Needleless electrospun and centrifugal spun poly-ε-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs

Lukášová, Věra; Buzgo, Matěj; Vocetková, Karolína; Sovková, Věra; Doupnik, Miroslav; Himawan, Erico; Staffa, Andrea; Sedláček, Radek; Chlup, Hynek; Rustichelli, F.; Amler, Evžen; Rampichová, Michala

doi:10.1016/j.msec.2018.12.069

Cited by 26 publications

(18 citation statements)

References 29 publications

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“…Generally, both types of scaffolds mimic the native extracellular matrix (ECM) owing to their porosity, pore interconnection and fibrous structure. However, the electrospun fibrous layer is more planar, forming a rather 2D-like structure, in comparison to the centrifugally spun fluffy 3D-like scaffolds with higher porosity, improving cell penetration that is impeded to some degree in the 2D-like electrospun layers [ 25 , 39 ]. The µCT evaluation showed a higher number of larger pores in the centrifugally spun samples, when compared to the electrospun samples.…”

Section: Discussionmentioning

confidence: 99%

“…According to their results, centrifugally spun fibers were looser, had higher fiber diameter and broader diameter distribution. A study performed on PCL confirmed that a centrifugally spun fibrous scaffold showed higher porosity, pore size and fiber diameter [ 25 ]. It has been shown that the more open morphology of the centrifugally spun samples is a desirable feature in tissue engineering applications, as it fosters cell penetration into the deeper layers of the scaffold and its colonization [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…Their unique properties, such as high volume-to-surface ratio, porous structure, and nanoscale microarchitecture, predetermine them for tissue engineering applications [ 22 ]. The most common large-scale techniques of nanofiber fabrication include electrospinning (using electrostatic forces to produce fibers) and centrifugal spinning (producing fibers using centrifugal forces) [ 23 , 24 , 25 ]. As a postproduction modification, surface adhesion is the simplest method for scaffold functionalization; however, the loading efficiency and release rate may be quite variable and difficult to optimize.…”

Section: Introductionmentioning

confidence: 99%

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A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

et al. 2020

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Vitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. Platelet-rich plasma (PRP) represents a safe and cheap regenerative therapy option, as it delivers a wide spectrum of native growth factors, cytokines and other bioactive molecules. The aim of this study was to develop a simple delivery system to prolong the effects of the bioactive molecules released from platelets. The surface of electrospun and centrifugally spun poly-ε-caprolactone (PCL) fibrous scaffolds was functionalized with various concentrations of platelets; the influence of the morphology of the scaffolds and the concentration of the released platelet-derived bioactive molecules on melanocytes, was then assessed. An almost two-fold increase in the amount of the released bioactive molecules was detected on the centrifugally spun vs. electrospun scaffolds, and a sustained 14-day release of the bioactive molecules was demonstrated. A strong concentration-dependent response of melanocyte to the bioactive molecules was observed; higher concentrations of bioactive molecules resulted in improved metabolic activity and proliferation of melanocytes. This simple system improves melanocyte viability, offers on-site preparation and is suitable for prolonged topical PRP administration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

et al. 2020

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“…In order to deliver the natural mixture of growth factors that are present during the healing procedure we added the PRP into the composite gel. PRP is widely used in regenerative medicine for the healing of tendons, bones or cartilage [ 85 , 86 , 87 , 88 ].…”

Section: Discussionmentioning

confidence: 99%

Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

et al. 2020

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Hydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested; fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µm) and further modified by polyethylene glycol–biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation.

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“…Centrifugal spinning increases the choice of the materials, productivity, and reduces production cost (PADRON et al, 2013;SARKAR et al, 2010). In recent papers, these fibers were used for battery (FLORES et al, 2018;KIM, 2019), tissue engineering (LUKÁŠOVÁ et al, 2019;MAMIDI et al, 2019a), adsorption (KUMMER et al, 2018ROSTAMIAN;FIROUZZARE;IRANDOUST, 2019), colorimetric sensor for food (VALDEZ et al, 2019), fibrous color dosimeter (KINASHI et al, 2018), pharmaceutical application (AKIA et al, 2019MAMIDI et al, 2019b).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Micro and Nanoparticles Filtration Evaluation of Pan Nanofibers Produced by Centrifugal Spinning

Salussoglia¹,

Tanabe²,

Aguiar³

2019

Enci Bio

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Centrifugal spinning has been used in the production of fibers for the most varied application areas. This paper aims to evaluate the filtration performance of substrate, and polyacrylonitrile (PAN) nanofibers covered at the same substrate, produced by Forcespinning (centrifugal spinning equipment). For this, the characteristics of the nanofiber layer and collection efficiency of micro and nanoparticles were investigated. Sodium chloride was used to generate nanoparticles, and hydrated lime was used as micro particles. Filtration experiments were performed with a superficial gas velocity of 4.8 cm/s. The fiber layer has a single layer with large fiber diameter and pore size dispersion. The results showed that the substrate was more permeable and presented the lower pressure drop than the covered substrate. For nanoparticle collection efficiency, the substrate had an increase of around 20%, due to the addition of PAN nanofibers. For micro particle collection efficiency, at the beginning of the test and after 30 minutes of filtration, the substrate increased collection efficiency from 50.5 to 69.6%, while the covered substrate increased from 71.0 to 97.2%.

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Needleless electrospun and centrifugal spun poly-ε-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs

Cited by 26 publications

References 29 publications

A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

Characterization and Micro and Nanoparticles Filtration Evaluation of Pan Nanofibers Produced by Centrifugal Spinning

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