Needle-Free Jet Injection of a Mixture of Japanese Encephalitis DNA and Protein Vaccines: A Strategy to Effectively Enhance Immunogenicity of the DNA Vaccine in a Murine Model

Imoto, Jun-ichi; Konishi, Eiji

doi:10.1089/vim.2005.18.205

Cited by 33 publications

(14 citation statements)

References 30 publications

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“…The protein component was the driving factor for elicitation of JE-specific NAbs when administered as a vaccine mixture with DNA [39] and as a DNA prime–protein boost vaccine [36]. Our findings also show that the protein component of the vaccine has a stronger influence on antibody specificity with higher binding and neutralizing antibody responses against the envelope cognate to the protein component.…”

Section: Discussionmentioning

confidence: 66%

Improvement of antibody responses by HIV envelope DNA and protein co-immunization

Pissani

Malherbe

Schuman³

et al. 2014

Vaccine

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Background Developing HIV Envelope (Env) vaccine components that elicit durable and protective antibody responses is an urgent priority, given the results from the RV144 trial. Optimization of both the immunogens and vaccination strategies will be needed to generate potent, durable antibodies. Due to the diversity of HIV, an effective Env-based vaccine will most likely require an extensive coverage of antigenic variants. A vaccine co-delivering Env immunogens as DNA and protein components could provide such coverage. Here, we examine a DNA and protein co-immunization strategy by characterizing the antibody responses and evaluating the relative contribution of each vaccine component. Method We co-immunized rabbits with representative subtype A or B HIV gp160 plasmid DNA plus Env gp140 trimeric glycoprotein and compared the responses to those obtained with either glycoprotein alone or glycoprotein in combination with empty vector. Results DNA and glycoprotein co-immunization was superior to immunization with glycoprotein alone by enhancing antibody kinetics, magnitude, avidity, and neutralizing potency. Importantly, the empty DNA vector did not contribute to these responses. Humoral responses elicited by mismatched DNA and protein components were comparable or higher than the responses produced by the matched vaccines. Conclusion Our data show that co-delivering DNA and protein can augment antibodies to Env. The rate and magnitude of immune responses suggest that this approach has the potential to streamline vaccine regimens by inducing higher antibody responses using fewer vaccinations, an advantage for a successful HIV vaccine design.

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Section: Discussionmentioning

confidence: 66%

Improvement of antibody responses by HIV envelope DNA and protein co-immunization

Pissani

Malherbe

Schuman³

et al. 2014

Vaccine

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“…Coimmunization with protein (EPs) and gene (DNA) vaccines is an effective strategy for enhancing their immunogenicity. It has been demonstrated that the ability of one type of vaccine to induce neutralizing antibodies was synergistically increased by simultaneous immunization with another type of vaccine, allowing reductions in the doses of both vaccines and consequently reducing the cost (18,19,22). In this study, mice immunized twice with EPs obtained from transfected Sf9 cells in combination with DNA vaccines produced neutralizing antibodies, and the antibody titers were comparable to those elicited by EPs derived from mammalian cells, indicating that the immunogenicities of EPs derived from insect and mammalian cells are equivalent.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Extracellular Subviral Particles of Dengue Virus Type 2 and Japanese Encephalitis Virus Produced bySpodoptera frugiperdaCells for Use as Vaccine and Diagnostic Antigens

Kuwahara

Konishi

2010

Clin Vaccine Immunol

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“…The transcutaneous microneedle has the ability to bypass the stratum corneum layer of the skin, thus reaching Langerhans cells-the APCs of the skin. Jet-injection mechanical devices deliver DNA vaccines into the viable epidermis and increased efficacy in the prevention and/or therapy of infectious diseases, allergic disorders and cancer (Chen et al 2002;Imoto and Konishi 2005;Roberts et al 2005). The tattooperforating needle device has been used to puncture the skin and transfer DNA into skin-associated cells.…”

Section: Improving Immunogenicity By Using Next-generation Delivery Smentioning

confidence: 99%

Nanotechnological Approaches for Genetic Immunization

2013

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Genetic immunization is one of the important findings that provide multifaceted immunological response against infectious diseases. With the advent of r-DNA technology, it is possible to construct vector with immunologically active genes against specific pathogens. Nevertheless, site-specific delivery of constructed genetic material is an important contributory factor for eliciting specific cellular and humoral immune response. Nanotechnology has demonstrated immense potential for the site-specific delivery of biomolecules. Several polymeric and lipidic nanocarriers have been utilized for the delivery of genetic materials. These systems seem to have better compatibility, low toxicity, economical and capable to delivering biomolecules to intracellular site for the better expression of desired antigens. Further, surface engineering of nanocarriers and targeting approaches have an ability to offer better presentation of antigenic material to immunological cells. This chapter gives an overview of existing and emerging nanotechnological approaches for the delivery of genetic materials.

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Needle-Free Jet Injection of a Mixture of Japanese Encephalitis DNA and Protein Vaccines: A Strategy to Effectively Enhance Immunogenicity of the DNA Vaccine in a Murine Model

Cited by 33 publications

References 30 publications

Improvement of antibody responses by HIV envelope DNA and protein co-immunization

Improvement of antibody responses by HIV envelope DNA and protein co-immunization

Evaluation of Extracellular Subviral Particles of Dengue Virus Type 2 and Japanese Encephalitis Virus Produced bySpodoptera frugiperdaCells for Use as Vaccine and Diagnostic Antigens

Nanotechnological Approaches for Genetic Immunization

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