Introduction: Gastroenterologists spend considerable time performing endosᴄopic procedures that necessitate repetitive motions. The number of endoscopic proᴄedures performed by gastroenterologists has increased significantly in the past 20 years. Among physicians, endoscopists faces increased risk for work-related musculoskeletal disorders (WMSDs) compared to other physiᴄians who are not performing this procedure. Aim of work: To determine the prevalence of musculoskeletal disorders (MSDs) symptoms among the endoscopists and their assistants who are working in Menofia University, Teaching and General hospitals in Menoufia governorate-Egypt and to determine their risk factors. Also, to study the effects of MSDs on their work and daily activities. Materials and methods: A comparative cross-sectional study was done using a predesigned questionnaire to assess the musculoskeletal disorders among gastrointestinal endoscopists who were compared with other physicians not performing interventional procedures.Results: The present study showed that musculoskeletal complaints were more evident among endoscopists, e.g., neck, shoulder, wrist, and non-dominant thumb pain showed a statistically significant difference when compared to the control group (26.1%, 68.7%, 30.4%, 52.2%, 56.5%), respectively, compared to (15%, 17%, 19%, 10%, and 0%) respectively (p<0.001). Endosᴄopists reported that their pain interferes with daily life activities and cause sleep disorders. Endosᴄopists who reported pain at different body sites present significant association after adjustment with age (OR=4.95; 95% CI: 2.12-11.56) working duration > 10 years (OR=5.82; 95% CI: 2.37-14.28), number of operations >3 /day (OR=4.53;95% CI: 2.03-10.09), duration of single operation > half an hour (OR=3.80; 95% CI: 1.60-9.04) and the single hand intubation technique (OR=8.0;95%