Negative-Pressure Wound Therapy Promotes Wound Healing by Enhancing Angiogenesis Through Suppression of NLRX1 via miR-195 Upregulation

Abstract

Negative-pressure wound therapy (NPWT) is one of the most advanced therapeutic methods in the treatment of various hard-to-heal acute and refractory chronic wounds. Recent emerging evidence points to a role of the microRNA-mediated regulation of angiogenesis in ischemic tissues, and a series of microRNAs associated with angiogenesis have been successively identified. In this study, we found that miR-195 expression was significantly upregulated and the microvessel density (MVD) was increased in granulation tissue collected 7 days after NPWT compared with those in the pre-NPWT tissue. Moreover, the expression of NLRX1, the potential target gene of miR-195, was down-regulated in post-NPWT compared with that in pre-NPWT tissue. Significant negative correlations were detected between miR-195 and NLRX1 expression levels ( r = -.856, P < .001) and between NLRX1 expression and MVD ( r = -.618, P < .05), whereas miR-195 expression was positively correlated with MVD in the granulation tissue ( r = .630, P < .05). In summary, NPWT may suppress NLRX1 expression through the upregulation of miR-195 expression, thus efficaciously promoting angiogenesis in the granulation tissue to enhance wound healing.

Israeli Innovations in the Field of Plastic Surgery

“…42,43 Similarly, it promotes wound healing by stimulating angiogenesis, forming granulation tissue, and removing healing inhibitors. 42,44 The Vcare α system is also a novel vacuum therapy device developed by IVT Medical Ltd. This negative pressure device can be used with TopClosure and was designed primarily to decontaminate the wound and remove excess fluid (►Fig.…”

Behind the Scenes: Nod-Like Receptor X1 Controls Inflammation and Metabolism

“…NLRX1 protected against inflammation in mice in a smoke-induced chronic obstructive pulmonary disease model by inhibiting MAVS-RIG-I pathway, or by inhibiting MAVS-dependent NLPR3 inflammasome activation in hypoxia-induced cardiomyocytes in two different models of myocardial injury (Li et al, 2016;Tong et al, 2020). Although most often linked to increased inflammation and lesion severity, the loss of NLRX1 also promoted healing by enhancing angiogenesis through miR-195 upregulation (Liu et al, 2018), or by increased expression of wound healing factors such as epidermal growth factor (EGF) and transforming growth factor beta 1 (TGFb1) following injury (Tattoli et al, 2016).…”

Photoacoustic monitoring of angiogenesis predicts response to therapy in healing wounds

“…Studies have shown that elevated glucose levels in diabetic patients hinders angiogenesis resulting in diabetic ulcer formation, poor wound healing and limb loss 23,24 . Treatment protocols such as hyperbaric oxygen therapy, 25 negative pressure wound therapy 26 and debridement 27 can promote angiogenesis and improve healing outcomes. Hypertension can impair angiogenesis 28 .…”