NECROPSY: USEFUL TOOL TO STUDY THE LIFE HABITS AND THE PARASITISM BY Dioctophyme renale IN MANED WOLF (Chrysocyonbrachyurus), BRAZIL

Abstract

The maned wolf is a wild canid found in cerrado areas or in open fields in Brazil. A large number of these animals host parasites such as Dioctophyme renale, Dipylidium sp., Toxocara sp., Ancylostoma caninum and coccidia which may lead to death due to stressing situations. Although scarce, epidemiological studies of wild canids have cited that parasitic diseases can be the first causes of maned wolf mortality. Therewith, necropsy technique can be a useful tool to understand important characteristics these wild animals with risk of extinction. In this work, a case of a maned wolf parasitized by D. renale is reported. A young male maned Wolf was found dead and in rigor mortis in a road at Pindamonhangaba Municipality, São Paulo, Brazil. The animal underwent necropsy in the Veterinary Hospital of Universidade Paulista – UNIP, São Jose dos Campos Campus, São Paulo, Brazil. During necropsy, intense epistasis, oral cavity bleeding, hemothorax and hemoperitoneum were observed, suggesting that the cause of death of this maned wolf was traumatism, probably due to road killing. In the stomach, several rats and Diptera larvae were found partially digested. The Diptera larvae detection suggests a meat‐based diet in a state of putrefaction what can be explained by diet restriction in urban areas. The right kidney showed renal parenchyma atrophy, bloody content and three D. renale specimens: one female, one male and one larva. The bloody content present in the kidney was analyzed under an optical microscope. The identified eggs were bioperculate, elliptical, brown and had a thick layer, characteristic of D. renale, confirming the literature findings. The necropsy showed be an important understanding tool of life habits of wild animals that have undergone many changes with increasing urbanization.

Identificación y caracterización de proteínas específicas del nematode parásito Dioctophyme renale

“…La proteína con la que encontramos similitudes de secuencia de aminoácidos con P44 es una proteína inusual, las policisteína y cola de histidina (PCHTPs) de especies de los géneros Trichinella y Trichuris (Figura 16.2). Radoslavov y col. [ 29] encontraron que la proteína de T. spiralis Ts-PCHTP interactúa con DAUDA, pero no de manera convincente. Eso podría deberse a un plegamiento incorrecto de la forma recombinante de la proteína que se usó dado su número inusualmente alto de cisteínas, lo que podría conducir a una alta proporción de proteínas unidas incorrectamente a los disulfuros.…”

“…Los huevos se dispersan a través de la orina en el medio ambiente, donde son ingeridos por un anélido (generalmente un oligoqueto) en el que las larvas del parásito se desarrollan hasta la etapa L3(28)1. Si los anélidos infectados no son consumidos directamente por un huésped definitivo, las larvas pueden persistir en hospedadores paraténicos como ranas, peces y roedores, e incluso tortugas de agua dulce (29).…”