Negative regulation of stress‐induced matrix metalloproteinase‐9 by Sirt1 in skin tissue

Lee, Ji‐Seon; Park, Keung-Young; Min, Hyang Ki; Lee, Seung-Jae; Kim, Jin Ju; Choi, Joon-Seok; Kim, Won‐Serk; Cha, Hyuk‐Jin

doi:10.1111/j.1600-0625.2010.01129.x

Cited by 47 publications

(36 citation statements)

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“…7,15) In line with this, modulation of MAPK activity, especially p38 MAPK by number of small molecules has been suggested to be a possible strategy to suppress the induction of MMP-9 after stress conditions. 15,16) During the screening of plant extracts using MMP-9 inhibition assay, the extract of Citrus unshiu (SWINGLE) MARCOW (Rutaceae) peel was found to inhibit MMP-9 expression.…”

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confidence: 91%

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Nobiletin Suppresses MMP-9 Expression through Modulation of p38 MAPK Activity in Human Dermal Fibrobalsts

Kim

Korm

Kim

et al. 2014

Biological & Pharmaceutical Bulletin

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We aimed to identify a novel flavonoid from the in-house natural products to suppress matrix metalloproteases (MMPs), which is responsible for degradation of collagen and other extracellular matrix proteins. Total eight natural products were screened for identification of a novel MMP-9 suppressor using MMP-9 reporter system, where the prompt initial screening with multiple samples is readily examined. Among the extracts used in the present study, one extract (Citrus unshiu) was found active in this assay system. Furthermore, three representative flavonoids in this active extract of Citrus unshiu peel were tested in MMP-9 reporter system. Nobiletin (NB) of the tested flavonoids suppressed MMP-9 expression without cytotoxicity, which was validated by both real-time polymerase chain reaction (PCR) and zymography analyses. Sustained p38 mitogen activated protein kinase (MAPK) activity, closely associated with induction of MMP-9 under stress condition, was markedly reduced by NB treatment, which implies that modulation of p38MAPK by nobiletin is responsible for reduction of MMP9 expression. Hence, nobiletin, identified from MMP-9 reporter system based screening, may be further applied for the purpose of delaying collagen degradation in skin fibroblasts.Key words Citrus unshiu; nobiletin; flavonoid; matrix metalloprotease 9; p38 mitogen activated protein kinase (MAPK)The integrity of skin connective tissue, which is important for the elasticity of the skin, relies on the levels of dermal extracellular matrix (ECM) proteins, such as collagen type I and III, elastin, and fibronectin. Aging or chronic exposure to ultra violet (UV) causes loss of ECM and the consequent decreased resilience of skin, which are strongly associated with various phenotypes of aged skin (e.g. wrinkle formation). 1)Matrix metalloproteinases (MMPs), members of the zincdependent endoprotease family, serve as important enzyme in degrading dermal ECM.2) Of note, excess expression of MMPs in keratinocytes and dermal fibroblasts, under a variety of stress conditions such as UV radiation (UVR), and heat-shock, 3) which both are closely associated to the aged skin phenotypes, 4,5) contributes to the degradation of ECM proteins, leading to wrinkle formation in photo-aged skin. 6)Therefore, MMPs, induced by a variety of external stresses and capable of degrading collagen and other ECM proteins, have been widely studied as a target for relieving the appearance of aged skin. 7)Among them, gelatinase A (MMP-2) and gelatinase B (MMP-9) were increased by UV radiation in human dermal fibroblasts (hDFs) 8,9) and are responsible for degrading collagens, which are components of the epidermal basement membranes.10) Thereby, the increased activity or expression of gelatinases induced by UV radiation to cause disruption of the basement membrane is considered to be a main cause of skin photoaging, 11,12) which can provide a rationale to target the gelatinases to prevent UV induced wrinkle formation. 7,12) In special, MMP-9 expression is dependent upon activator protei...

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confidence: 91%

“…7,12) In special, MMP-9 expression is dependent upon activator protein 1 (AP-1) transcriptional activity, 13) which is regulated by mitogen activated protein kinases (MAPKs).…”

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confidence: 99%

Nobiletin Suppresses MMP-9 Expression through Modulation of p38 MAPK Activity in Human Dermal Fibrobalsts

Kim

Korm

Kim

et al. 2014

Biological & Pharmaceutical Bulletin

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“…Resveratrol, a natural product-derived polyphenolic compound, has been widely used in the cosmetics and pharmaceutical industries because of its pharmacological activities, which include antitumor, anti-inflammation, and anti-aging effects . Recent reports have shown that resveratrol produced therapeutic effects against hyperplastic skin disorders by inhibiting keratinocyte proliferation , inhibited skin photoaging by acting as a Sirt-1 agonist (Lee et al, 2010), and showed anti-cancer effects in human melanoma cell lines (Niles et al, 2003). The anti-melanogenic effects of resveratrol and resveratrol analogs have already been reported by many other groups (Ohguchi et al, 2003;Song et al, 2012;Bae et al, 2013;Park and Boo, 2013;Park et al, 2014).…”

Section: Introductionmentioning

confidence: 76%

Resveratrol-Enriched Rice Down-Regulates Melanin Synthesis in UVB-Induced Guinea Pigs Epidermal Skin Tissue

Lee¹,

Seo²,

Ho³

et al. 2014

Biomol Ther

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. www.biomolther.orgSynthetic compounds that are used in the clinic to regulate skin hyperpigmentation, such as arbutin, hydroquinone, and kojic acid, are only moderately effective. But, their use is limited by side effects. As part of an effort to overcome the limitations, we developed resveratrol-enriched rice (RR) using genetic engineering technique. Each of resveratrol and rice has been reported to produce anti-melanogenic effects. Therefore, we hypothesized that RR would show more anti-melanogenic effects than those of resveratrol or rice alone. Anti-melanogenic effect of RR was done by using melan-a mouse melanocytes. The depigmenting efficacy was then observed following topical application of the RR to UVB-stimulated hyperpigmented dorsal skin of guinea pigs. Treatment with RR extract resulted a 21.4 ± 0.7% decrease in tyrosinase expression at melan-a cells. Colorimetric analysis showed a significantly lower depigmenting value by day 9 following treatment with RR in UVB-irradiated guinea pigs the dorsal skin (p<0.01), indicating that RR produced a depigmentation effect. By staining with Fontana-Masson stain, we found that the RR-treated group had more effect histopathologically in epidermal melanin production than resveratrol or rice alone-treated group. RR was associated with reduction in the levels of microphthalmia-associated transcription factor (MITF), and downregulation of tyrosinase and tyrosinaserelated protein (TRP-2) expression, leading to inhibit epidermal melanin production by western blot analysis. This study suggests that the resveratrol-enriched rice may be a promising candidate in regulating skin pigmentation with UVB exposure.

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“…Their increased expression will cause an extracellular matrix metabolism disorder, leading to ventricular remodeling. Evidence shows that MMP-1 and MMP-3 expression is increased after siRNA silencing of the SIRT1 gene, whereas SIRT1 agonists suppress the elevation in MMP-1 [91,92]. Resveratrol can activate SIRT1 and thereby inhibit MMP-9 expression [91,92].…”

Section: Anti-myocardial Remodeling Effectmentioning

confidence: 95%

SIRT1: Role in cardiovascular biology

2015

Clinica Chimica Acta

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Negative regulation of stress‐induced matrix metalloproteinase‐9 by Sirt1 in skin tissue

Cited by 47 publications

References 37 publications

Nobiletin Suppresses MMP-9 Expression through Modulation of p38 MAPK Activity in Human Dermal Fibrobalsts

Nobiletin Suppresses MMP-9 Expression through Modulation of p38 MAPK Activity in Human Dermal Fibrobalsts

Resveratrol-Enriched Rice Down-Regulates Melanin Synthesis in UVB-Induced Guinea Pigs Epidermal Skin Tissue

SIRT1: Role in cardiovascular biology

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