Needs, Challenges and Countermeasures of SARS-CoV-2 Surveillance in Cold-Chain Foods and Packaging to Prevent Possible COVID-19 Resurgence: A Perspective from Advanced Detections

Abstract

The pandemic caused by SARS-CoV-2 has a huge impact on the global economy. SARS-CoV-2 could possibly and potentially be transmitted to humans through cold-chain foods and packaging (namely good-to-human), although it mainly depends on a human-to-human route. It is imperative to develop countermeasures to cope with the spread of viruses and fulfil effective surveillance of cold-chain foods and packaging. This review outlined SARS-CoV-2-related cold-chain food incidents and current methods for detecting SARS-CoV-2. Then the needs, challenges and practicable countermeasures for SARS-CoV-2 detection, specifically for cold-chain foods and packaging, were underlined. In fact, currently established detection methods for SARS-CoV-2 are mostly used for humans; thus, these may not be ideally applied to cold-chain foods directly. Therefore, it creates a need to develop novel methods and low-cost, automatic, mini-sized devices specifically for cold-chain foods and packaging. The review intended to draw people’s attention to the possible spread of SARS-CoV-2 with cold-chain foods and proposed perspectives for futuristic cold-chain foods monitoring during the pandemic.

Analysis of professional competency awareness based on visible network graphs

“…Choosing Baidu Consultation search index as the data source is grounded in several reasons. Firstly, as the largest search engine in China, Baidu's search data can effectively mirror the interests and concerns of a vast number of internet users, rendering it highly representative [36,37]. Secondly, the search index transcends being a mere numerical value; it encapsulates a trove of social, economic, and cultural information [38,39], providing both macro and micro perspectives on the awareness of "professional competency."…”

Nano-biosensor for SARS-CoV-2/COVID-19 detection: methods, mechanism and interface design

“…In particular, CRISPR-Cas12a and CRISPR-Cas13a have attracted interest due to their highly accurate gene-editing capabilities and crosscutting activities. 232 CRISPR-Cas-based and Argonaute-based SARS-CoV-2 detection methods are more specific, convenient, and reliable. Cas12 and Cas13 as RNA-directed CRISPR-related proteins can be programmed to detect specific DNA and RNA sequences from pathogens with single-base pair specificity, respectively.…”

“…Due to their unique biocharacters, they can be independently used to detect viruses with excellent sensitivity. The more important aspect is that they can be integrated with some existing methods to suit diverse needs, such as being coupled to fluorimeters, 232 FETS, 237 SERS, 235 and electrochemical biosensors. 226 These combinations have attracted researchers' interest, and have great potential in nucleic acid detection with high specificity and sensitivity.…”