Near-Infrared Illumination of Native Tissues for Image-Guided Surgery

Owens, Eric A.; Hyun, Hoon; Dost, Tyler L.; Lee, Jeong Heon; Park, GwangLi; Pham, Dang Huan; Park, Min Ho; Choi, Hak; Henary, Maged

doi:10.1021/acs.jmedchem.6b00038

Cited by 51 publications

(47 citation statements)

References 28 publications

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“…NIR light is featured with reduced scattering, minimal tissue absorption with high tissue penetration depth, and low tissue autofluorescence interference compared with visible light, and therefore suitable for bioimaging with high signal-to-background ratio and resolution of deep tissues. [1][2][3][4][5] In addition, it shows minimum genotoxicity. 6 These characteristics collectively favor the therapeutic use of NIR light in the clinic.…”

Section: Introductionmentioning

confidence: 99%

High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells

et al. 2020

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Significance: Photobiomodulation is a well-established therapeutic modality. However, the mechanism of action is poorly understood, due to lack of research in the causal relationship between the near-infrared (NIR) light irradiation and its specific biological effects, hindering broader applications of this technology. Aim: Since biological chromophores typically show several absorption peaks, we determined whether specific effects of photobiomodulation are induced with a combination of two wavelengths at a certain range of irradiance only, rather than a single wavelength of NIR light. Approach: In order to analyze a wide array of combinations of multispectral NIR light at various irradiances efficiently, we developed a new optical platform equipped with two distinct wavelengths of NIR lasers by high-throughput multiple dosing for single-cell live imaging. Two wavelengths of 1064 and 1270 nm were selected based on their photobiomodulatory effects reported in the literature. Results: A specific combination of wavelengths at low irradiances (250 to 400 mW∕cm 2 for 1064 nm and 55 to 65 mW∕cm 2 for 1270 nm) modulates mitochondrial retrograde signaling, including intracellular calcium and reactive oxygen species in T cells. The time-dependent density functional theory computation of binding of nitric oxide (NO) to cytochrome c oxidase indicates that the illumination with NIR light could result in the NO release, which might be involved in these changes. Conclusions: This optical platform is a powerful tool to study causal relationship between a specific parameter of NIR light and its biological effects. Such a platform is useful for a further mechanistic study on not only photobiomodulation but also other modalities in photomedicine.

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Section: Introductionmentioning

confidence: 99%

High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells

et al. 2020

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“…A major focus of BNT diagnostics is the development of cancer imaging techniques to identify and locate cancer cells. For example, an in vivo tumor imaging technology is being developed, called in vivo fluorescent guided therapy, which fluorescently illuminates every cancerous cell in the patient in order to ensure complete and precise surgical removal (69). This technique even illuminates cancer cells that have not yet formed into visible tumors, which reduces the risk of cancer recurrence after surgery (69).…”

Section: Surgical Application Of Theranostic Nanoparticlesmentioning

confidence: 99%

“…The combination of diagnostic techniques and bioinformatics allows the most unique, specific biomarkers for each type of cancer to be identified for use as the target molecule, which ensures that only cancerous cells will be targeted. This technique has been successful in mouse models and is on its way to becoming a common technique for the enhancement of precision surgery (69)(70)(71).…”

Section: Surgical Application Of Theranostic Nanoparticlesmentioning

confidence: 99%

Nanobiotechnology medical applications: Overcoming challenges through innovation

Singer

Markoutsa

Limayem

et al. 2018

The EuroBiotech Journal

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Biomedical Nanotechnology (BNT) has rapidly become a revolutionary force that is driving innovation in the medical field. BNT is a subclass of nanotechnology (NT), and often operates in cohort with other subclasses, such as mechanical or electrical NT for the development of diagnostic assays, therapeutic implants, nano-scale imaging systems, and medical machinery. BNT is generating solutions to many conventional challenges through the development of enhanced therapeutic delivery systems, diagnostic techniques, and theranostic therapies. Therapeutically, BNT has generated many novel nanocarriers (NCs) that each express specifically designed physiochemical properties that optimize their desired pharmacokinetic profile. NCs are also being integrated into nanoscale platforms that further enhance their delivery by controlling and prolonging their release profile. Nano-platforms are also proving to be highly efficient in tissue regeneration when combined with the appropriate growth factors. Regarding diagnostics, NCs are being designed to perform targeted delivery of luminescent tags and contrast agents that enhance the NC -aided imaging capabilities and resulting diagnostic accuracy of the presence of diseased cells. This technology has also been advancing the ability for surgeons to practice true precision surgical techniques. Incorporating therapeutic and diagnostic NC-components within a single NC can facilitate both functions, referred to as theranostics, which facilitates real-time in vivo tracking and observation of drug release events via enhanced imaging. Additionally, stimuli-responsive theranostic NCs are quickly developing as vectors for tumor ablation therapies by providing a model that facilitates the location of cancer cells for the application of an external stimulus. Overall, BNT is an interdisciplinary approach towards health care, and has the potential to significantly improve the quality of life for humanity by significantly decreasing the treatment burden for patients, and by providing non-invasive therapeutics that confer enhanced therapeutic efficiency and safety

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“…The primary goal of cancer surgery is to maximize the tumor excision and minimize the collateral damage. Molecular imaging techniques are required to achieve these goals, leading to "image-guided surgery" [108][109][110][111][112][113][114][115][116][117]. Especially, optical imaging is the most suitable for imageguided surgery (or targeted surgery) because fluorescence signals can provide real-time guidance to differentiate positive tumor margins and local malignant masses from normal tissues during surgery, thereby increasing the long-term patient survival.…”

Section: Optical Imaging Guided Surgerymentioning

confidence: 99%

Recent Advances in Bioimaging for Cancer Research

Lim¹,

Son²,

Lim³

2018

State of the Art in Nano-Bioimaging

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Molecular imaging techniques as well as nanoparticle applicable to molecular imaging are being explored to improve the cancer detection accuracy, which help to manage efficiently at the early stage. Among the various imaging technologies, optical imaging is a highly sensitive detection technique that allows direct observation of specific molecular events, biological pathways, and disease processes in real time through imaging probes that emit light in a range of wavelengths. Recently, nanoparticles have provided significant progresses that can be simultaneously used for cancer diagnosis and therapy (cancer theranostics). Theranostics aims to provide "image-guided cancer therapy," by integrating therapeutic and imaging agents in a single platform. In addition, molecular imaging techniques facilitate "image-guided surgery" enabling maximization of tumor excision and minimization of side effects. The optical signals generated by fluorescence nanoparticles offer the possibility to distinguish tumor sites and normal tissues during surgery by real-time guidance, thereby increasing the long-term patient survival. These techniques will considerably contribute to reducing cancer recurrence and developing more effective cures. In this chapter, we will introduce diverse research on nanomaterials-based optical imaging for effective cancer therapy.

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Near-Infrared Illumination of Native Tissues for Image-Guided Surgery

Cited by 51 publications

References 28 publications

High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells

High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells

Nanobiotechnology medical applications: Overcoming challenges through innovation

Recent Advances in Bioimaging for Cancer Research

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