Abstract:Human
Pumilio (hPUM) is a structurally well-analyzed RNA-binding
protein that has been used recently for artificial RNA binding. Structural
analysis revealed that amino acids at positions 12, 13, and 16 in
the repeats from R1 to R8 each contact one specific RNA base in the
eight-nucleotide RNA target. The functions of the N- and C-terminal
flanking repeats R1′ and R8′, however, remain unclear.
Here, we report how the repeats contribute to overall RNA binding.
We first prepared three mutants in which R1′ and/or… Show more
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