Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis

Zhang, Sheng; Xie, Yuanlong; Yan, F.; Zhang, Yufeng; Yang, Zhiqiang; Chen, Zhe; Zhao, Yong; Huang, Zan; Cai, Lin; Deng, Zhouming

doi:10.1080/15548627.2021.2016231

Cited by 45 publications

(25 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the autophagy pathway, ULK1 has been proposed to be phosphorylated by AMPK at S556 (autophagy activation) or by mTOR at S757 (autophagy inhibition) to regulate autophagy in mammalian cells [ 54 – 56 ]. Zhang et al [ 57 ] revealed that the AMPK-ULK1-autophagy axis can be activated by negative pressure to promote osteoblast differentiation of rat mesenchymal stem cells and bone regeneration. Consistently, SFN treatment activated autophagy through the AMPK-ULK1 pathway, but not the mTOR signaling.…”

Section: Discussionmentioning

confidence: 99%

Sulforaphane prevents LPS-induced inflammation by regulating the Nrf2-mediated autophagy pathway in goat mammary epithelial cells and a mouse model of mastitis

Shao

Shen

Miao

et al. 2023

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Mastitis not only deteriorates the composition or quality of milk, but also damages the health and productivity of dairy goats. Sulforaphane (SFN) is a phytochemical isothiocyanate compound with various pharmacological effects such as anti-oxidant and anti-inflammatory. However, the effect of SFN on mastitis has yet to be elucidated. This study aimed to explore the anti-oxidant and anti-inflammatory effects and potential molecular mechanisms of SFN in lipopolysaccharide (LPS)-induced primary goat mammary epithelial cells (GMECs) and a mouse model of mastitis. Results In vitro, SFN downregulated the mRNA expression of inflammatory factors (tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6), inhibited the protein expression of inflammatory mediators (cyclooxygenase-2 (COX2), and inducible nitric oxide synthase (iNOS)) while suppressing nuclear factor kappa-B (NF-κB) activation in LPS-induced GMECs. Additionally, SFN exhibited an antioxidant effect by increasing Nrf2 expression and nuclear translocation, up-regulating antioxidant enzymes expression, and decreasing LPS-induced reactive oxygen species (ROS) production in GMECs. Furthermore, SFN pretreatment promoted the autophagy pathway, which was dependent on the increased Nrf2 level, and contributed significantly to the improved LPS-induced oxidative stress and inflammatory response. In vivo, SFN effectively alleviated histopathological lesions, suppressed the expression of inflammatory factors, enhanced immunohistochemistry staining of Nrf2, and amplified of LC3 puncta LPS-induced mastitis in mice. Mechanically, the in vitro and in vivo study showed that the anti-inflammatory and anti-oxidative stress effects of SFN were mediated by the Nrf2-mediated autophagy pathway in GMECs and a mouse model of mastitis. Conclusions These results indicate that the natural compound SFN has a preventive effect on LPS-induced inflammation through by regulating the Nrf2-mediated autophagy pathway in primary goat mammary epithelial cells and a mouse model of mastitis, which may improve prevention strategies for mastitis in dairy goats.

show abstract

Section: Discussionmentioning

confidence: 99%

Sulforaphane prevents LPS-induced inflammation by regulating the Nrf2-mediated autophagy pathway in goat mammary epithelial cells and a mouse model of mastitis

Shao

Shen

Miao

et al. 2023

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…NPWT has also been reported to enhance bone regeneration. Zhang et al reported that “NPWT promoted bone regeneration in vivo” and that “negative pressure treatment induced osteoblast differentiation in vitro.” 6 The patient received postamputation NPWT for 13 days. Therefore, NPWT may be effective in bone regeneration.…”

Section: Discussionmentioning

confidence: 99%

A Case of Fibula Regeneration after Below-the-knee Amputation in an Adult

Fukuba

Dogo

Saito

et al. 2023

Plastic and Reconstructive Surgery - Global Open

View full text Add to dashboard Cite

We report the case of an adult with fibula regeneration after below-the-knee amputation. Fibula regeneration conventionally occurs at the donor site of children after autogenous fibula transplantation when the periosteum is preserved. However, the patient was an adult, and the regenerated fibula was 7-cm long and grew directly from the stump. A 47-year-old man was referred to the plastic surgery department owing to stump pain. He had an open comminuted fracture of the right fibula and tibia due to a traffic accident when he was 44 years old and underwent below-the-knee amputation and negative pressure wound therapy for skin defects. The patient recovered and was able to walk using a prosthetic limb. Upon radiography, the fibula was found to have regenerated 7 cm directly from the stump. Pathological examination revealed that the regenerated fibula contained normal bone tissue and neurovascular bundles in the cortex. The periosteum, mechanical stimuli with limb proteases, and negative pressure wound therapy were suspected to have accelerated bone regeneration. He had no inhibitory factors for bone regeneration, including diabetes mellitus, peripheral arterial disease, or active smoking status. After the resection of the regenerated fibula, the patient was ambulatory without further bone regeneration or pain. This case report suggests that bone regeneration may occur even in adults. The surgeon should not leave any part of the periosteum behind in patients undergoing amputation. In adult amputees complaining of stump pain, the possibility of bone regeneration may be considered.

show abstract

“…Zhang et al reported that negative pressure could promote bone regeneration by promoting osteogenic differentiation. 17 Some studies have used decompression combined with negative pressure suction and pressure balls or other devices to deal with negative intracapsular pressure for a certain time every day, with good results. 18 , 19 …”

Section: Discussionmentioning

confidence: 99%