Needle Microinjection: A Brief History

Feramisco, James R.; Perona, Rosario; Lacal, JC

doi:10.1007/978-3-0348-8705-2_1

Cited by 13 publications

(9 citation statements)

References 61 publications

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“…Finally, our microinjector can be applicable to other creatures like insects, zebra fishes, or even mice with low volume at the dose of 1.3 nL. Single cell (e.g., embryo) injection requires nanoliter-scale injection to prevent the rupture of the cell membrane due to high tension [8,9]. Microinjections of dsRNAs for other planarian genes at similar injection volume (e.g., 1.5~100 nL) are also possible to be performed in the future, in particular, for the genes whose functions are related to starvation conditions.…”

Section: Discussionmentioning

confidence: 99%

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Gene Delivery System Using Droplet Injector and Temperature-Controlled Planarian Holder

et al. 2018

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A microinjection system for gene delivery to a planarian was presented with materials widely used by manufacturers. The system consists of a nanoliter droplet generator/injector and a planarian holder. Glass capillary needles were used to consistently generate droplets and to inject droplets into a planarian. The holder provides a low-temperature environment that immobilizes the planarian for injection. Our system was tested and showed successful injections of microbeads and droplets with double-stranded RNA into the planarian. The results demonstrated the capability of our system as an alternative for gene delivery for studying gene functions in planarians or other living objects for regenerative medicine studies.

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Section: Discussionmentioning

confidence: 99%

“…Microinjection is another widely used gene delivery technique for organ cellular/organismal objects [8,9]. It is simple, direct, and efficient.…”

Section: Introductionmentioning

confidence: 99%

Gene Delivery System Using Droplet Injector and Temperature-Controlled Planarian Holder

et al. 2018

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“…A large variety of molecules like dyes, proteins, nucleic acids, drug compounds or toxins can be injected into cells and their activity can be studied [12,13]. Various microinjection methods exist: the most important methods are capillary pressure microinjection, laser beam injection, electroporation, iontophoresis and various endocytosis methods [14 -20].…”

Section: Automation Of Capillary Pressure Microinjectionmentioning

confidence: 99%

Capillary pressure microinjection of living adherent cells: challenges in automation

Kallio¹,

Kuncová-Kallio²

2006

Journal of Micromechatronics

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This paper is divided into two parts. The first part describes the current status and the general challenges of developing automatic microrobotics systems for microinjection of adherent mammalian cells. The discussion covers applications and the review and challenges of the components of a capillary pressure microinjection system: a micromanipulator, a microinjector, a microcapillary, a vision system and an environment control system. The second part of the paper describes the research performed on the automatic capillary pressure microinjection at Tampere University of Technology. The advanced microinjection system includes two micromanipulators, a microinjector, a vision system and a control system. The control system comprises motion control schemes for the micromanipulators to accurately position a microcapillary, to precisely penetrate a cell membrane and to deliver information on the injection to the operator. A novel injection guidance system, being part of the control system, comprises an impedance measurement device and a user interface which provide information on the detection of the capillary-membrane contact, capillary clogging and capillary breakage. Results show a remarkable increase of the injection success from 40 to 65% when the injection guidance system is used.

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“…Single cell transfection techniques have limited transfection efficiency, dosage control, cell viability, and ease of fabrication 7 . For instance, microinjection, which is the most common transfection technique, damages cells due to the large microinjector needle diameters of 0.5–5 μm 8 and the needles tend to clog before injection 9 . Other single cell transfection techniques such as opto-poration lack controlled dosage delivery and need optimization of parameters for each cell type 10 11 .…”

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confidence: 99%