Negative feedback on the effects of stem cell factor on hematopoiesis is partly mediated through neutral endopeptidase activity on substance P: a combined functional and proteomic study

Joshi, Deval D.; Dang, Anju; Yadav, Prem N.; Qian, Jing; Bandari, Persis; Chen, Kunhua; Donnelly, Robert; Castro, Tammy; Gascón, Pedro; Haider, Ali; Rameshwar, Pranela

doi:10.1182/blood.v98.9.2697

Cited by 48 publications

(47 citation statements)

References 40 publications

(123 reference statements)

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“…Despite the significant role of NEP in reducing Ab levels in brain, little is known about factors that regulate its neural expression. In non-neural cells, various factors have been reported to increase NEP activity and expression, including opioids in monocytes (Wang et al 1998), transforming growth factor b in renal smooth muscle cells (Tharaux et al 1997), phorbol ester in choriocarcinoma cells (Suzuki et al 2002), substance P in bone marrow cells (Joshi et al 2001), and cAMP in endothelial cells (Graf et al 1995). Notably, androgens are known to increase NEP expression in prostate cancer cell lines (Papandreou et al 1998;Shen et al 2000a,b).…”

Section: Discussionmentioning

confidence: 99%

Androgens regulate neprilysin expression: role in reducing β‐amyloid levels

Yao

Nguyen

Rosario

et al. 2008

Journal of Neurochemistry

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Age‐related testosterone depletion in men is a risk factor for Alzheimer’s disease. Prior studies suggest that androgens affect Alzheimer’s disease risk by regulating accumulation of β‐amyloid protein (Aβ) by an undefined mechanism. In this study, we investigated the role of the Aβ‐catabolizing enzyme neprilysin (NEP) in this process. First, we observed that androgens positively regulate neural expression of NEP in adult male rats. Next, we investigated androgen regulatory effects on both NEP expression and Aβ levels using cultured hippocampal neurons and neuronally differentiated rat pheochromocytoma cell 12 with or without androgen receptor (AR). Dihydrotestosterone (DHT) induced a time‐dependent increase in NEP expression. DHT also significantly decreased levels of Aβ in AR‐expressing cells transfected with amyloid precursor protein, but did not affect levels of either full‐length or non‐amyloidogenic, soluble amyloid precursor protein. Importantly, the DHT induced decrease of Aβ was blocked by pharmacological inhibition of NEP. The DHT‐mediated increase in NEP expression and decrease in Aβ levels were (i) not observed in rat pheochromocytoma cell 12 lacking AR and (ii) blocked in AR‐expressing cells by the antagonists, cyproterone acetate and flutamide. Together, these findings suggest that androgen regulation of Aβ involves an AR‐dependent mechanism requiring up‐regulation of the Aβ catabolizing enzyme NEP.

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Section: Discussionmentioning

confidence: 99%

Androgens regulate neprilysin expression: role in reducing β‐amyloid levels

Yao

Nguyen

Rosario

et al. 2008

Journal of Neurochemistry

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“…Northern analyses for steady-state PPT-I mRNA in BM stroma and BCCs were performed as described previously (22). To be sure that adequate RNA was obtained from each cell subset, the coculture was established with 10 to 15 replicates in 25-cm 2 flasks.…”

Section: ϫ2mentioning

confidence: 99%

Bone Marrow Stroma Influences Transforming Growth Factor-β Production in Breast Cancer Cells to Regulate c-myc Activation of the Preprotachykinin-I Gene in Breast Cancer Cells

Moharita²,

Potian³

et al. 2004

Cancer Research

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“…Recently, several other neuropeptides, such as substance P, somatostatin, and calcitonin-gene related peptide, have been reported to have effects on hematopoietic progenitor cells. [45][46][47][48] How VIP regulates hematopoiesis in concert with these neuropeptides should be investigated in the future. Growth-promoting effects of VIP on CD34 þ CB cells M Kawakami et al…”

Section: Cd34mentioning

confidence: 99%

Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+CD38− cells: possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells

et al. 2004

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Primitive hematopoietic progenitor cells such as severe combined immunodeficiency-repopulating cells and long-term culture-initiating cells are enriched in CD34 þ CD38 À cells derived from various stem cell sources. In this study, to elucidate the features of such primitive cells at the molecular level, we tried to isolate genes that were preferentially expressed in umbilical cord blood (CB)-derived CD34 þ CD38À cells by subtractive hybridization. The gene for VPAC1 receptor, a receptor for the neuropeptide vasoactive intestinal peptide (VIP), was thereby isolated and it was shown that this gene was expressed in both CD34 þ CD38 À and CD34 þ CD38þ CB cells and that the expression levels were higher in CD34 þ CD38 À CB cells. Next, we assessed the effects of VIP on the proliferation of CD34 þ CB cells using in vitro culture systems. In serum-free single-cell suspension culture, VIP enhanced clonal growth of CD34 þ CB cells in synergy with FLT3 ligand (FL), stem cell factor (SCF), and thrombopoietin (TPO). In serum-free clonogenic assays, VIP promoted myeloid (colony-forming unit-granulocyte/macrophage (CFU-GM)) and mixed (CFU-Mix) colony formations. Furthermore, in Dextertype long-term cultures, VIP increased colony-forming cells at week 5 of culture. These results suggest that VIP functions as a growth-promoting factor of CB-derived hematopoetic progenitor cells.

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Negative feedback on the effects of stem cell factor on hematopoiesis is partly mediated through neutral endopeptidase activity on substance P: a combined functional and proteomic study

Cited by 48 publications

References 40 publications

Androgens regulate neprilysin expression: role in reducing β‐amyloid levels

Androgens regulate neprilysin expression: role in reducing β‐amyloid levels

Bone Marrow Stroma Influences Transforming Growth Factor-β Production in Breast Cancer Cells to Regulate c-myc Activation of the Preprotachykinin-I Gene in Breast Cancer Cells

Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34+CD38− cells: possible role of VIP as a growth-promoting factor for hematopoietic stem/progenitor cells

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