Nectin stabilization at adherens junctions is counteracted by Rab5a-dependent endocytosis

Cervero, Pasquale; Vrenken, Kirsten S.; Klose, Matthias; Rehm, Kerstin; Linder, Stefan

doi:10.1016/j.ejcb.2021.151184

Cited by 2 publications

(1 citation statement)

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“…Biotinylation was performed according to Cervero et al (2021) , with a cleavable version of biotin (sulfo-NHS-SS-Biotin, A8005; APExBIO) for 30 min on ice. Cells were subsequently washed with ice-cold DPBS (++) and unbound biotin was quenched by washing cells three times for 10 min with ice-cold glycine containing quenching solution (100 mM glycine in DPBS (++); pH: 7.7).…”

Section: Methodsmentioning

confidence: 99%

KIF16B drives MT1-MMP recycling in macrophages and promotes co-invasion of cancer cells

Hey,

Wiesner,

Barcelona

et al. 2023

Life Sci. Alliance

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The matrix metalloproteinase MT1-MMP is a central effector of cellular proteolysis. Accordingly, regulation of the surface-localized pool of MT1-MMP is crucial for cell migration and invasion. Here, we identify the superprocessive kinesin KIF16B as a major driver of fast recycling of MT1-MMP to the surface of primary human macrophages. KIF16B associates with MT1-MMP on Rab14-positive vesicles, and its depletion results in strongly reduced MT1-MMP surface levels, as shown by microscopical, biochemical, and cell-sorting approaches. As a consequence, KIF16B-depleted macrophages exhibit strongly reduced matrix degradation and invasion. We further identify the cargo-binding C-terminus of KIF16B as a critical element of MT1-MMP transport, as its overexpression uncouples MT1-MMP vesicles from the endogenous motor, thus leading to a reduction of surface-associated MT1-MMP and to reduced matrix degradation and invasion. Importantly, depletion of KIF16B in primary macrophages also reduces the co-invasion of cancer cells from tumor spheroids, pointing to the KIF16B-driven recycling pathway in macrophages as an important regulatory element of the tumor microenvironment.

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Section: Methodsmentioning

confidence: 99%