Near‐Infrared‐Light‐Triggered Antimicrobial Indium Phosphide Quantum Dots

Levy, Max; Bertram, John R.; Eller, Kristen A.; Chatterjee, Anushree; Nagpal, Prashant

doi:10.1002/ange.201906501

Cited by 10 publications

(16 citation statements)

References 27 publications

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“…There-fore, InP-based QDs emerged as an attractive alternative to CdSe QDs because they possess similar optoelectronic properties but a lower intrinsic toxicity, 3−5 which makes them suitable for applications spanning from optoelectronics to nanomedicine. 6,7 However, while the roles played by chemical composition, size and shape, 8 outer inorganic and organic shells 9 have been intensively investigated in toxicity studies, 10 other mechanisms need to be thoroughly dissected for a safe use of QDs in biomedicine. 11 These include the route of uptake and the intracellular trafficking events up to secretion.…”

Section: ■ Introductionmentioning

confidence: 99%

In Vivo Biotransformations of Indium Phosphide Quantum Dots Revealed by X-Ray Microspectroscopy

Veronesi

Moros

Castillo‐Michel

et al. 2019

ACS Appl. Mater. Interfaces

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Many attempts have been made to synthesize cadmium-free quantum dots (QDs), using nontoxic materials, while preserving their unique optical properties. Despite impressive advances, gaps in knowledge of their intracellular fate, persistence, and excretion from the targeted cell or organism still exist, precluding clinical applications. In this study, we used a simple model organism (Hydra vulgaris) presenting a tissue grade of organization to determine the biodistribution of indium phosphide (InP)-based QDs by X-ray fluorescence imaging. By complementing elemental imaging with In L-edge X-ray absorption near edge structure, unique information on in situ chemical speciation was obtained. Unexpectedly, spectral profiles indicated the appearance of In–O species within the first hour post-treatment, suggesting a fast degradation of the InP QD core in vivo, induced mainly by carboxylate groups. Moreover, no significant difference in the behavior of bare core QDs and QDs capped with an inorganic Zn(Se,S) gradient shell was observed. The results paralleled those achieved by treating animals with an equivalent dose of indium salts, confirming the preferred bonding type of In3+ ions in Hydra tissues. In conclusion, by focusing on the chemical identity of indium along a 48 h long journey of QDs in Hydra, we describe a fast degradation process, in the absence of evident toxicity. These data pave the way to new paradigms to be considered in the biocompatibility assessment of QD-based biomedical applications, with greater emphasis on the dynamics of in vivo biotransformations, and suggest strategies to drive the design of future applied materials for nanotechnology-based diagnosis and therapeutics.

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Section: ■ Introductionmentioning

confidence: 99%

In Vivo Biotransformations of Indium Phosphide Quantum Dots Revealed by X-Ray Microspectroscopy

Veronesi

Moros

Castillo‐Michel

et al. 2019

ACS Appl. Mater. Interfaces

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“…HeLa cells are commonly used as the model cell lines for toxicity assessment in vitro, and they were also adopted in this study. 52,53 After incubation with CWS NCs for 24 h, the viability of HeLa cells was approximately 100%, even at the concentration of CWS NCs up to 64 μg mL −1 (Figure 5a). SEM and elemental mapping images (Figure S19) show that few CWS NCs attach to the surface of HeLa cells, though HeLa cells were exposed to 100 μg mL −1 of CWS NCs.…”

Section: Resultsmentioning

confidence: 99%

Efficient Bacteria Killing by Cu₂WS₄ Nanocrystals with Enzyme-like Properties and Bacteria-Binding Ability

et al. 2019

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Antibacterial agents with high antibacterial efficiency and bacteria-binding capability are highly desirable. Herein, we describe the successful preparation of Cu2WS4 nanocrystals (CWS NCs) with excellent antibacterial activity. CWS NCs with small size (∼20 nm) achieve more than 5 log (>99.999%) inactivation efficiency of both Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) at low concentration (<2 μg mL–1) with or without ambient light, which is much better than most of the reported antibacterial nanomaterials (including Ag, TiO2, etc.) and even better than the widely used antibiotics (vancomycin and daptomycin). Antibacterial mechanism study showed that CWS NCs have both enzyme-like (oxidase and peroxidase) properties and selective bacteria-binding ability, which greatly facilitate the production of reactive oxygen species to kill bacteria. Animal experiments further indicated that CWS NCs can effectively treat wounds infected with methicillin-resistant Staphylococcus aureus (MRSA). This work demonstrates that CWS NCs have the potential as effective antibacterial nanozymes for the treatment of bacterial infection.

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“…22,23 It has been experimentally demonstrated that InP core/shell or InP core/alloy shell systems exhibit high ON times when probed in single-particle level. 24,25 Hydrophilic InP quantum dots (QDs) are used as targeted bioimaging agents in detection of early stage cancer, 26 as a therapeutic agent for the selective elimination of multidrug resistant bacteria in human cells, 27 and for immunoassays. 28 In a recent report, Dennis and co-workers have extended the emission characteristics of InP to the first optical tissue window (650−950 nm) using an inverted QD heterostructure (ZnSe/ InP/ZnS) and demonstrated its specific application for multiplexed fluorescence imaging.…”

Section: ■ Introductionmentioning

confidence: 99%

InP-Bovine Serum Albumin Conjugates as Energy Transfer Probes

et al. 2022

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The use of indium phosphide (InP) quantum dots (QDs) as biological fluorophores is limited by the low photoluminescence quantum yield (ϕPL) and the lack of effective bioconjugation strategies. The former issue has been addressed by introducing a strain relaxing intermediate shell such as ZnSe, GaP etc. that significantly enhances the ϕPL of InP. Herein, we present an effective strategy for the conjugation of emissive InP/GaP/ZnS QDs with a commonly used globular protein, namely bovine serum albumin (BSA), which generate colloidally stable QD bioconjugates, labeled as InP-BSA and demonstrate its use as energy transfer probes. The conjugate contains one protein per QD, and the circular dichroism spectra of BSA and InP-BSA exhibit similar fractions of α-helix and β-sheet, reflective of the fact that the secondary structure of the protein is intact on binding. More importantly, the fluorescence polarization studies corroborate the fact that the bound protein can hold a variety of chromophoric acceptors. Upon selectively exciting the InP-BSA component in the presence of bound chromophores, a reduction in the emission intensity of the donor is observed with a concomitant increase in emission of the acceptor. Time-resolved investigations further confirm an efficient nonradiative energy transfer from InP-BSA to the bound acceptors.

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Near‐Infrared‐Light‐Triggered Antimicrobial Indium Phosphide Quantum Dots

Cited by 10 publications

References 27 publications

In Vivo Biotransformations of Indium Phosphide Quantum Dots Revealed by X-Ray Microspectroscopy

In Vivo Biotransformations of Indium Phosphide Quantum Dots Revealed by X-Ray Microspectroscopy

Efficient Bacteria Killing by Cu₂WS₄ Nanocrystals with Enzyme-like Properties and Bacteria-Binding Ability

InP-Bovine Serum Albumin Conjugates as Energy Transfer Probes

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Near‐Infrared‐Light‐Triggered Antimicrobial Indium Phosphide Quantum Dots

Cited by 10 publications

References 27 publications

In Vivo Biotransformations of Indium Phosphide Quantum Dots Revealed by X-Ray Microspectroscopy

In Vivo Biotransformations of Indium Phosphide Quantum Dots Revealed by X-Ray Microspectroscopy

Efficient Bacteria Killing by Cu2WS4 Nanocrystals with Enzyme-like Properties and Bacteria-Binding Ability

InP-Bovine Serum Albumin Conjugates as Energy Transfer Probes

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Product

Resources

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Efficient Bacteria Killing by Cu₂WS₄ Nanocrystals with Enzyme-like Properties and Bacteria-Binding Ability