Necessity of Meningococcal γ-Glutamyl Aminopeptidase for
            <i>Neisseria meningitidis</i>
            Growth in Rat Cerebrospinal Fluid (CSF) and CSF-Like Medium

Takahashi, Hideyuki; Hirose, Kenji; Watanabe, Haruo

doi:10.1128/jb.186.1.244-247.2004

Cited by 29 publications

(38 citation statements)

References 28 publications

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“…To confirm the effect of ⌬lptA N. meningitidis, the wild-type lptA gene was ectopically complemented in ggt of the ⌬lptA mutant. The insertion at the ggt locus does not affect adhesive activity (35). The LptA protein was sufficiently expressed in the lptA ϩ -complemented ⌬lptA mutant HT1277 but not in the isogenic strain HT1278, which was not complemented (Fig.…”

Section: Resultsmentioning

confidence: 99%

Modification of Lipooligosaccharide with Phosphoethanolamine by LptA in Neisseria meningitidis Enhances Meningococcal Adhesion to Human Endothelial and Epithelial Cells

et al. 2008

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Section: Resultsmentioning

confidence: 99%

Modification of Lipooligosaccharide with Phosphoethanolamine by LptA in Neisseria meningitidis Enhances Meningococcal Adhesion to Human Endothelial and Epithelial Cells

et al. 2008

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“…This key enzyme was previously reported to act as an important regulator for intracellular homeostasis of oxidative stress, osmotic stress, and utilizing nutrients and facilitating growth in cysteine-limited habitats in other bacteria [34]–[36], hence its presence may influence capability of the strains in host survival. On the other hand, SH0165 genome encodes two enzymes involved in LPS biosynthesis that are absent in ZJ0906 – waaB and waaU .…”

Section: Resultsmentioning

confidence: 99%

Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China

Kwok

Jiang

et al. 2013

PLoS ONE

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Haemophilus parasuis is the etiological agent of Glässer's disease in pigs and 15 standard serovars were identified. The widespread disease causes great economic loss in the swine industry worldwide. Aiming to investigate the differences in genome composition and functions among various strains, a highly virulent strain ZJ0906 of H. parasuis serovar 12 from China was analyzed and compared with serovar 5 SH0165. Strain ZJ0906 genome is 2,324,740 base pairs with 40.06% genomic GC content. It contains 2,484 open reading frames (ORF) predicted by Glimmer 3.02, of which 2,352 (∼94.7%) were annotated by NCBI nr blast, 1,745 by COG database and 1,829 by KEGG database. 109 potential virulence factors were annotated in strain ZJ0906 and 3 of which are potentially related to antibiotic resistance. Strain ZJ0906 genome is ∼55 kilobases longer than SH0165 genome, with an extra 211 predicted ORFs. VFDB, ARDB, and PAIDB blast searches showed that ZJ0906 and SH0165 shared a nearly identical panel of potential virulence factors, drug resistant genes and four PAI-like regions which showed high homology to Enterococcus, Escherichia and Salmonella. Synteny analysis showed that gene rearrangements are frequent between the two strains, which may lead to variations in pathogenicity and cross-protection among serovars. KEGG pathway analyses showed strain ZJ0906 shared similar metabolic pathways to strain SH0165. Molecular identification of these genomic elements and potential virulence factors pave the way to the better understanding of mechanisms underlying metabolic capabilities and pathogenicity of H. parasuis and prospective vaccine targets besides the widely used method of inactivated bacteria.

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“…N. meningitidis causes meningitis, which is due to the meningococcal invasion into the human central nervous system, including cerebrospinal fluid (CSF) [36-38]. It has been shown that meningococcal GGT has a physiological function of acquiring cysteine from environmental γ-glutamyl-cysteinyl peptides under cysteine-limited environments such as the CSF [39]. Almost all (98.8 %) meningococcal isolates from humans are positive for GGT activity [40].…”

Section: Discussionmentioning

confidence: 99%

A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent

Takahashi

Watanabe

2005

BMC Microbiol

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BackgroundIt has been speculated that the γ-glutamyl transpeptidase (ggt) gene is present only in Neisseria meningitidis and not among related species such as Neisseria gonorrhoeae and Neisseria lactamica, because N. meningitidis is the only bacterium with GGT activity. However, nucleotide sequences highly homologous to the meningococcal ggt gene were found in the genomes of N. gonorrhoeae isolates.ResultsThe gonococcal homologue (ggt gonococcal homologue; ggh) was analyzed. The nucleotide sequence of the ggh gene was approximately 95 % identical to that of the meningococcal ggt gene. An open reading frame in the ggh gene was disrupted by an ochre mutation and frameshift mutations induced by a 7-base deletion, but the amino acid sequences deduced from the artificially corrected ggh nucleotide sequences were approximately 97 % identical to that of the meningococcal ggt gene. The analyses of the sequences flanking the ggt and ggh genes revealed that both genes were localized in a common DNA region containing the fbp-ggt (or ggh)-glyA-opcA-dedA-abcZ gene cluster. The expression of the ggh RNA could be detected by dot blot, RT-PCR and primer extension analyses. Moreover, the truncated form of ggh-translational product was also found in some of the gonococcal isolates.ConclusionThis study has shown that the gonococcal ggh gene is a pseudogene of the meningococcal ggt gene, which can also be designated as Ψggt. The gonococcal ggh (Ψggt) gene is the first identified bacterial pseudogene that is transcriptionally active but phenotypically silent.

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Necessity of Meningococcal γ-Glutamyl Aminopeptidase for Neisseria meningitidis Growth in Rat Cerebrospinal Fluid (CSF) and CSF-Like Medium

Cited by 29 publications

References 28 publications

Modification of Lipooligosaccharide with Phosphoethanolamine by LptA in Neisseria meningitidis Enhances Meningococcal Adhesion to Human Endothelial and Epithelial Cells

Modification of Lipooligosaccharide with Phosphoethanolamine by LptA in Neisseria meningitidis Enhances Meningococcal Adhesion to Human Endothelial and Epithelial Cells

Complete Genome Analysis of a Haemophilus parasuis Serovar 12 Strain from China

A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent

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