2021
DOI: 10.1021/acs.biochem.1c00445
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Necessity of Flanking Repeats R1′ and R8′ of Human Pumilio1 Protein for RNA Binding

Abstract: Human Pumilio (hPUM) is a structurally well-analyzed RNA-binding protein that has been used recently for artificial RNA binding. Structural analysis revealed that amino acids at positions 12, 13, and 16 in the repeats from R1 to R8 each contact one specific RNA base in the eight-nucleotide RNA target. The functions of the N- and C-terminal flanking repeats R1′ and R8′, however, remain unclear. Here, we report how the repeats contribute to overall RNA binding. We first prepared three mutants in which R1′ and/or… Show more

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