Negative interaction of Staphylococcus aureus on Fusarium falciforme growth ocular isolates in an in vitro mixed biofilm

Bautista-Hernández, Luis Antonio; Gómez-Olivares, José Luís; Buentello-Volante, Beatríz; Domínguez-López, Alfredo; Garfias, Yonathan; Acosta-García, M. C.; Calvillo‐Medina, Rosa Paulina; Lucio, Víctor Manuel Bautista-de

doi:10.1016/j.micpath.2019.103644

Cited by 6 publications

(7 citation statements)

References 39 publications

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“…The particles were able to disrupt in a similar manner single-species or dual-species biofilms, but it was observed that adhesion of bacteria to Candida hyphae made their surface less accessible to antimicrobial molecules. This obstacle was already described for free antimicrobial molecules in dual-species biofilms of S. aureus and Fusarium falciforme [49]. This interaction between the microbial species was also observed for C. acnes et C. albicans [22] and could participate in the loss of activity of ANCs on C. albicans in the dual-species biofilm.…”

Section: Discussionsupporting

confidence: 57%

Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

et al. 2020

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Biofilm-related infections are a matter of concern especially because of the poor susceptibility of microorganisms to conventional antimicrobial agents. Innovative approaches are needed. The antibiofilm activity of extracts of cyanobacteria Arthrospira platensis, rich in free fatty acids, as well as of extract-loaded copper alginate-based nanocarriers, were studied on single- and dual-species biofilms of Candida albicans and Cutibacterium acnes. Their ability to inhibit the biofilm formation and to eradicate 24 h old biofilms was investigated. Concentrations of each species were evaluated using flow cytometry. Extracts prevented the growth of C. acnes single-species biofilms (inhibition > 75% at 0.2 mg/mL) but failed to inhibit preformed biofilms. Nanovectorised extracts reduced the growth of single-species C. albicans biofilms (inhibition > 43% at 0.2 mg/mL) while free extracts were weakly or not active. Nanovectorised extracts also inhibited preformed C. albicans biofilms by 55% to 77%, whereas the corresponding free extracts were not active. In conclusion, even if the studied nanocarrier systems displayed promising activity, especially against C. albicans, their efficacy against dual-species biofilms was limited. This study highlighted that working in such polymicrobial conditions can give a more objective view of the relevance of antibiofilm strategies by taking into account interspecies interactions that can offer additional protection to microbes.

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Section: Discussionsupporting

confidence: 57%

Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

et al. 2020

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“…This mycophagy event, generated by the bacteria in the HLFC model, has a direct impact over the fungal population, since bacteria are taking advantage of the fungal components for self-nutrition (Leveau and Preston, 2008). Mycophagy events have previously been reported for this Staphylococcus species in other in vitro fungal-bacterial models (Ikeda et al, 2007;Camarillo-Maŕquez et al, 2018;Bautista-Hernańdez et al, 2019). In summary, the correlation of the MTT values with the microscopic evidence obtained by SEM suggests that in this FBI on HLFCs, the prevalent microorganism is S. aureus.…”

Section: Discussionmentioning

confidence: 60%

“…Similarly, abundant amounts of extracellular material of single and mixed biofilms were demonstrated in the presence of the HLFC monolayers by EFM (Figures 5, 6). This qualitative technique of biofilm has been used by our research group for the detection of biomolecules that constitute the ECM (Ramıŕez Granillo et al, 2015;Gonzaĺez-Ramıŕez et al, 2016;Camarillo-Maŕquez et al, 2018;Bautista-Hernańdez et al, 2019). Other authors have reported that by using molecules to specifically eliminate ECM components, such as sodium periodate (that destroys carbohydrates), DNase (that digests DNA), and proteinase K (that digests proteins), it was possible to demonstrate that fluorochromes detect specifically such biomolecules (Baillie and Douglas, 2000;Chandra et al, 2001;Coŕdova-Alcańtara et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Microbial Warfare on Three Fronts: Mixed Biofilm of Aspergillus fumigatus and Staphylococcus aureus on Primary Cultures of Human Limbo-Corneal Fibroblasts

Ramírez-Granillo

Bautista-Hernández²,

Lucio³

et al. 2021

Front. Cell. Infect. Microbiol.

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BackgroundCoinfections with fungi and bacteria in ocular pathologies are increasing at an alarming rate. Two of the main etiologic agents of infections on the corneal surface, such as Aspergillus fumigatus and Staphylococcus aureus, can form a biofilm. However, mixed fungal–bacterial biofilms are rarely reported in ocular infections. The implementation of cell cultures as a study model related to biofilm microbial keratitis will allow understanding the pathogenesis in the cornea. The cornea maintains a pathogen-free ocular surface in which human limbo-corneal fibroblast cells are part of its cell regeneration process. There are no reports of biofilm formation assays on limbo-corneal fibroblasts, as well as their behavior with a polymicrobial infection.ObjectiveTo determine the capacity of biofilm formation during this fungal–bacterial interaction on primary limbo-corneal fibroblast monolayers.ResultsThe biofilm on the limbo-corneal fibroblast culture was analyzed by assessing biomass production and determining metabolic activity. Furthermore, the mixed biofilm effect on this cell culture was observed with several microscopy techniques. The single and mixed biofilm was higher on the limbo-corneal fibroblast monolayer than on abiotic surfaces. The A. fumigatus biofilm on the human limbo-corneal fibroblast culture showed a considerable decrease compared to the S. aureus biofilm on the limbo-corneal fibroblast monolayer. Moreover, the mixed biofilm had a lower density than that of the single biofilm. Antibiosis between A. fumigatus and S. aureus persisted during the challenge to limbo-corneal fibroblasts, but it seems that the fungus was more effectively inhibited.ConclusionThis is the first report of mixed fungal–bacterial biofilm production and morphological characterization on the limbo-corneal fibroblast monolayer. Three antibiosis behaviors were observed between fungi, bacteria, and limbo-corneal fibroblasts. The mycophagy effect over A. fumigatus by S. aureus was exacerbated on the limbo-corneal fibroblast monolayer. During fungal–bacterial interactions, it appears that limbo-corneal fibroblasts showed some phagocytic activity, demonstrating tripartite relationships during coinfection.

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“…Additionally, the bacterium Staphylococcus aureus strongly inhibited fungal conidiation, filamentation and consequently biofilm formation of A. fumigatus by direct cellecell contact [91]. This same bacterium was also able to negatively influence the filamentation and biofilm formation of the fungus Fusarium falciforme [92]. Most of these studies were performed within 24 h of interaction, however, with medium renewal and removal of planktonic cells, after that time, an increase in fungal viability could be observed, which could lead to its germination and development into a biofilm [87].…”

Section: Fungal-bacterial Interactions In Biofilmsmentioning

confidence: 99%

Occurrence of filamentous fungi in drinking water: their role on fungal-bacterial biofilm formation

Afonso

Simões

Lima

2021

Research in Microbiology

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Water is indispensable to life and safe and accessible supply must be available to all. The presence of microorganisms is a threat to this commitment. Biofilms are the main reservoir of microorganisms inside water distribution systems and they are extremely ecologically diverse. Filamentous fungi and bacteria can coexist inside these systems forming inter-kingdom biofilms. This review has the goal of summarizing the most relevant and recent reports on the occurrence of filamentous fungi in water distribution systems along with the current knowledge and gaps about filamentous fungal biofilm formation. Special focus is given on fungal-bacterial interactions in water biofilms.

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Negative interaction of Staphylococcus aureus on Fusarium falciforme growth ocular isolates in an in vitro mixed biofilm

Cited by 6 publications

References 39 publications

Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

Nanovectorized Microalgal Extracts to Fight Candida albicans and Cutibacterium acnes Biofilms: Impact of Dual-Species Conditions

Microbial Warfare on Three Fronts: Mixed Biofilm of Aspergillus fumigatus and Staphylococcus aureus on Primary Cultures of Human Limbo-Corneal Fibroblasts

Occurrence of filamentous fungi in drinking water: their role on fungal-bacterial biofilm formation

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