, rod-shaped bacterium, designated HB171785 T , was isolated from soil sample collected from Qishui Bay, Hainan, China. The strain grew optimally at pH 7-8, 40-45°C and with NaCl 3-4%. The predominant isoprenoid quinone was found to be Q-8 and the major fatty acids were C 16:0 , C 16:1 ω7c / C 16:1 ω6c, C 18:1 ω7c / C 18:1 ω6c and C 12:0 3OH. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The size of the draft genome was 4.32 Mbp with G + C content 49.7%. Phylogenetic analysis of 16S rRNA gene sequence indicated that strain HB171785 T belonged to the order Alteromonadales, and the closest phylogenetically related species were Neiella marina j221 T , "Neiella holothuriorum" 126 T and Echinimonas agarilytica LMG2520 T with the similarities of 98.2, 96.0 and 95.0%, respectively. The highest values of average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH) and average amino acid identity (AAI) were found to be 82.9, 25.4 and 89.2%, respectively, between the isolate and its closest neighbor Neiella marina j221 T . The combined phylogenetic relatedness, phenotypic and genotypic features supported the conclusion that strain HB171785 T represents a novel species of the genus Neiella, for which the name Neiella litorisoli sp. nov. is proposed. The type strain is HB171785 T (= MCCC 1K04625 T = KCTC 82319 T ).

Neiella litorisoli sp. nov., an alginate lyase – producing bacterium from South China Sea

et al. 2022

Preprint

A Gram-stain-negative, rod-shaped bacterium, designated HB171785T, was isolated from soil sample collected from Qishui Bay, Hainan, China. The strain grew optimally at pH 7–8, 40–45°C and with NaCl 3–4%. The predominant isoprenoid quinone was found to be Q-8 and the major fatty acids were C16:0, C16:1 ω7c / C16:1 ω6c, C18:1 ω7c / C18:1 ω6c and C12:0 3OH. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The size of the draft genome was 4.32 Mbp with G + C content 49.7%. Phylogenetic analysis of 16S rRNA gene sequence indicated that strain HB171785T belonged to the order Alteromonadales, and the closest phylogenetically related species were Neiella marina j221T, “Neiella holothuriorum” 126 and Echinimonas agarilytica LMG2520T with the similarities of 98.2, 96.0 and 95.0%, respectively. The phylogenetic tree based on 16S rRNA genes and phylogenomic tree based on core genomes showed that strain HB171785T clustered together with N. marina j221T, with the highest values of average nucleotide identity (82.9%) and digital DNA-DNA hybridization (25.4%). The combined phylogenetic relatedness, phenotypic and genotypic features supported the conclusion that strain HB171785T represents a novel species of the genus Neiella, for which the name Neiella litorisoli sp. nov. is proposed. The type strain is HB171785T (= MCCC 1K04625T = KCTC 82319T).