Near-infrared LEDS provide persistent and increasing protection against E. faecalis

Petrini, Morena; Spoto, Giuseppe; Scarano, Antônio; D’Arcangelo, Camillo; Tripodi, Domenico; Fermo, Paola Di; D’Ercole, Simonetta

doi:10.1016/j.jphotobiol.2019.111527

Cited by 20 publications

(16 citation statements)

References 24 publications

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“…In our recent studies, we focused on photoinactivation using light-emitting diodes (LEDs) against common pathogens of the oral cavity. In particular, near-infrared light (NIR) showed an antibacterial effect against Enterococcus faecalis and Pseudomonas aeruginosa [12][13][14][15]. This was confirmed over time and was effective against both planktonic and sessile bacteria [12][13][14][15].…”

Section: Introductionmentioning

confidence: 97%

Photodynamic Antibiofilm and Antibacterial Activity of a New Gel with 5-Aminolevulinic Acid on Infected Titanium Surfaces

et al. 2022

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The use of a new gel containing aminolevulinic acid and red light (ALAD–PDI) was tested in order to counteract bacterial biofilm growth on different titanium implant surfaces. The varying antibacterial efficacy of ALAD–PDI against biofilm growth on several titanium surfaces was also evaluated. A total of 60 titanium discs (30 machined and 30 double-acid etched, DAE) were pre-incubated with saliva and then incubated for 24 h with Streptococcus oralis to form bacterial biofilm. Four different groups were distinguished: two exposed groups (MACHINED and DAE discs), covered with S. oralis biofilm and subjected to ALAD + PDI, and two unexposed groups, with the same surfaces and bacteria, but without the ALAD + PDI (positive controls). Negative controls were non-inoculated discs alone and combined with the gel (ALAD) without the broth cultures. After a further 24 h of anaerobic incubation, all groups were evaluated for colony-forming units (CFUs) and biofilm biomass, imaged via scanning electron microscope, and tested for cell viability via LIVE/DEAD analysis. CFUs and biofilm biomass had significantly higher presence on unexposed samples. ALAD–PDI significantly decreased the number of bacterial CFUs on both exposed surfaces, but without any statistically significant differences among them. Live/dead staining showed the presence of 100% red dead cells on both exposed samples, unlike in unexposed groups. Treatment with ALAD + red light is an effective protocol to counteract the S. oralis biofilm deposited on titanium surfaces with different tomography.

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Section: Introductionmentioning

confidence: 97%

Photodynamic Antibiofilm and Antibacterial Activity of a New Gel with 5-Aminolevulinic Acid on Infected Titanium Surfaces

et al. 2022

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“…The E. faecalis cell viability was evaluated by a fluorescent microscopy using a BacLight LIVE/DEAD Viability Kit (Molecular Probes, Invitrogen detection technologies, USA) as previously described [ 5 , 6 ]. After treatments, 1 mL of the bacterial suspension was concentrated by centrifugation at 10,000× g for 10–15 min, washed with PBS, and stained as indicated by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, the increase of the problem of antibiotic resistance promoted the dissemination of resistant bacteria and the spreading of iatrogenic infections [ 3 ]. In the recent years, the use of light-devices, such as laser and light emitting diodes (LEDs) showed encouraging results against different types of bacteria and yeasts [ 2 , 4 , 5 , 6 , 7 ]. The photoinactivation can be induced by the light activation of endogenous photosensitizers present inside the bacterial cells or by exogenous substances applied by the clinicians, such as in photodynamic therapy (PDT).…”

Section: Introductionmentioning

confidence: 99%

Comparison between Single and Multi-LED Emitters for Photodynamic Therapy: An In Vitro Study on Enterococcus faecalis and Human Gingival Fibroblasts

Petrini

Pierfelice

D’Amico

et al. 2022

IJERPH

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Aim of the study: The aim was to evaluate the effects of two LED devices, TL-01 and TL-03 in photodynamic therapy (PDT), on Enterococcus faecalis and on human gingival fibroblasts (HGFs). TL-01, characterized by a single emitter, irradiates one periodontal site at a time, whereas the multi-led device (TL-03) irradiates all vestibular sites of a single arch at a time. Methods: E. faecalis bacterial suspensions and HGFs were incubated for 45 min with Aladent gel (ALAD) containing 5-aminolevulinic acid and then exposed to LED devices (ALAD-PDT), having different distance and timing of irradiation (TL-01 N (0.5 mm, for 7 min), TL-03 N (0.5 mm, 15 min) and TL-03 F (30.0 mm, 15 min)). For bacterial suspension, the colony forming units and the live/dead staining were evaluated after 24 h, while the protoporphyrin IX (PpIX) content was monitored in all phases of the experimentation. For HGFs, the cell viability, proliferation, cell morphology, and adhesion were evaluated at 24 h. Results: Both TL-01 and TL-03 showed a significant reduction of bacterial load. The photoinactivation was inversely proportional to the PpIX accumulation. TL-01 and TL-03 promoted proliferation and adhesion of HGFs. Conclusions: Both tested devices for ALAD-PDT were equally effective in significantly reducing Enterococcus faecalis growth and in promoting HGFs proliferation and adhesion, in vitro.

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“…In previous studies, we have shown that light emitting diodes (LEDs) at 880 nm could provide an antibacterial effect on Enterococcus faecalis , a very resistant pathogen that is usually the cause of primary and secondary endodontic failures, both in the planktonic and in the biofilm aggregation form [ 6 , 7 , 8 ]. In particular, this antibacterial action seemed to increase over time [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Complex Electromagnetic Fields Reduce Candida albicans Planktonic Growth and Its Adhesion to Titanium Surfaces

et al. 2021

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This study evaluates the effects of different programs of complex electromagnetic fields (C.M.F.s) on Candida albicans, in planktonic and sessile phase and on human gingival fibroblasts (HGF cells). In vitro cultures of C. albicans ATCC 10231 and HGF cells were exposed to different cycles of C.M.F.s defined as: oxidative stress, oxidative stress/antibacterial, antibacterial, antibacterial/oxidative stress. Colony forming units (CFUs), metabolic activity, cells viability (live/dead), cell morphology, filamentation analysis, and cytotoxicity assay were performed. The broth cultures, exposed to the different C.M.F.s, were grown on titanium discs for 48 h. The quantity comparisons of adhered C. albicans on surfaces were determined by CFUs and scanning electron microscopy. The C. albicans growth could be readily controlled with C.M.F.s reducing the number of cultivable planktonic cells vs. controls, independently by the treatment applied. In particular, the antibacterial program was associated with lower levels of CFUs. The quantification of the metabolic activity was significantly lower by using the oxidative stress program. Live/dead images showed that C.M.F.s significantly decreased the viability of C. albicans. C.M.F.s inhibited C. albicans virulence traits reducing hyphal morphogenesis, adhesion, and biofilm formation on titanium discs. The MTS assay showed no negative effects on the viability of HGF. Independent of the adopted protocol, C.M.F.s exert antifungal and anti-virulence action against C. albicans, no cytotoxicity effects on HGF and can be useful in the prevention and treatment of yeast biofilm infections.

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Near-infrared LEDS provide persistent and increasing protection against E. faecalis

Cited by 20 publications

References 24 publications

Photodynamic Antibiofilm and Antibacterial Activity of a New Gel with 5-Aminolevulinic Acid on Infected Titanium Surfaces

Photodynamic Antibiofilm and Antibacterial Activity of a New Gel with 5-Aminolevulinic Acid on Infected Titanium Surfaces

Comparison between Single and Multi-LED Emitters for Photodynamic Therapy: An In Vitro Study on Enterococcus faecalis and Human Gingival Fibroblasts

Complex Electromagnetic Fields Reduce Candida albicans Planktonic Growth and Its Adhesion to Titanium Surfaces

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