NecroX-5 Can Suppress Melanoma Metastasis by Reducing the Expression of Rho-Family GTPases

Moon, Gue-Tae; Lee, Ji Hyun; Jeong, Soon‐Jong; Jin, Songwan; Park, Young-Min

doi:10.3390/jcm10132790

Cited by 1 publication

(2 citation statements)

References 33 publications

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“…CCG‐1423 (APExBIO) selectively inhibits SRF‐mediated transcription of Rho signalling pathway activation 21 . Y‐27632 (AbMole Bioscience) is a pharmacologically specific inhibitor of ROCK 22 .…”

Section: Methodsmentioning

confidence: 99%

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RhoA/ROCK1 regulates the mitochondrial dysfunction through Drp1 induced by Porphyromonas gingivalis in endothelial cells

Qin

Ying

Yin

et al. 2023

J Cellular Molecular Medi

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Porphyromonas gingivalis (P. gingivalis) is a pivotal pathogen of periodontitis. Our previous studies have confirmed that mitochondrial dysfunction in the endothelial cells caused by P. gingivalis was dependent on Drp1, which may be the mechanism of P. gingivalis causing endothelial dysfunction. Nevertheless, the signalling pathway induced the mitochondrial dysfunction remains unclear. The purpose of this study was to investigate the role of the RhoA/ROCK1 pathway in regulating mitochondrial dysfunction caused by P. gingivalis. P. gingivalis was used to infect EA.hy926 cells (endothelial cells). The expression and activation of RhoA and ROCK1 were assessed by western blotting and pull‐down assay. The morphology of mitochondria was observed by mitochondrial staining and transmission electron microscopy. Mitochondrial function was measured by ATP content, mitochondrial DNA and mitochondrial permeability transition pore openness. The phosphorylation and translocation of Drp1 were evaluated using western blotting and immunofluorescence. The role of the RhoA/ROCK1 pathway in mitochondrial dysfunction was investigated using RhoA and ROCK1 inhibitors. The activation of RhoA/ROCK1 pathway and mitochondrial dysfunction were observed in P. gingivalis‐infected endothelial cells. Furthermore, RhoA or ROCK1 inhibitors partly prevented mitochondrial dysfunction caused by P. gingivalis. The increased phosphorylation and mitochondrial translocation of Drp1 induced by P. gingivalis were both blocked by RhoA and ROCK1 inhibitors. In conclusion, we demonstrate that the RhoA/ROCK1 pathway was involved in mitochondrial dysfunction caused by P. gingivalis by regulating the phosphorylation and mitochondrial translocation of Drp1. Our research illuminated a possible new mechanism by which P. gingivalis promotes endothelial dysfunction.

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Section: Methodsmentioning

confidence: 99%

“…CCG‐1423 (APExBIO) selectively inhibits SRF‐mediated transcription of Rho signalling pathway activation. 21 Y‐27632 (AbMole Bioscience) is a pharmacologically specific inhibitor of ROCK. 22 They were used to determine the regulatory role of RhoA/ROCK1 pathway in P. gingivalis infection cells.…”

Section: Methodsmentioning

confidence: 99%