Near‐infrared Fourier transform Raman spectroscopic study of human brain tissues and tumours

Abstract

Non-destructive examination of human brain tissues and several kinds of brain tumours using near-infrared (1064 om) excited Fourier transform (NIR-FT) Raman spectroscopy is reported. The spectra from normal but oedematous grey and white matter were similar to those of normal rat grey and white matter reported previously. The brain tumours investigated (glioma grade I1 and 111, two acoustic neurinomas and central neurocytoma) gave Raman spectra essentially the same as to that of grey matter. Histological examination of glioma grade I11 revealed extracellular PAS-positive materials, which have a band at 856 cm-' due to polysaccharides. One of the neurinoma contained a carotenoid which exhibited two resonance Raman peaks at 1157 and 1524 cm-'; another neurinoma did not contain the carotenoid. The appearance of a strong Raman peak at 960 cm-' suggested that the central neurocytoma of choroid plexus was calcified.

Improved protocols for vibrational spectroscopic analysis of body fluids

“…agnostic applications has been well investigated and demonstrated, notably in dermal applications [3][4][5][6] but also for many other anatomical sites, including cervix [7,8], skin [9][10][11], lung [12,13], brain [14], oesophagus [15,16], colon [17], prostate [18], nasopharynx [19], larynx [20], oral [21], breast [22,23] and liver [24]. The detailed information of the molecular structure and composition of the tissue provided by Raman and Fourier Transform Infrared absorption (FTIR) spectroscopy ultimately promises an analysis of disease origin and progression.…”

A comparison of Raman, FTIR and ATR-FTIR micro spectroscopy for imaging human skin tissue sections

“…In the past, many studies have been carried out using either Raman or Fourier Transform Infrared absorption (FTIR) spectroscopy to classify tissue with a view to disease diagnosis. Some of the tissue types examined by various groups include cervical 2, 3 skin 47 ,lung 8,9 , brain 10 , oesophagus 11,12 , colon 13 , prostate 14 , nasopharynx 15 , larynx 16 oral 17 , breast 18,19 , liver 20 . However, although often referred to as complementary techniques, Raman and FTIR spectroscopy are based on very different fundamental physical phenomena, and thus present different relative advantages and disadvantages, and indeed technical challenges to their clinical implementation.…”

Classification of colonic tissues using near-infrared Raman spectroscopy and support vector machines

“…In the past decade, optical spectroscopic methods such as Raman spectroscopy have been comprehensively investigated for cancer and precancer diagnosis and evaluation (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). Raman spectroscopy measures inelastic light scattering and is a vibrational spectroscopic technique that can provide specific spectroscopic fingerprints based on the molecular composition and structures of biological tissues (3,4,(8)(9)(10).…”

“…Less water absorption makes it easy to detect other tissue components and results in deeper light penetration into the tissue. As such, NIR Raman spectroscopy has received great interest for in vivo and in vitro diagnosis of malignancies in a number of organs including the colon (3,(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). NIR Raman spectroscopy studies showed that tissue Raman spectral features could be used to correlate with the molecular and structural changes associated with neoplastic transformations (5,7,10,12), demonstrating the feasibility of NIR Raman spectroscopy technique for early cancer detection.…”