Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes in Neuroimaging

Piatkevich, Kiryl D.; Suk, Ho Jun; Kodandaramaiah, Suhasa B.; Yoshida, Fumiaki; DeGennaro, Ellen M; Drobizhev, Mikhail; Hughes, Thomas E.; Desimone, Robert; Boyden, Edward S.; Verkhusha, Vladislav V.

doi:10.1016/j.bpj.2017.09.007

Cited by 49 publications

(56 citation statements)

References 45 publications

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“…Shifting fluorescence of GECIs into the near-infrared range will provide completely crosstalk-free coupling with optogenetic tools. Near-infrared fluorescent proteins derived from phytochomes [63] have shown to be useful for labeling neurons in vivo in mammals [64], zebrafish [65], and flies [66]. Near-infrared light is also beneficial for the noninvasive whole-body imaging of small mammals [67,68] due to reduced autofluorescence, low light scattering, and minimal absorbance at longer wavelengths.…”

Section: Introductionmentioning

confidence: 99%

Advances in Engineering and Application of Optogenetic Indicators for Neuroscience

2019

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Our ability to investigate the brain is limited by available technologies that can record biological processes in vivo with suitable spatiotemporal resolution. Advances in optogenetics now enable optical recording and perturbation of central physiological processes within the intact brains of model organisms. By monitoring key signaling molecules noninvasively, we can better appreciate how information is processed and integrated within intact circuits. In this review, we describe recent efforts engineering genetically-encoded fluorescence indicators to monitor neuronal activity. We summarize recent advances of sensors for calcium, potassium, voltage, and select neurotransmitters, focusing on their molecular design, properties, and current limitations. We also highlight impressive applications of these sensors in neuroscience research. We adopt the view that advances in sensor engineering will yield enduring insights on systems neuroscience. Neuroscientists are eager to adopt suitable tools for imaging neural activity in vivo, making this a golden age for engineering optogenetic indicators.

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Section: Introductionmentioning

confidence: 99%

Advances in Engineering and Application of Optogenetic Indicators for Neuroscience

2019

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“…[ 32,33 ] Recently, a series of NIR‐emitting fluorescent proteins with an emission region of 650–800 nm have been developed and successfully applied in bioimaging with high signal‐to‐background ratio and sensitivity. [ 34–37 ] For example, the first NIR‐emitting fluorescent protein was developed by Shu and colleagues using a bacterial phytochrome scaffold (DrBphP). The bright fluorescent protein, IFP1.4, can emit in the NIR‐I window with a peak emission of 708 nm.…”

Section: Near‐infrared Light and Its Biomedical Applicationsmentioning

confidence: 99%

Advanced Near‐Infrared Light for Monitoring and Modulating the Spatiotemporal Dynamics of Cell Functions in Living Systems

et al. 2020

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Light‐based technique, including optical imaging and photoregulation, has become one of the most important tools for both fundamental research and clinical practice, such as cell signal sensing, cancer diagnosis, tissue engineering, drug delivery, visual regulation, neuromodulation, and disease treatment. In particular, low energy near‐infrared (NIR, 700–1700 nm) light possesses lower phototoxicity and higher tissue penetration depth in living systems as compared with ultraviolet/visible light, making it a promising tool for in vivo applications. Currently, the NIR light‐based imaging and photoregulation strategies have offered a possibility to real‐time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy. Herein, the recent progress with respect to NIR light for monitoring and modulating the spatiotemporal dynamics of cell functions in living systems are summarized. In particular, the applications of NIR light‐based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed. In addition, the challenges and prospects for NIR light‐based cell sensing and regulating techniques are comprehensively discussed.

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“…Although, due to the low level of BV in neurons, their visualization using biomark ers with a moderate affinity to BV, such as NIR FPs from the IFP, mIFP, and smURFP series, requires introduc tion of exogenous BV or co expression of HO [17,20,24,29]. However, the use of NIR FPs from the iRFP and miRFP series with a relatively high affinity to BV elimi nated the need for exogenous BV in the labeling of pri mary hippocampal neurons [29,87], retinal neurons [88], and mouse and rat cortical neurons in vivo [75,81,87]. One must take into account that in an organism, BV par ticipates in the cell defense against oxidative stress [89] and activation of signaling pathways [90 92], as well as affects cell viability and proliferation [93].…”

Section: Application Of Nir Fluorescent Biomarkers Reporters and Bimentioning

confidence: 99%

Near-Infrared Fluorescent Proteins and Their Applications

Karasev

Stepanenko

Rumyantsev

et al. 2019

Biochemistry Moscow

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High transparency, low light scattering, and low autofluorescence of mammalian tissues in the near infrared (NIR) spectral range (~650 900 nm) open a possibility for in vivo imaging of biological processes at the micro and macroscales to address basic and applied problems in biology and biomedicine. Recently, probes that absorb and fluoresce in the NIR optical range have been engineered using bacterial phytochromes-natural NIR light absorbing photoreceptors that regulate metabolism in bacteria. Since the chromophore in all these proteins is biliverdin, a natural product of heme catabolism in mammalian cells, they can be used as genetically encoded fluorescent probes, similarly to GFP like fluores cent proteins. In this review, we discuss photophysical and biochemical properties of NIR fluorescent proteins, reporters, and biosensors and analyze their characteristics required for expression of these molecules in mammalian cells. Structural features and molecular engineering of NIR fluorescent probes are discussed. Applications of NIR fluorescent proteins and biosensors for studies of molecular processes in cells, as well as for tissue and organ visualization in whole body imaging in vivo, are described. We specifically focus on the use of NIR fluorescent probes in advanced imaging technologies that com bine fluorescence and bioluminescence methods with photoacoustic tomography.

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Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes in Neuroimaging

Cited by 49 publications

References 45 publications

Advances in Engineering and Application of Optogenetic Indicators for Neuroscience

Advances in Engineering and Application of Optogenetic Indicators for Neuroscience

Advanced Near‐Infrared Light for Monitoring and Modulating the Spatiotemporal Dynamics of Cell Functions in Living Systems

Near-Infrared Fluorescent Proteins and Their Applications

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