Near infrared fluorescent probes for detecting and imaging active small molecules

Ding, Caiping; Ren, Tian‐Bing

doi:10.1016/j.ccr.2023.215080

Cited by 67 publications

(23 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Small-molecule fluorescent probes have become powerful tools in sensing and imaging due to their high sensitivity, good specificity, fast response, and technical simplicity. − In fluorescence sensing, the detection of trace analytes is generally achieved through one or more optical physical mechanisms. Among these, the photoinduced electron transfer (PET) effect has attracted extensive attention. − The PET effect is characterized by the intramolecular electron transfer from the electron donor to the nearby fluorophore (acceptor), which occurs when the donor’s highest occupied molecular orbital (HOMO) energy level lies between the lowest unoccupied molecular orbital (LUMO) and HOMO of the fluorophore.…”

mentioning

confidence: 99%

Photoinduced Electron Transfer Modulated Photoelectric Signal: Toward an Organic Small Molecule-Based Photoelectrochemical Platform for Formaldehyde Detection

Gao

Huang

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

Photoelectrochemical (PEC) sensing has been rapidly evolving in recent years, while the introduction of small molecules with specific recognition functions into the sensing interface remains a nascent area of study. In this work, we reported a PEC biosensor for formaldehyde (FA) detection based on photoinduced electron transfer (PET)-gated electron injection between organic small molecules and inorganic semiconducting substrates. Specifically, an FA-responsive probe (NA-FA-COOH) and TiO2 nanoarrays were integrated to construct a PEC platform (NFC/TiO2) via a coordination bond. NFC served simultaneously as a target-specific recognition element and a modulator of photoinduced electron injection. Treatment of NFC/TiO2 by FA would suppress the intramolecular PET process, with the quenched photocurrent signal due to the changed carrier transfer pathway, thus establishing the PEC platform for FA based on effective PET modulation. The proposed PEC system exhibited high selectivity and sensitivity, with a low detection limit of 0.071 μM. This study presents a novel perspective on the use of organic small molecules with a PET effect for advanced PEC bioanalysis.

show abstract

mentioning

confidence: 99%

Photoinduced Electron Transfer Modulated Photoelectric Signal: Toward an Organic Small Molecule-Based Photoelectrochemical Platform for Formaldehyde Detection

Gao

Huang

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…During the design of luminescent molecular probes, organic fluorophores, and metal complexes are commonly used fluorophores. Organic fluorophores, characterized by their high fluorescence quantum yields and easily adjustable absorption and emission wavelength ranges, have emerged as indispensable tools for diverse bioimaging applications. , Transition metal complexes, including iridium(III) complexes, exhibit attractive features for the development of luminescent probes, encompassing their long luminescence lifetimes, excellent optical and chemical stabilities, and large Stokes shifts. − Moreover, iridium(III) complexes have their own two-photon excitation capability as well as the ability to modulate emission wavelength and intensity through ligand structure variations, which makes them widely utilized in investigating physiologically active species. − However, there are few studies on the combination of organic fluorophores and metal complexes to develop ratio-type luminescent probes that can improve the accuracy of the specific detection of physiologically active species. In our previous works, some ratiometric luminescent probes combining the merits of ruthenium(II) complexes and organic fluorophores had been constructed for detecting reactive oxygen, nitrogen, and carbonyl species in biological systems. − …”

Section: Introductionmentioning

confidence: 99%

FRET Luminescent Probe for the Ratiometric Imaging of Peroxynitrite in Rat Brain Models of Epilepsy-Based on Organic Dye-Conjugated Iridium(III) Complex

Gao,

Zhang,

Dong

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

“…Optical imaging has the advantages of noninvasiveness, high sensitivity, and nonradiation. − Near-infrared (NIR) optical probes, in particular, enhance tissue penetration depth and exhibit significant potential in biomedical applications. − Activatable probes can respond to the biomarkers of interest, causing the probe signal to change from the initial “off” state to the “on” state with a high signal-to-noise ratio (SNR). − Among them, activatable organic optical probes have garnered increasing attention due to their facile synthesis, cost-effectiveness, good biocompatibility, and capacity for real-time, noninvasive imaging of biomarkers with high contrast and sensitivity. − However, there are still few NIR activatable organic optical probes for evaluating the efficacy of radiotherapy …”

Section: Introductionmentioning

confidence: 99%

A Self-Assembled Organic Probe with Activatable Near-Infrared Fluoro-Photoacoustic Signals for In Vivo Evaluation of the Radiotherapy Effect

An,

Gu,

Miao

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

Early evaluation and prediction of the radiotherapy effect against tumors are crucial for effective radiotherapy management. The clinical approach generally relies on anatomical changes in tumor size, which is unable to promptly reflect clinical outcomes and guide a timely adjustment of therapy regimens. To resolve it, we herein develop a self-assembled organic probe (dCyFFs) with caspase-3 (Casp-3)-activatable near-infrared (NIR) fluoro-photoacoustic signals for early evaluation and prediction of radiotherapy efficacy. The probe contains an NIR dye that is caged with a Casp-3-cleavable substrate and linked to a self-assembly initiating moiety. In the presence of Casp-3, the self-assembled probe can undergo secondary assembly into larger nanoparticles and simultaneously activate NIR fluoro-photoacoustic signals. Such a design endows a superior real-time longitudinal imaging capability of Casp-3 generated by radiotherapy as it facilitates the passive accumulation of the probe into tumors, activated signal output with enhanced optical stability, and retention capacity relative to a nonassembling small molecular control probe (dCy). As a result, the probe enables precise prediction of the radiotherapy effect as early as 3 h posttherapy, which is further evidenced by the changes in tumor size after radiotherapy. Overall, the probe with Casp-3-mediated secondary assembly along with activatable NIR fluoro-photoacoustic signals holds great potential for evaluating and predicting the response of radiotherapy in a timely manner, which can also be explored for utilization in other therapeutic modalities.

show abstract

Near infrared fluorescent probes for detecting and imaging active small molecules

Cited by 67 publications

References 131 publications

Photoinduced Electron Transfer Modulated Photoelectric Signal: Toward an Organic Small Molecule-Based Photoelectrochemical Platform for Formaldehyde Detection

Photoinduced Electron Transfer Modulated Photoelectric Signal: Toward an Organic Small Molecule-Based Photoelectrochemical Platform for Formaldehyde Detection

FRET Luminescent Probe for the Ratiometric Imaging of Peroxynitrite in Rat Brain Models of Epilepsy-Based on Organic Dye-Conjugated Iridium(III) Complex

A Self-Assembled Organic Probe with Activatable Near-Infrared Fluoro-Photoacoustic Signals for In Vivo Evaluation of the Radiotherapy Effect

Contact Info

Product

Resources

About