Nectar bacteria affect life history of a generalist aphid parasitoid by altering nectar chemistry

Lenaerts, Marijke; Goelen, Tim; Paulussen, Caroline; Herrera‐Malaver, Beatriz; Steensels, Jan; Ende, Wim Van den; Verstrepen, Kevin J.; Wäckers, Félix; Jacquemyn, Hans; Lievens, Bart

doi:10.1111/1365-2435.12933

Cited by 40 publications

(41 citation statements)

References 58 publications

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“…Similar results were reported by Lenaerts et al. (), who also detected some hetero‐oligosaccharides that contained fructosyl‐fructose linkages and that were not found in sterile nectar.…”

Section: Discussionsupporting

confidence: 90%

“…These molecules are known to have a prebiotic effect on animals (Peshev and Van den Ende 2014). Similar results were reported by Lenaerts et al (2017), who also detected some hetero-oligosaccharides that contained fructosyl-fructose linkages and that were not found in sterile nectar.…”

Section: Yeast Modifications On Nectar Chemistrysupporting

confidence: 85%

“…All experiments were replicated twice, yielding a total of 36 samples (6 yeast species 9 2 colonies per yeast species 9 3 samples per week, treatment T1). High-performance liquid chromatography (HPAEC-PAD) was used to quantify the concentration of 10 sugars (glucose, fructose, sucrose, 1-kestotriose, maltose, inulobiose, 6-kestotriose, 6G-kestotriose [neokestose], nystose, and maltotriose) and 18 amino acids (aspartic acid, glutamic acid, asparagine, serine, glutamine, histidine, glycine, threonine, arginine, alanine, ornithine, tyrosine, lysine, valine, methionine, phenylalanine, iso-leucine, and leucine) following protocols outlined in Lenaerts et al (2017). Samples for HPAEC-PAD were taken by collecting 100 lL of the treatment solution from the feeder; afterward the samples were kept at À80°C until processing.…”

Section: Changes In Nectar Chemistrymentioning

confidence: 99%

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The impact of yeast presence in nectar on bumble bee behavior and fitness

Pozo

Kemenade

Oystaeyen³

et al. 2019

Ecological Monographs

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The presence of yeasts in pollen and floral nectar is rather the norm than the exception. Due to the metabolic activities of yeasts, sugar and amino acid composition of nectar often drastically change and may negatively impact the nutritional value of nectar for pollinators and hence insect fitness. On the other hand, the presence of yeasts in floral nectar may also increase its nutritional value due to yeast's probiotic effect and the release of yeast's metabolites. In this study, we investigated whether the presence of defined flower‐ and insect‐associated yeasts affected individual and colony fitness of the bumble bee pollinator Bombus terrestris. Specifically, we tested whether the presence of yeasts in nectar affected bumble bee foraging behavior and nectar consumption, individual growth and colony development, larval and queen mortality, and mating success. Quantitative analyses of sugar and amino acid profiles showed that nectar yeasts significantly affected the chemical composition of nectar. However, dual‐choice experiments indicated that yeast inoculation did not significantly affect foraging behavior or consumption rates. Nest development, on the other hand, was significantly affected by the presence of yeasts, but effects largely depended on species identity, with Candida bombiphila, Metschnikowia gruessii, and Rhodotorula mucilaginosa having the largest positive impact on colony growth. Interestingly, the effects at the colony level were more pronounced than at the individual level. In vitro growth tests further showed that yeasts have the potential to suppress the growth of the bumble bee gut pathogen Crithidia bombi. Overall, these results demonstrate that nectar‐inhabiting yeasts can have diverse effects on bumble bee fitness and therefore may mediate plant–pollinator mutualisms.

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Section: Discussionsupporting

confidence: 90%

Section: Yeast Modifications On Nectar Chemistrysupporting

confidence: 85%

Section: Changes In Nectar Chemistrymentioning

confidence: 99%

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The impact of yeast presence in nectar on bumble bee behavior and fitness

Pozo

Kemenade

Oystaeyen³

et al. 2019

Ecological Monographs

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“…) have been previously isolated from floral nectar or floral tissue, including Enterococcus (OTU 278; Álvarez‐Pérez and Herrera ) , Lactococcus (OTU 19; Lenaerts et al. ) , and Leuconostoc (OTU 37; Álvarez‐Pérez and Herrera , Samuni‐Blank et al. ).…”

Section: Discussionmentioning

confidence: 99%

Legitimate visitors and nectar robbers of Aquilegia formosa have different effects on nectar bacterial communities

2018

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Metacommunity structure is strongly influenced by dispersal between habitat patches. Dispersal mode (e.g., active or passively via vector, wind, or water) is recognized to influence metacommunity dynamics, but it is not well understood how within-mode heterogeneity impacts dispersal and community assembly, particularly for microbial communities. Microbes often rely on flower visitors for dispersal among short-lived floral nectar habitats, but it is unclear whether flower visitor guilds (e.g., legitimate visitors vs. larcenists) differentially influence nectar microbial diversity and community structure. We surveyed the community of legitimate nectar foragers and nectar robbers, which damage flowers to obtain floral rewards, of Aquilegia formosa. Then, we evaluated how manipulating access by legitimate nectar foragers, primary nectar robbers, and/or secondary nectar robbers influenced the diversity, species composition, and beta diversity of nectar bacteria within individual flowers. A taxonomically diverse insect community visited A. formosa, and visitors differentially influenced nectar bacterial community structure at within-flower (local) and among-flower (regional) scales. When legitimate nectar foragers were allowed to access A. formosa, we observed an increase in bacterial diversity and changes in bacterial species composition such that common nectar bacteria had higher relative abundances. In contrast, effects of natural and simulated robbing had little effect on bacterial alpha diversity, but simulated robbing decreased the relative abundance of common nectar bacteria, and natural nectar robbing events reduced beta diversity of nectar bacteria. This work highlights the importance of visitor identity on microbial diversity and species composition in flowers, and, more broadly, suggests that vectors can differentially influence metacommunity structure.

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“…We used three colonies of the generalist bumble bee Bombus impatiens ( influence nectar chemistry and scent [21]. 73 74…”

Section: Study System 67mentioning

confidence: 99%

Microbial metabolites mediate bumble bee attraction and feeding

Schaeffer

Rering

Maalouf

et al. 2019

Preprint

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16Animals such as bumble bees use chemosensory cues to localize and evaluate essential 17 resources. Increasingly, it is recognized that microbes can alter the quality of foraged resources 18 and produce metabolites that act as foraging cues. The distinct nature of these sensory cues 19 however and their use in animal foraging remain poorly understood. Here, we test the hypothesis 20 that species of nectar-inhabiting microbes differentially influence pollinator attraction and 21 feeding via microbial metabolites in nectar. We examined electrophysiological potential of 22 32

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Nectar bacteria affect life history of a generalist aphid parasitoid by altering nectar chemistry

Cited by 40 publications

References 58 publications

The impact of yeast presence in nectar on bumble bee behavior and fitness

The impact of yeast presence in nectar on bumble bee behavior and fitness

Legitimate visitors and nectar robbers of Aquilegia formosa have different effects on nectar bacterial communities

Microbial metabolites mediate bumble bee attraction and feeding

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