Necessary and sufficient factors for the import of transfer RNA into the kinetoplast mitochondrion

Mukherjee, Saikat; Basu, Sandip K.; Home, Pratik; Dhar, Gunjan; Adhya, Samit

doi:10.1038/embor.2008.241

Cited by 3 publications

(2 citation statements)

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“…However, an editorial expression of concern regarding this study was published by the Proceedings of the National Academy of Sciences [88] . Two corrections to a subsequent paper describing Leishmania complex-mediated RNA import [89] have also been published 90 , 91 . It therefore remains unclear whether such machinery is effective in mammalian mitochondria.…”

Section: Import Of Exogenous Rna and Crispr/cas9 In Mammalian Mitochomentioning

confidence: 99%

Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized

2018

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In recent years mitochondrial DNA (mtDNA) has transitioned to greater prominence across diverse areas of biology and medicine. The recognition of mitochondria as a major biochemical hub, contributions of mitochondrial dysfunction to various diseases, and several high-profile attempts to prevent hereditary mtDNA disease through mitochondrial replacement therapy have roused interest in the organellar genome. Subsequently, attempts to manipulate mtDNA have been galvanized, although with few robust advances and much controversy. Re-engineered protein-only nucleases such as mtZFN and mitoTALEN function effectively in mammalian mitochondria, although efficient delivery of nucleic acids into the organelle remains elusive. Such an achievement, in concert with a mitochondria-adapted CRISPR/Cas9 platform, could prompt a revolution in mitochondrial genome engineering and biological understanding. However, the existence of an endogenous mechanism for nucleic acid import into mammalian mitochondria, a prerequisite for mitochondrial CRISPR/Cas9 gene editing, remains controversial.

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Section: Import Of Exogenous Rna and Crispr/cas9 In Mammalian Mitochomentioning

confidence: 99%

Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized

2018

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“…In this issue of EMBO reports , Mukherjee and colleagues provide a comprehensive description of the isolation and preliminary characterization of the RIC that mediates mitochondrial tRNA import in the kinetoplastid protozoon Leishmania tropica (Mukherjee et al , 2007). The authors affinity‐purified the RIC through a column containing a tRNA‐import signal oligonucleotide, and, by using mass spectrometry, the various components were identified.…”

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confidence: 99%