Near-Full-Length Genome Sequences Representing an Event of Zooanthroponotic Transmission of SARS-CoV-2 Lineage B.1.189 in Mexico during 2020

Navarro-Lopez, Roberto; Solís-Hernández, Mario; Rocha-Martinez, Marisol K; Eberl, Samantha; Gómez‐Romero, Ninnet; Velazquez‐Salinas, Lauro; Estrada-Franco, José G.

doi:10.1128/mra.00497-22

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…However, this is not the first report of dog infection in Mexico because SENASICA previously reported another case to the OMSA [ 33 ]. Interestingly, other dogs in contact with infected persons were negative for RT–qPCR but positive for antibodies [ 14 , 38 , 39 ]. One explanation is that SARS-CoV-2 has a low replication rate in dogs or that the infection occurred weeks before sample collection.…”

Section: Discussionmentioning

confidence: 99%

Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico

Dehesa-Canseco,

Pastrana-Unzueta,

Carrillo-Guzmán

et al. 2023

Pathogens

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SARS-CoV-2 mainly affects humans; however, it is important to monitor the infection of companion and wild animals as possible reservoirs of this virus. In this sense, seroprevalence studies in companion animals, such as dogs and cats, provide important information about the epidemiology of SARS-CoV-2. This study aimed to evaluate the seroprevalence of neutralizing antibodies (nAbs) against the ancestral strain and the Omicron BA.1 subvariant in dogs and cats in Mexico. Six hundred and two samples were obtained from dogs (n = 574) and cats (n = 28). These samples were collected from the end of 2020 to December 2021 from different regions of Mexico. The presence of nAbs was evaluated using a plaque reduction neutralization test (PRNT) and microneutralization (MN) assays. The results showed that 14.2% of cats and 1.5% of dogs presented nAbs against the ancestral strain of SARS-CoV-2. The analysis of nAbs against Omicron BA.1 in cats showed the same percentage of positive animals but a reduced titer. In dogs, 1.2% showed nAbs against Omicron BA.1. These results indicate that nAbs were more frequent in cats than in dogs and that these nAbs have a lower capacity to neutralize the subvariant Omicron BA.1.

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Section: Discussionmentioning

confidence: 99%

Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico

Dehesa-Canseco,

Pastrana-Unzueta,

Carrillo-Guzmán

et al. 2023

Pathogens

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show abstract

“…Conversely, poultry such as chicken, ducks, turkey, quail, and goose are not susceptible to SARS-CoV infection. In contrast, pigs and cattle have extremely low susceptibility to SARS-CoV infection [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Further, two cases of mild respiratory disease in two cats were described in New York State, where, despite the positive detection of SARS-CoV-2 in the affected cats, the source of infection was only determined in one of the cases related to the infected owner [21]. Subsequently, sporadic detections of SARS-CoV-2 infections in cats showing respiratory and gastrointestinal clinical signs associated with COVID-19positive owners were reported worldwide [11,[22][23][24][25][26]. Thus, these case studies evidence that cats from affected households are at higher risk of infection compared to those without exposure to SARS-CoV-2.…”

Section: Introductionmentioning

confidence: 99%

Assessing the Potential Role of Cats (Felis catus) as Generators of Relevant SARS-CoV-2 Lineages during the Pandemic

Gomez-Romero,

Basurto-Alcantara,

Velazquez-Salinas

2023

Pathogens

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Several questions regarding the evolution of SARS-CoV-2 remain poorly elucidated. One of these questions is the possible evolutionary impact of SARS-CoV-2 after the infection in domestic animals. In this study, we aimed to evaluate the potential role of cats as generators of relevant SARS-CoV-2 lineages during the pandemic. A total of 105 full-length genome viral sequences obtained from naturally infected cats during the pandemic were evaluated by distinct evolutionary algorithms. Analyses were enhanced, including a set of highly related SARS-CoV-2 sequences recovered from human populations. Our results showed the apparent high susceptibility of cats to the infection SARS-CoV-2 compared with other animal species. Evolutionary analyses indicated that the phylogenomic characteristics displayed by cat populations were influenced by the dominance of specific SARS-CoV-2 genetic groups affecting human populations. However, disparate dN/dS rates at some genes between populations recovered from cats and humans suggested that infection in these two species may suggest a different evolutionary constraint for SARS-CoV-2. Interestingly, the branch selection analysis showed evidence of the potential role of natural selection in the emergence of five distinct cat lineages during the pandemic. Although these lineages were apparently irrelevant to public health during the pandemic, our results suggested that additional studies are needed to understand the role of other animal species in the evolution of SARS-CoV-2 during the pandemic.

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Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil

Galhardo,

Barbosa,

Kmetiuk

et al. 2023

Sci Rep

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Despite previous reports of SARS-CoV-2 infection in dogs and cats worldwide, the type of swab sample used for its detection through RT-qPCR needs to be better compared and described. Accordingly, as part of a multicenter study in Brazil, the aim of the present study was to assess which rectal or oropharyngeal swabs would be more appropriate for detecting SARS-CoV-2 in cats and dogs, through viral load comparison. Pets of owners diagnosed with COVID-19 in the last 7 days were eligible. A total of 148 animals from four of the five Brazilian geographical regions were analyzed, among which 10/48 cats (20.83%) and 11/100 dogs (11.00%) were positive. The results suggested that oropharyngeal swabs should be considered for SARS-CoV-2 detection, particularly in cats, due to the higher cDNA viral load. Also, the genomic results showed similarities between SARS-CoV-2 animal variants and human variants that were circulating at the time of sampling, thus corroborating the existence of zooanthroponotic transmission. In conclusion, the present study highlighted the importance of SARS-CoV-2 monitoring among cats and dogs, as virus modification may indicate the possibility of mutations in animals and spillover back to owners. Thus, positive individuals should always self-isolate from their pets during COVID-19, to prevent trans-species transmission and mutation.

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Near-Full-Length Genome Sequences Representing an Event of Zooanthroponotic Transmission of SARS-CoV-2 Lineage B.1.189 in Mexico during 2020

Cited by 4 publications

References 7 publications

Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico

Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico

Assessing the Potential Role of Cats (Felis catus) as Generators of Relevant SARS-CoV-2 Lineages during the Pandemic

Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil

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