Negative Cellular Effects of Urban Particulate Matter on Human Keratinocytes Are Mediated by P38 MAPK and NF-κB-dependent Expression of TRPV 1

Kwon, Ki-Won; Park, See‐Hyoung; Han, Byung Hyuk; Oh, Sae Woong; Lee, Seung; Yoo, Ju Ah; Park, Se; Kim, Jangsoon; Kim, Ji; Cho, Jae; Lee, Jongsung

doi:10.3390/ijms19092660

Cited by 16 publications

(43 citation statements)

References 34 publications

(49 reference statements)

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“…In 3D HSE models, a systemic treatment with PM (200 μg/mL) induced alterations in skin morphology and expression levels of structural proteins after a long time exposure (i.e., 48 h up to 6 days) [125,126]. In 3D HEE models, PM usually in concentrations of up to 50 μg/mL was mainly applied topically and short-term exposures (i.e., 24 to 48 h) were performed [128,130,132,133]. PM was shown to affect the physical epidermal barrier in 3D models, as indicated by altered expression of proteins important for epidermal differentiation, such as filaggrin, involucrin, loricrin, and keratins [125,127,129,130,132,133].…”

Section: Physical Barriermentioning

confidence: 99%

“…In 3D HEE models, PM usually in concentrations of up to 50 μg/mL was mainly applied topically and short-term exposures (i.e., 24 to 48 h) were performed [128,130,132,133]. PM was shown to affect the physical epidermal barrier in 3D models, as indicated by altered expression of proteins important for epidermal differentiation, such as filaggrin, involucrin, loricrin, and keratins [125,127,129,130,132,133]. Additionally, markers for proliferation, such as Ki67, 5-bromo-2′-deoxyuridine (BrdU) incorporation, and proliferating cell nuclear antigen (PCNA), were shown to be decreased upon PM exposure [125,130].…”

Section: Physical Barriermentioning

confidence: 99%

“…PM was shown to affect the physical epidermal barrier in 3D models, as indicated by altered expression of proteins important for epidermal differentiation, such as filaggrin, involucrin, loricrin, and keratins [125,127,129,130,132,133]. Additionally, markers for proliferation, such as Ki67, 5-bromo-2′-deoxyuridine (BrdU) incorporation, and proliferating cell nuclear antigen (PCNA), were shown to be decreased upon PM exposure [125,130]. Reduced expression of differentiation and proliferation markers was also shown in 2D models, i.e., HaCaT and NHEK cells [122,125,127,140,141,143,144].…”

Section: Physical Barriermentioning

confidence: 99%

“…This, in turn, initiates the signaling cascade, and upon activation of NF-κB, the p65 subunit translocates to the nucleus, where it regulates the expression of its target genes that are involved in various processes of the inflammatory response [241]. Indeed, PM induces the phosphorylation and nuclear translation of the p65 unit [128,130,136,137,145,169,179,181,231,233]. The PM-induced activation of NF-κB leads to the increased transcription of its target genes and their protein expressions such as IL-1α, IL-1β, IL-6, IL-8, ICAM1, COX2 and TNF-α [124,128,130,133,135,138,139,142,169,173,181,227,229,230,243].…”

Section: Pm-induced Activation Of Nf-κbmentioning

confidence: 99%

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Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Dijkhoff¹,

Drašler²,

Karakoçak³

et al. 2020

Part Fibre Toxicol

106

116

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Background: Air pollution is killing close to 5 million people a year, and harming billions more. Air pollution levels remain extremely high in many parts of the world, and air pollution-associated premature deaths have been reported for urbanized areas, particularly linked to the presence of airborne nano-sized and ultrafine particles. Main text: To date, most of the research studies did focus on the adverse effects of air pollution on the human cardiovascular and respiratory systems. Although the skin is in direct contact with air pollutants, their damaging effects on the skin are still under investigation. Epidemiological data suggested a correlation between exposure to air pollutants and aggravation of symptoms of chronic immunological skin diseases. In this study, a systematic literature review was conducted to understand the current knowledge on the effects of airborne particulate matter on human skin. It aims at providing a deeper understanding of the interactions between air pollutants and skin to further assess their potential risks for human health. Conclusion: Particulate matter was shown to induce a skin barrier dysfunction and provoke the formation of reactive oxygen species through direct and indirect mechanisms, leading to oxidative stress and induced activation of the inflammatory cascade in human skin. Moreover, a positive correlation was reported between extrinsic aging and atopic eczema relative risk with increasing particulate matter exposure.

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Section: Physical Barriermentioning

confidence: 99%

Section: Physical Barriermentioning

confidence: 99%

Section: Physical Barriermentioning

confidence: 99%

Section: Pm-induced Activation Of Nf-κbmentioning

confidence: 99%

See 2 more Smart Citations

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Dijkhoff¹,

Drašler²,

Karakoçak³

et al. 2020

Part Fibre Toxicol

106

116

View full text Add to dashboard Cite

show abstract

“…Upregulation of TRPV1 is mediated by the p38 mitogen-activated protein kinase (MAPK) and NF-jB signaling pathways by urban particulate matter (UPM) resulting into chronic inflammation [234]. Local acidodic microenvironment incudes TRPV1 activation with consequent NF-jB activation with observed lymphangionesis in lymphatic endothelial cells (LECs) [235].…”

Section: Barrett's Reflux and Bile Acidmentioning

confidence: 99%

NF-κB signaling and crosstalk during carcinogenesis

Brücher

Läng

Jamall

2019

4open

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Transcription factors (TFs) are proteins that control the transcription of genetic information from DNA to mRNA by binding to specific DNA sequences either on their own or with other proteins as a complex. TFs thus support or suppress the recruitment of the corresponding RNA polymerase. In general, TFs are classified by structure or function. The TF, Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), is expressed in all cell types and tissues. NF-κB signaling and crosstalk are involved in several steps of carcinogenesis including in sequences involving pathogenic stimulus, chronic inflammation, fibrosis, establishment of its remodeling to the precancerous niche (PCN) and transition of a normal cell to a cancer cell. Triggered by various inflammatory cytokines, NF-κB is activated along with other TFs with subsequent stimulation of cell proliferation and inhibition of apoptosis. The involvement of NF-κB in carcinogenesis provides an opportunity to develop anti-NF-κB therapies. The complexity of these interactions requires that we elucidate those aspects of NF-κB interactions that play a role in carcinogenesis, the sequence of events leading to cancer.

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Anti‐pollutant effect of oleic acid against urban particulate matter is mediated via regulation of AhR‐ and TRPV1‐mediated signaling in vitro

Choi,

Yang,

Kim

et al. 2024

Environmental Toxicology

Self Cite

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Urban Particulate Matter (UPM) induces skin aging and inflammatory responses by regulating skin cells through the transient receptor potential vanilloid 1 (TRPV1). Although oleic acid, an unsaturated free fatty acid (FFA), has some functional activities, its effect on UPM‐induced skin damage has not been elucidated. Here, we investigated signaling pathways on how oleic acid is involved in attenuating UPM induced cell damage. UPM treatment increased XRE‐promoter luciferase activity and increased translocation of AhR to the nucleus, resulting in the upregulation of CYP1A1 gene. However, oleic acid treatment attenuated the UPM effects on AhR signaling. Furthermore, while UPM induced activation of TRPV1 and MAPKs signaling which activated the downstream molecules NFκB and AP‐1, these effects were reduced by cotreatment with oleic acid. UPM‐dependent generation of reactive oxygen species (ROS) and reduction of cellular proliferation were also attenuated by the treatment of oleic acid. These data reveal that cell damage induced by UPM treatment occurs through AhR signaling and TRPV1 activation which in turn activates ERK and JNK, ultimately inducing NFκB and AP‐1 activation. These effects were reduced by the cotreatment of oleic acid on HaCaT cells. These suggest that oleic acid reduces UPM‐induced cell damage through inhibiting both the AhR signaling and activation of TRPV1 and its downstream molecules, leading to a reduction of pro‐inflammatory cytokine and recovery of cell proliferation.

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Negative Cellular Effects of Urban Particulate Matter on Human Keratinocytes Are Mediated by P38 MAPK and NF-κB-dependent Expression of TRPV 1

Cited by 16 publications

References 34 publications

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

NF-κB signaling and crosstalk during carcinogenesis

Anti‐pollutant effect of oleic acid against urban particulate matter is mediated via regulation of AhR‐ and TRPV1‐mediated signaling in vitro

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