Needleless emulsion electrospinning for the regulated delivery of susceptible proteins

Abstract

In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-ε-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor]. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.

A comparison of high throughput core–shell 2D electrospinning and 3D centrifugal spinning techniques to produce platelet lyophilisate-loaded fibrous scaffolds and their effects on skin cells

“…The emulsion electrospinning results in the formation of bres for long-term delivery of active molecules. 38 In the case of centrifugal spinning, 19 the bres showed a similar release pattern and the mean release time was shorter than in the studies with electrospun bres. [39][40][41] Nevertheless, the amount of the total protein released (Fig.…”

Advances in Electrospun Hybrid Nanofibers for Biomedical Applications

“…Organic solvents may negatively influence bioactivity of bioactive substances; therefore, core-shell electrospinning where hydrophilic polymers such as PVA is in the core is preferred [ 168 , 169 ]. However, other methods, e.g., needleless electrospinning, can also lead to the production of core-shell nanofibers when using an emulsion system for the preparation of nanofibers [ 169 , 170 , 171 ]. On the other hand, water soluble polymers, e.g., PVA, PEO, PEG, gelatine, hyaluronic acid, and collagen are often used as blends or in composite scaffolds [ 172 , 173 , 174 , 175 ].…”

Core–Shell Fibers: Design, Roles, and Controllable Release Strategies in Tissue Engineering and Drug Delivery

“…The aim of the system is to provide dual delivery of GFs, and this was achieved by encapsulating nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) individually in poly(d,l-lactic acid) (PDLLA) and PLGA fibers, respectively by emulsion electrospinning. A current study of needleless emulsion electrospinning also has been reported, which aims to overcome low productivity of conventional emulsion electrospinning [122]. Horseradish peroxidise (HRP) and multiple GFs (transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), and insulin growth factor-1 (IGF-1)) have been successfully incorporated in the core layer of this system.…”

“…Horseradish peroxidise (HRP) and multiple GFs (transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), and insulin growth factor-1 (IGF-1)) have been successfully incorporated in the core layer of this system. In vitro testing revealed that the as-prepared core-shell fibers promote viability and metabolic activity of porcine mesenchymal stem cells (MSCs) [122].…”