Negative Regulation of Adipogenesis by Kaempferol, a Component of &lt;i&gt;Rhizoma Polygonati falcatum&lt;/i&gt; in 3T3-L1 Cells

Park, Ui-Hyun; Jeong, Ji‐Cheon; Jang, Jae‐Sik; Sung, Mi-Ran; Youn, HyeSook; Lee, Sook-Jeong; Kim, Eun-Joo; Um, Soo‐Jong

doi:10.1248/bpb.b12-00254

Cited by 50 publications

(36 citation statements)

References 40 publications

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“…Several plant extracts and compounds that show an inhibitory effect on body weight gain via suppression of PPARγ expression have been reported (24–27). As previously suggested, kaempferol, a type of flavonol shown to be a PPARγ antagonist, exerts its anti-obesity effects partly through the down-regulation of PPARγ (28). BBR potently inhibits the differentiation of 3T3-L1 preadipocytes by inhibiting the mRNA and protein levels of PPARγ and C/EBPα and their upstream regulators (29).…”

Section: Discussionmentioning

confidence: 93%

Rosehip Extract Inhibits Lipid Accumulation in White Adipose Tissue by Suppressing the Expression of Peroxisome Proliferator-activated Receptor Gamma

Nagatomo¹,

Nishida²,

Matsuura³

et al. 2013

JFN

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Recent studies have shown that Rosa canina L. and tiliroside, the principal constituent of its seeds, exhibit anti-obesity and anti-diabetic activities via enhancement of fatty acid oxidation in the liver and skeletal muscle. However, the effects of rosehip, the fruit of this plant, extract (RHE), or tiliroside on lipid accumulation in adipocytes have not been analyzed. We investigated the effects of RHE and tiliroside on lipid accumulation and protein expression of key transcription factors in both in vitro and in vivo models. RHE and tiliroside inhibited lipid accumulation in a dose-dependent manner in 3T3-L1 cells. We also analyzed the inhibitory effect of RHE on white adipose tissue (WAT) in high-fat diet (HFD)-induced obesity mice model. Male C57BL/6J mice were fed HFD or HFD supplemented with 1% RHE (HFDRH) for 8 weeks. The HFDRH-fed group gained less body weight and had less visceral fat than the HFD-fed group. Liver weight was significantly lower in the HFDRH-fed group and total hepatic lipid and triglyceride (TG) content was also reduced. A significant reduction in the expression of peroxisome proliferator-activated receptor gamma (PPARγ) was observed in epididymal fat in the HFDRH-fed group, in comparison with controls, through Western blotting. These results suggest that downregulation of PPARγ expression is involved, at least in part, in the suppressive effect of RHE on lipid accumulation in WAT.

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Section: Discussionmentioning

confidence: 93%

Rosehip Extract Inhibits Lipid Accumulation in White Adipose Tissue by Suppressing the Expression of Peroxisome Proliferator-activated Receptor Gamma

Nagatomo¹,

Nishida²,

Matsuura³

et al. 2013

JFN

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“…26) H460, H1299, and A549 cells were seeded in 96-well plates at a density of 3000/well for 24 h, and treated with different concentrations of quercetin (0-200 µm) for 72 h. Cells were then incubated with MTT reagent (2 mg/ mL, 50 µL) for 4 h. The effect of quercetin on cell proliferation was measured by incubating cells with 50 µm quercetin at different time points. The fraction of living cells was measured using an enzyme-linked immunosorbent assay plate reader as the optical density at 540 nm.…”

Section: Methodsmentioning

confidence: 99%

Quercetin Potentiates Apoptosis by Inhibiting Nuclear Factor-kappaB Signaling in H460 Lung Cancer Cells

Youn

Jeong

et al. 2013

Biological & Pharmaceutical Bulletin

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The herbal flavonoid quercetin inhibits the growth of various cancer cells, but how it affects human cancer cells, particularly lung cancer cells, is unclear. We investigated the anticancer activity of quercetin and the underlying molecular mechanisms in non-small cell lung cancer (NSCLC) cells. Quercetin strongly inhibited cell proliferation, and increased sub-G1 and apoptotic cell populations regardless of p53 status. Quercetin-induced apoptosis was verified by caspase cleavage, Hoechst staining, trypan blue exclusion, and DNA fragmentation assays. Microarray analysis using H460 cells indicated that quercetin increased the expression of genes associated with death receptor signaling tumor necrosis factor-related apoptosis-inducing ligand receptor (TRAILR), caspase-10, interleukin (IL) 1R DNA fragmentation faotor 45 (DFF45), tumor necrosis factor receptor (TNFR) 1, FAS, inhibitor of kappaBalpha (IκBα)) and cell cycle inhibition growth arrest and DNA-damage inducible 45 (GADD45), p21 Cip1), but decreased the expression of genes involved in nuclear factor (NF)-kappaB activation (NF-κB, IKKα). Further validation assays confirmed that quercetin inhibited growth by suppressing NF-κB and by increasing the expression of death receptors and cell cycle inhibitors. Taken together, these findings suggest that quercetin may be useful in the prevention and therapy of NSCLC.Key words quercetin; lung cancer; apoptosis; death receptor; nuclear factor-kappaB pathway Lung cancer is the leading cause of cancer death in many developed countries because of its poor prognosis. Non-small cell lung cancer (NSCLC) accounts for 85% of lung cancer cases, of which 70% show advanced-stage disease at the time of diagnosis.1,2) Although various chemical drugs have been developed, 3,4) frequent chemoresistance and side effects in patients with NSCLC require the development of new preventive and therapeutic agents. High intake of flavonoids from fruits and vegetables is associated with a low risk of various cancers, 5-10) including lung cancer. 11,12) Of these polyphenolic flavonoids, quercetin is readily found in fruits, vegetables, green and black tea, and various medical plants such as Euonymus alatus (Thunb.) Sieb. Quercetin can act as either an antioxidant or a prooxidant depending on its concentration (1-40 µm or 40-100 µm, respectively). 13,14) Numerous studies have reported a broad range of pharmacological properties of quercetin, including benefits for inflammation, 15) atherosclerosis, 16) hypertension, 17) and neurodegeneration. 18,19) In addition to these beneficial effects, quercetin plays preventive and therapeutic roles in various types of cancer and cancer cells. [20][21][22][23][24][25] However, the anticancer effect of quercetin in NSCLC cells has been rarely addressed, and the underlying molecular mechanism remains to be determined.Here, we investigated the growth inhibitory role of quercetin in three NSCLC cell lines and found that its effect is p53-independent and triggered by apoptosis. Subsequent microarray analysis usin...

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“…Another plant species, Aspalathus linearis , contains quercetin as a major component and inhibits adipogenesis and the accumulation of triglycerides in adipocytes [31]. Studies carried out with kaempferol revealed that this flavonoid reduces adipogenesis in 3T3-L1 cells [32]. In addition, Bhattacharya et al [33] demonstrated that flower extracts containing quercetin and kaempferol reduced the accumulation of fat in Caenorhabditis elegans, a model for obesity.…”

Section: Discussionmentioning

confidence: 99%

Antiobesity Effects of Hydroethanolic Extract of Jacaranda decurrens Leaves

Antunes

Baldivia

Rocha

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Obesity is a worldwide epidemic that reduces life expectancy; therefore, the search for new alternative and effective treatments is ongoing. The aim of the present investigation was to identify the chemical compounds in the hydroethanolic extract of leaves of Jacaranda decurrens subsp. symmetrifoliolata and to evaluate their toxicity and antiobesity effects. High-performance liquid chromatography was used to identify the chemical constituents, and acute toxicity was evaluated in rats treated with doses of 2 and 5 g·kg−1 body mass. The antiobesity effect was determined in rats with hypercaloric diet-induced obesity. Our results revealed the presence of compounds, such as jacaric, ursolic, and oleic acids, as well as luteolin, quercetin, and kaempferol, in the extract. The acute toxicity tests revealed that rats treated with elevated doses of the extract showed no signs of toxicity. The extract induced reduction in total body mass and the white adipose tissue depots. The obese rats treated with the extract showed an increased fluid intake and feces excretion while their serum total cholesterol and triglyceride levels decreased compared to those in the controls, without any hematological changes. Taken together, the results showed that the constituents of J. decurrens extracts included phenolic compounds and exhibited antiobesity effects with no toxicity.

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Negative Regulation of Adipogenesis by Kaempferol, a Component of <i>Rhizoma Polygonati falcatum</i> in 3T3-L1 Cells

Cited by 50 publications

References 40 publications

Rosehip Extract Inhibits Lipid Accumulation in White Adipose Tissue by Suppressing the Expression of Peroxisome Proliferator-activated Receptor Gamma

Rosehip Extract Inhibits Lipid Accumulation in White Adipose Tissue by Suppressing the Expression of Peroxisome Proliferator-activated Receptor Gamma

Quercetin Potentiates Apoptosis by Inhibiting Nuclear Factor-kappaB Signaling in H460 Lung Cancer Cells

Antiobesity Effects of Hydroethanolic Extract of Jacaranda decurrens Leaves

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