Negative Ion Ultraviolet Matrix-Assisted Laser Desorption Ionization Mass Spectrometry and Post Source Decay of Glycosyl Esters of Nucleoside Pyrophosphates

Abstract

Six different glycosyl esters of nucleoside pyrophosphates (monosaccharide nucleotides) were analyzed by means of matrix-assisted laser desorption/ionisation reflectron time-of-flight mass spectrometry (MALDI/RToF MS) in the negative ion mode. Several matrices were evaluated and 3-hydroxypicolinic acid as well as α-cyano-4-hydroxycinnamic acid (CHCA) turned out to be the matrices of choice applying the thin layer technique to obtain maximum sensitivity for deprotonated molecular ion detection and maximal fragmentation particular with CHCA. The determination of the molecular mass with a mass accuracy below 0.1% was feasible with sample amounts in the lower femtomole range applying a MALDI desk-top mass spectrometer. A further important refinement of this technique was the use of post source decay (PSD) fragment ion analysis with a curved field reflector (which means no stepping of the reflector voltage). Detailed structural information of the six selected monosaccharide nucleotides could be obtained with PSD and differences in the fragmentation pattern were used to distinguish them. This method (based on molecular mass and PSD fragment ion analysis) has been applied to verify the presence of a glycosyl ester of nucleoside pyrophosphate in samples from Saccharomyces cerevisiae. KeywordsMALDI; post source decay; mass spectrometry; carbohydrate biosynthesis; glycosyl ester of nucleoside pyrophosphate; negative ions; ToF

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

“…Negative ion mode has also been used to study the PSD fragmentation of a series of six glycosyl esters of nucleoside pyrophosphates and to verify the presence of one of these compounds in Saccharomyces cerevisiae (Heinrich et al, 2008).…”