Necessity of detection of extended spectrum beta-lactamase, AmpC and metallo-beta-lactamases in Gram-negative bacteria isolated from clinical specimens

Haider, Mehvash; Rizvi, Meher; Fatima, Nazish; Shukla, Indu; Malik, Abida

doi:10.4103/0975-9727.128939

Cited by 14 publications

(12 citation statements)

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“…These results were less than outcomes of previous studies (Karn et al 2016) A (Dalela 2012) study showed that imipenem is the most active drug for the treatment of infections which are caused by ESBL producers, followed by Amikacin which is similar to our fi nding. 7.48% isolates were found to be MBL producers which is higher than Mishra et al 2012 (1.3%) and lesser than Haider et al 2014 (17.93%) highest MBL producer being Pseudomonas aeruginosa (Mishra et al 2012) and ( Haider et al 2014). And similar to previous study conducted at SGNHC which showed 8.4% were MBL producers (Chaudhary et al 2016).…”

Section: Methodssupporting

confidence: 70%

Prevalence of Extended Spectrum Beta Lactamases (ESBL) and Metallo Beta Lactamases (MBL) Mediated Resistance in Gram Negative Bacterial Pathogens

Pathak¹,

Jaishi

Yadav

et al. 2018

TU J. Microbiol.

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Objectives: This study was conducted to determine the prevalence of multi-drug resistance (MDR) along with Extended Spectrum β-lactamase (ESBL) and Metallo β-lactamase (MBL) producing gram negative bacterial isolates among the patients attending Shahid Gangalal National Heart Centre, Kathmandu, Nepal.Methods: This cross-sectional study was carried out from June to December; 2016. Altogether 977 clinical specimens were processed for analysis of bacteriological profile and the isolates were identified by culture, morphological and biochemical tests. Antibiotic susceptibility testing of the isolates was performed by Kirby Bauer disc diffusion methods following Clinical and Laboratories Standard Institute guideline and the isolates were tested for ESBL and MBL by combined disk method.Results: out of 977 clinical specimens, 254 (25.99%) were found to be gram negative bacterial isolates, among them Klebsiella pneumoniae 83 (32.67%) was the most predominant organism followed by E. coli 51 (20.07%), Pseudomonas aeruginosa 36 (14.17%), K. oxytoca 32 (12.59%), Proteus mirabilis 13 (5.11%) and P. vulgaris 13 (5.11%), Acinetobacter spp. 11 (4.33%), Citrobacter spp. 10 (3.93%) and Enterobacter spp. 5 (1.96%) respectively. 83 (32.67%) isolates were found to be MDR, 38(14.96%) were positive for ESBL while 19 (7.48%) were MBL producer.Conclusion: The determent drug resistance among ESBL and MBL producers, reflect the extensive use of antibiotics possessing difficulties in therapeutic potions in hospital setting which might be overcome by proper microbiological analysis of pathogenic isolates and judicious use of antibiotics for emergence of resistance strains.

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Section: Methodssupporting

confidence: 70%

Prevalence of Extended Spectrum Beta Lactamases (ESBL) and Metallo Beta Lactamases (MBL) Mediated Resistance in Gram Negative Bacterial Pathogens

Pathak¹,

Jaishi

Yadav

et al. 2018

TU J. Microbiol.

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“…In similar results, ceftazidime resistance was relatively higher among the isolates from inpatients which are 60.34, 65 and 73.4%, observed in studies done by different researchers (Easwaran et al, 2016;Umadevi et al, 2011;Haider et al, 2014). The resistance to ceftazidime is increasing at an alarming rate, complicating the clinical management of patients infected with P. aeruginosa isolates (Easwaran et al, 2016).…”

Section: Discussionmentioning

confidence: 53%

Antibiotic susceptibility pattern of Pseudomonas aeruginosa expressing blaGES and blaPER genes in two different hospitals

Ahmed¹,

Asghar²

2017

Afr. J. Biotechnol.

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Pseudomonas aeruginosa is intrinsically resistant to several antimicrobial drugs classes. Various extended-spectrum beta lactamases (ESBL) types have been found in P. aeruginosa such as Pseudomonas extended resistance (PER) and Guiana extended-spectrum (GES) enzymes. The study aimed to evaluate the susceptibility of the ESBL producing P. aeruginosa strains that express blaGES and blaPER genes to commonly used antibiotics. A total of 28 P. aeruginosa clinical isolates was identified as ESBL producers and subjected to polymerase chain reaction (PCR) technique for detection of blaGES, blaPER genes. Routine antimicrobial susceptibility was determined by the disc diffusion method. The highest resistance rate reached 71.4% for ciprofloxacin, while the lowest resistance rate (10.7%) was seen in imipenem followed by colistin (21.6%). blaGES gene was observed in 78.6% of the isolates, while blaPER appeared in 22.4%. It was concluded that imipenem and colistin showed good antipesuedomonal activity and blaGES was predominant gene among the ESBL producing P. aeruginosa in Makah hospitals. The results of the present study can help to prevent the mortality and morbidity associated with Pseudomonas infections in hospitals.

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“…In this study we observed, 4 (6.57%) AmpC producers, while AmpC production reported by Rawat et al (2013) 22 was 20.8% and 36.6% by Haider et al (2014). 23 All these were Inducible AmpC producers. However, Haider et al (2014) 23 have found only 21.7% Inducible AmpC producers and 78.3% non-inducible AmpC producers, which is an alarmingly high percentage of derepressed mutants.…”

Section: Discussionmentioning

confidence: 98%

Study of Organisms Causing Osteomyelitis in a Tertiary Care Hospital

Meshram¹,

Gajbhiye²,

Raut³

2020

jemds

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BACKGROUND The term osteomyelitis (OSM) was first used by the French surgeon Edouard Chassaignac in 1852, who defined the disease as an inflammatory process accompanied by bone destruction caused by an infecting microorganism. The clinical manifestation and the natural history of OSM depend on several factors. OSM mostly affects the growing ends of long bones. We wanted to study the organisms causing osteomyelitis and their antimicrobial susceptibility pattern. METHODS Pus and bone aspirate were collected from 115 diagnosed patients of osteomyelitis and were processed for isolation of organisms by standard microbiological techniques. Isolates were identified by various biochemical reactions and were subjected to antimicrobial susceptibility test as per CLSI guidelines by Kirby-Bauer disk diffusion technique on Mueller Hinton agar (MHA). Data collected in the questionnaire was entered and analysed in Epi Info software version 7.2. RESULTS In 101 samples, 116 organisms were isolated. In 14 samples no organism was isolated, which can be attributed to the viral aetiology, parasites and anaerobes. Acute Osteomyelitis (AOSM) was found to be more common in the age group of 1-10 years, whereas chronic osteomyelitis (COSM) was found more commonly in 21-30 and 31-40 years age group. Male to Female ratio was 2.2:1. Bones involved in AOSM and COSM were mostly femur followed by tibia and humerus. S. aureus was the most predominant isolate. All the isolates of S. aureus showed 100% sensitivity to Vancomycin, Amikacin, Netilmicin, Chloramphenicol. Out of 48 isolates of S. aureus, 37.50% were MRSA, 6.25% were ICR, 14.58% were MRSA+ICR found. CONCLUSIONS Osteomyelitis is found to be highest in third decade, with the males being predominantly affected. Acute osteomyelitis is predominantly seen in children, whereas chronic osteomyelitis in adults. Even though Staphylococcus aureus has always remained the most common etiological agent of osteomyelitis, increasing infections due to Gram negative bacilli and even poly-microbial infections are gaining importance. MRSA infection is known to increase post-operative complications. Introduction of MBL or carbapenemase production in Gram negative bacilli is a matter of great concern. Timely knowledge of aetiology and antimicrobial resistance pattern of osteomyelitis isolates can help in rational use of antibiotics and control of drug resistance.

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Necessity of detection of extended spectrum beta-lactamase, AmpC and metallo-beta-lactamases in Gram-negative bacteria isolated from clinical specimens

Cited by 14 publications

References 0 publications

Prevalence of Extended Spectrum Beta Lactamases (ESBL) and Metallo Beta Lactamases (MBL) Mediated Resistance in Gram Negative Bacterial Pathogens

Prevalence of Extended Spectrum Beta Lactamases (ESBL) and Metallo Beta Lactamases (MBL) Mediated Resistance in Gram Negative Bacterial Pathogens

Antibiotic susceptibility pattern of Pseudomonas aeruginosa expressing blaGES and blaPER genes in two different hospitals

Study of Organisms Causing Osteomyelitis in a Tertiary Care Hospital

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