Near Infrared Spectroscopy Detection and Quantification of Herbal Medicines Adulterated with Sibutramine

Abstract

There is an increasing demand for herbal medicines in weight loss treatment. Some synthetic chemicals, such as sibutramine (SB), have been detected as adulterants in herbal formulations. In this study, two strategies using near infrared (NIR) spectroscopy have been developed to evaluate potential adulteration of herbal medicines with SB: a qualitative screening approach and a quantitative methodology based on multivariate calibration. Samples were composed by products commercialized as herbal medicines, as well as by laboratory adulterated samples. Spectra were obtained in the range of 14,000-4000 per cm. Using PLS-DA, a correct classification of 100% was achieved for the external validation set. In the quantitative approach, the root mean squares error of prediction (RMSEP), for both PLS and MLR models, was 0.2% w/w. The results prove the potential of NIR spectroscopy and multivariate calibration in quantifying sibutramine in adulterated herbal medicines samples.

Trends in digital detection for the quality and safety of herbs using infrared and Raman spectroscopy

“…NIRS coupled with PLS-DA distinguished PHPs adulterated with sibutramine with a correct classification of 100%. Four variables selected by MLR-SPA were executed to build a quantitative model ( Da Silva et al., 2015 ). Feng et al.…”

“…NIRS coupled with PLS-DA distinguished PHPs adulterated with sibutramine with a correct classification of 100%. Four variables selected by MLR-SPA were executed to build a quantitative model (Da Silva et al, 2015). Feng et al (2014) improved the reverse correlation coefficient (RCCM) for threshold settings to test antidiabetic PHM illegally added with synthetic drugs.…”

Detection and quantification of active pharmaceutical ingredients as adulterants in Garcinia cambogia slimming preparations using NIR spectroscopy combined with chemometrics

“…2A shows the derivative spectra of all adulterated samples at different levels of adulteration ranging from 0.5% to 10%, superimposed on that of Garcinia cambogia fruit peel powder derivative spectrum. The adulteration range was selected after consulting the literature for common adulteration ranges encountered in herbal medicines (Cheng et al, 2012;da Silva et al, 2015;Sarker, 2014). The best separation in HCA (Fig.…”

“…Thus, the development of a speedy screening, confirmation and quantitation method, with the purpose of identifying the synthetic weight-loss drugs in adulterated herbal slimming supplements, is inevitable to ensure human health and safety. Diverse studies applying various analytical techniques have been reported in the field of detection of synthetic anti-obesity drugs including TLC (Parodi et al, 1993), TLC-Micro IR (Csupor et al, 2013), HPLC (UPLC) (Bogusz et al, 2006), GC-MS (Haneef et al, 2013), NMR (Vaysse et al, 2010), immunochromatographic (Kanan et al, 2009) capillary zone electrophoresis (de Carvalho et al, 2010;Kanan et al, 2009) and infrared spectroscopic methods (da Silva et al, 2015;Feng et al, 2014).…”

“…When compared to other platforms of analysis, NIR spectroscopy reveals to have noticeable features especially its nondestructive nature of the analyzed sample and its capability of obtaining spectra for samples of different physical natures without or with only diminished pretreatment of such analyzed samples (Wang and Yu, 2015). There have been few previous reports for the application of NIR for the detection of synthetic adulterants in herbal slimming preparations (da Silva et al, 2015), yet these studies are limited to specific drugs such as sibutramine, or not displaying the ability of models in detection of low levels of adulteration by other possible APIs.…”