Negative Effects of Alcohol Intake and Estrogen Deficiency Combination on Osseointegration in a Rat Model

Abstract

Alcohol intake and estrogen deficiency can both affect bone physiology and have shown to have an adverse effect on dental implant therapy. However, the combination of both factors on osseointegration is unknown. The aim of this study was to evaluate osseointegration in rats fed with alcohol and presenting induced estrogen deficiency. Ninety-six female rats were divided according to diet and hormonal condition into 6 groups as follows: group Sh-W: sham (simulated ovariectomy) control, food and water ad libitum; group Sh-Et: sham, food and 20% ethanol solution ad libitum; group Sh-Su: sham, food and sucrose solution controlled to ensure an isocaloric diet in relation to Sh-Et; group Ov-W: ovariectomy, food and water ad libitum; group Ov-Et: ovariectomy, food and 20% ethanol solution ad libitum; and group Ov-Su: ovariectomy, food and sucrose solution controlled to ensure an isocaloric diet as Ov-Et. The groups were subdivided according to time of euthanasia: 30 and 45 days after placement of implants. Implant surgery was performed 1 month after ovariectomy or sham. After euthanasia, the femurs were removed and evaluated by histomorphometry. Groups Ov-Et and Ov-Su showed the lowest percentage of bone-to-implant contact. The combination of alcohol intake and estrogen deficiency, and the combination of estrogen deficiency and reduced ingestion of food can negatively affect osseointegration in rats.

Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

“…In the present study, the Ovx/adlibitum group gained significantly more weight from all the other groups, as reported earlier 21 , which contradicts another study 25 that found no statistically significant differences. The Ovx/ad-libitum group also consumed more food, as reported earlier 21 .…”

“…Animals from all groups gained weight, which was reported previously 24,25 . In the present study, the Ovx/adlibitum group gained significantly more weight from all the other groups, as reported earlier 21 , which contradicts another study 25 that found no statistically significant differences.…”

“…Considering these effects of ovariectomy and reported deleterious effects of alcohol consumption on bone tissues 14,21,25 , important modifications of RANKL, BSP, and OCC staining intensities were anticipated in the Ovx/alcohol group compared to other groups. However, similar to the histological and histomorphometric results, no differences were found between the Ovx/alcohol group and other groups, thus rejecting the study hypothesis.…”

Influence of chronic alcoholism and estrogen deficiency on the immunohistochemical expression of regulatory proteins of the bone resorption process in the periodontium of Wistar rats

“…The dietary aspects of this study, as well the surgical procedures, were based on previously published studies which also analyzed the influence of alcohol consumption (20%) and estrogen deficiency in the periodontium of rats (Alonso, Souza, Balducci, & Rocha, 2016;Marchini et al, 2012Marchini et al, , 2014 or other rat tissues (de Deco et al, 2011;Lodi, Marchini, Santo, Rode, & Rocha, 2016;Salgado, Marchini, Tera, Rocha, & Marchini, 2015). However, it is important to highlight that the present study is not part of the abovementioned studies, as we used different animals and different analyses.…”

Microstruttura e funzione del tessuto osseo. Parte II: carico immediato e biomateriali

“…Il topo non è incluso nella lista; tuttavia, sono numerosi gli studi che lo impiegano come modello [20][21][22][23][24][25][26][27][28] .…”