Near-infrared fluorescent probe for the simultaneous dual-color monitoring of pH changes in two organelles

A dual-labeling fluorescent probe to track lysosomal polarity and endoplasmic reticulum dynamics during ferroptosis

“…20 To date, only four fluorescent probes for ER/lysosome dual-labeling have been successfully developed (Table S3 and Scheme S3, ESI†). 21–24 Nevertheless, these pH-sensitive lipophilic probes cannot detect polarity and tend to transfer into the newborn LDs in ferroptosis. To the best of our knowledge, no probe has been reported that simultaneously detects polarity and discriminates between the ER and lysosomes (Scheme 1).…”