Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea canephora)

This study aimed to evaluate the efficacy of Apis dorsata honey on calcium levels in the mandibular bone of Wistar rats after ovariohysterectomy as an animal osteoporosis model for treating cases of osteoporosis. This study used 20 female rats, which were divided into five treatment groups with four replications. SHAM was the negative control group; OH was the ovariohysterectomized group without treatment as a positive control; the AD1, AD2, and AD3 groups were ovariohysterectomized rats with A. dorsata honey at doses of 1 g/kg bw, 2 g/kg bw, and 4 g/kg bw for 84 days. After 84 days of treatment, the left mandibular bones of the rats were collected, weighed, and stored in 10% neutral buffered formalin. Calcium levels were calculated using proximate analysis. The results showed a decrease in bone calcium levels in the ovariohysterectomy group, the highest results were in the SHAM group, and the lowest results were in the AD3 group. It can be concluded that the administration of A. dorsata honey could not maintain the calcium level of the mandibular bone in the ovariohysterectomized Wistar rats.

Section: Resultsmentioning

confidence: 99%

The Effect of Apis dorsata Honey Treatment on Calcium Levels of Mandibular Bone in Ovariohysterectomized Wistar Rats

Rohmah,

Yudaniayanti,

Primarizky

et al. 2023

“…Flavonoids fight off free radicals by donating their electrons to molecules that have unpaired electrons such as free radicals while remaining stable and also making the reactive molecules achieve stability hence, flavonoids play an important role in inhibiting the increase of free radicals in the body (Weliyani et al, 2021;Listyorini et al, 2021). The phenolic compounds of honey play an important role in donating electrons and hydrogen atoms from the hydroxyl groups of phenolics and function to stabilize free radical compounds (Al-Zuhroh et al, 2021). This compound also functions as a scavenger of oxygen and helps regenerate endogenous antioxidants.…”

Section: Jurnal Medik Veterinermentioning

confidence: 99%

Protective Effects of Apis Dorsata Honey Supplementation on Kidney Histopathology in Mice with Monosodium Glutamate Exposure

Rao,

Widjiati,

Lokapirnasari

et al. 2023

This study was conducted to investigate the protective effects of Apis dorsata honey on the histopathological changes of the kidney in mice exposed to monosodium glutamate. This study used 25 male mice as the experimental animal which were divided into five groups with five replication, respectively i.e. (C-) was administered basal diet, (C+) was administered 4 mg/g BW MSG, (T1) was administered A. dorsata honey of 53.82 mg/20g BW followed by MSG 4 mg/g BW, (T2) was administered A. dorsata honey of 107.64 mg/20g BW followed by MSG 4 mg/g BW, and (T3) was administered A. dorsata honey of 161.46 g/20g BW followed by MSG 4 mg/g BW. All treatments were carried out orally for 52 days. This study was observed tubular epithelial hydropic degeneration, epithelial necrosis, and glomerular necrosis. Data were analyzed using Kruskal-Wallis test followed by the Mann-Whitney test. As a result, for the epithelial and glomerular necrosis variables, the T3 group showed significant results when compared with C+, T1, and T2 groups. Moreover, T3 was not significantly different from the C- group. It can be concluded that A. dorsata honey in T3 group could protect mice kidneys from the damaging effect of MSG.

“…According to literature and study articles, several essential oils can help in reducing anxiety disorders and as a result, the embodied events that they may cause (Al-Zuhroh et al, 2021). Reported data claim that Piper methysticum and Bacopa Monniera are associated with anxiolytic activity in humans.…”

Section: Groupsmentioning

confidence: 99%

Essential Oil of Patchouli Inhalation Exhibits An Anxiolytic-Like Effect in Mice: Elevated Plus-Maze Test

Johansyah,

Hestianah,

Rimayanti

et al. 2023

Anxiety disorder is one of the important public health problems. This study aimed to find out whether patchouli (Pogostemon cablin (Blanco) Benth.) essential oil (PEO) has an anxiolytic-like effect in mice. A total of 20 mice were divided into five treatment groups i.e. (C1) was treated with 1 mg/kg of saline intraperitoneally; (C2) was treated with 1 mg/kg of diazepam intraperitoneally; (T1) 1% PEO inhalation, (T2) 2.5% PEO inhalation, and (T3) 5% PEO inhalation. All experimental animals were put in the Elevated Plus-Maze (EPM), observed for five minutes, and investigated the following parameters i.e. the total number of squares that are transverse in the open arms (STO), the number of visits to the open arms (VOA), the number of visits to the closed arms (VCA), the time spent in the open arms (TOA), and the time spent in the closed arms (TCA). In results, T1 and T2 groups had a minor impact on the VOA. T1 group also had little effect on TOA. Closed-arm activities (VCA and TCA) were unaffected by any of the treatments. The conclusion was revealed that T1 or less of PEO inhalation can potentially provide anxiolytic-like effects in mice.