Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects

Seo, Sang Gyo; Yeo, Ji Hyun; Kim, Ji Hye; Kim, Ji-Beom; Cho, Tae-Joon; Lee, Dong Yeon

doi:10.1038/emm.2013.129

Cited by 41 publications

(35 citation statements)

References 25 publications

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“…Based on this fact, the search for measures to inhibit toxin production and biofilm formation is an active area of clinical research. Recently, as an effective management of contaminated wounds, negative pressure wound therapy (NPWT) has been widely used in clinical laboratories [14, 15]. However, whether NPWT could reduce the bacterial load of wounds is still controversial.…”

Section: Introductionmentioning

confidence: 99%

Effect of Negative Pressure on Proliferation, Virulence Factor Secretion, Biofilm Formation, and Virulence-Regulated Gene Expression ofPseudomonas aeruginosaIn Vitro

Wang

et al. 2016

BioMed Research International

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Objective. To investigate the effect of negative pressure conditions induced by NPWT on P. aeruginosa. Methods. P. aeruginosa was cultured in a Luria–Bertani medium at negative pressure of −125 mmHg for 24 h in the experimental group and at atmospheric pressure in the control group. The diameters of the colonies of P. aeruginosa were measured after 24 h. ELISA kit, orcinol method, and elastin-Congo red assay were used to quantify the virulence factors. Biofilm formation was observed by staining with Alexa Fluor® 647 conjugate of concanavalin A (Con A). Virulence-regulated genes were determined by quantitative RT-PCR. Results. As compared with the control group, growth of P. aeruginosa was inhibited by negative pressure. The colony size under negative pressure was significantly smaller in the experimental group than that in the controls (p < 0.01). Besides, reductions in the total amount of virulence factors were observed in the negative pressure group, including exotoxin A, rhamnolipid, and elastase. RT-PCR results revealed a significant inhibition in the expression level of virulence-regulated genes. Conclusion. Negative pressure could significantly inhibit the growth of P. aeruginosa. It led to a decrease in the virulence factor secretion, biofilm formation, and a reduction in the expression level of virulence-regulated genes.

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Section: Introductionmentioning

confidence: 99%

Effect of Negative Pressure on Proliferation, Virulence Factor Secretion, Biofilm Formation, and Virulence-Regulated Gene Expression ofPseudomonas aeruginosaIn Vitro

Wang

et al. 2016

BioMed Research International

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“…A number of studies have demonstrated that wound healing can be improved by increasing the number of circulating SPCs and/or enhancing wound site recruitment (2,7,16,17). There also is evidence that some adjuncts to standard treatment such as negative pressure dressings and hyperbaric oxygen will mobilize SPCs to blood and may also modify regulatory protein content that improves vasculogenic function (11,15,18,19). Pharmacological agents that might be used incidental to DFU treatment or as an adjunct may also influence SPCs (20–32).…”

Section: Introductionmentioning

confidence: 99%

Measurements of CD34+/CD45-dim Stem Cells Predict Healing of Diabetic Neuropathic Wounds

et al. 2015

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Management of neuropathic foot ulcers in patients with diabetes (DFUs) has changed little over the past decade, and there is currently no objective method to gauge probability of successful healing. We hypothesized that studies of stem/progenitor cells (SPCs) in the early weeks of standard wound management could predict who will heal within 16 weeks. Blood and debrided wound margins were collected for 8 weeks from 100 patients undergoing weekly evaluations and treatment. SPC number and intracellular content of hypoxia-inducible factors (HIFs) were evaluated by flow cytometry and immunohistochemistry. More SPCs entered the bloodstream in the first 2 weeks of care in patients who healed (n = 37) than in those who did not (n = 63). Logistic regression demonstrated that the number of blood-borne SPCs and the cellular content of HIFs at study entry and the first-week follow-up visit predicted healing. Strong correlations were found among week-to-week assessments of blood-borne SPC HIF factors. We conclude that assays of SPCs during the first weeks of care in patients with DFUs can provide insight into how well wounds will respond and may aid with decisions on the use of adjunctive measures.

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“…A prior study reveals that the systemic endothelial progenitor cells (EPCs), considered as markers of healing and repair, are mobilized during NPWT in patients with diabetic foot wounds . Therefore, we hypothesize that the number of fibrocytes in diabetic wounds is also up‐regulated during NPWT and that mobilized fibrocytes might play an important role in the healing of intractable wounds.…”

Section: Introductionmentioning

confidence: 94%

Circulating fibrocyte mobilization in negative pressure wound therapy

Chen

Zhao

et al. 2017

J Cellular Molecular Medi

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Non-healing diabetic wounds are difficult to treat. They also create heavy financial burdens for both patients and society. Negative pressure wound therapy (NPWT) has been adopted to treat intractable wounds and has proved to be effective. However, the mechanisms that underlie the effects of this treatment are not entirely understood. Circulating fibrocytes are unique haematopoietic-derived stem cells that have been reported to play a pivotal role in wound healing. Here, we have investigated the effect of NPWT on fibrocyte mobilization and the role of fibrocyte mobilization in the healing of diabetic wounds during NPWT. We show that the NPWT group exhibited 2.6-fold to 12.1-fold greater numbers of tail vein-injected PKH-26-labelled fibrocytes in the diabetic wound sites compared with the control group. We also demonstrate that the fullthickness skin wounds treated with NPWT exhibit significantly reduced mRNA and protein expression, blood vessel density and proliferating cells when exogenous fibrocyte mobilization is inhibited. We speculate that systemic mobilization of fibrocytes during NPWT may be a mechanism for healing intractable wounds in a diabetic rat model experiment and that enhancement of cell mobilization may represent a potential treatment idea for intractable wound healing across all fields of surgery.

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Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects

Cited by 41 publications

References 25 publications

Effect of Negative Pressure on Proliferation, Virulence Factor Secretion, Biofilm Formation, and Virulence-Regulated Gene Expression ofPseudomonas aeruginosaIn Vitro

Effect of Negative Pressure on Proliferation, Virulence Factor Secretion, Biofilm Formation, and Virulence-Regulated Gene Expression ofPseudomonas aeruginosaIn Vitro

Measurements of CD34+/CD45-dim Stem Cells Predict Healing of Diabetic Neuropathic Wounds

Circulating fibrocyte mobilization in negative pressure wound therapy

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