Near-fixation of a Pfmsp1 block 2 allelic variant in genetically diverse Plasmodium falciparum populations across Western Colombia

Jiménez, J. Natalia; Snounou, Georges; Letourneur, Frank; Rénia, Laurent; Vélez, Iván Darío; Muskus, Carlos

doi:10.1016/j.actatropica.2009.12.009

Cited by 8 publications

(7 citation statements)

References 14 publications

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“…These findings are similar to previous studies in Iran, Pakistan, Myanmar, Bangladesh and Colombia which demonstrated the predominance of the MAD20 [7,16,17,19,20]. By contrast, previous studies in Lao PDR, Zambia, Gabon, Congo and other African countries showed that K1 was the predominant allelic family [21][22][23][24]. Monomorphic band of RO33 allelic types in Iran were observed on agarose gel electrophoresis.…”

Section: Discussionsupporting

confidence: 66%

Genetic Diversity of Variable Region Block 2 in the Merozoite Surface Protein-1 (MSP1) in Plasmodium falciparum Field Isolates from South-East of Iran

Tahareh¹

2014

Malaria Contr Elimination

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Merozoite surface protein 1 of (PfMSP-1) is a leading malaria vaccine candidate. However, extensive genetic diversity of this gene in field isolates of represents a major obstacle for the development of an effective vaccine against malaria. The present study was aimed at analysing genetic polymorphisms of K1, MAD20 and RO33 allelic types of MSP-1 block 2 among isolates from Sistan and Baluchestan, Iran.In this study a total of 94 infected persons from Sistan and Baluchistan Province of Iran, were included. Blood samples were collected from March 2011 to September 2012. Block 2 of the MSP-1 gene was genotyped by allele-specific nested polymerase chain reaction (PCR) after DNA extraction. Eighty-nine (94.7%) of the 94 samples were successfully amplified; 7 distinct MSP-1 genotypes were identified by size differences on agarose gels. MAD20 was the predominant MSP-1 allelic family identified in 46.1% (41/89) of the samples while RO33 family had the least frequency (7.9%). A total of 9/89 (10.1%) samples exhibited multiple infections with two alleles at PfMSP-1. The present study shows that the level of genetic diversity is relatively low in south-east of Iran and most of infections are composed of one clone, which is consistent with an area of low malaria transmission. These data are useful for malaria prevention and control in Iran.

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Section: Discussionsupporting

confidence: 66%

Genetic Diversity of Variable Region Block 2 in the Merozoite Surface Protein-1 (MSP1) in Plasmodium falciparum Field Isolates from South-East of Iran

Tahareh¹

2014

Malaria Contr Elimination

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“…In this study, all three allelic families of MSP-1 (K1, MAD20 and RO33) were observed; however, MAD20 was the predominant allelic family. In similar studies in Iran, Pakistan, Myanmar, Bangladesh and Colombia, MAD20 family also had the highest frequency (6,16,17,19,20), but K1 was the predominant allelic family in studies conducted in Laos, Zambia, Gabon, Congo and other African countries (21)(22)(23)(24). In this study, monomorphic bands in electrophoresis of the RO33 allele family were observed on agarose gel.…”

Section: Discussionsupporting

confidence: 49%

Genetic Diversity Block 2 of Surface Protein-1 in Plasmodium Falciparum Merozoite by Nested-PCR Method in Southeastern Iran

2015

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Background and Objectives: Plasmodium falciparum merozoite surface protein-1 (PfMSP-1) is a promising vaccine against malaria during its blood stages which play an important role in immunity to this disease. Polymorphic nature of this gene is a major obstacle in making an effective vaccine against malaria. In this study, the genetic diversity of Plasmodium falciparum isolates was investigated in Sistan-Baluchestan Province using allelic families of the MSP-1. Methods: From March/April 2011 to August/September 2012, 94 blood samples were collected from patients with falciparum malaria who were living in four districts of Sistan-Baluchestan Province. The extracted genomic DNA and genetic diversity of MSP-1 block 2 were evaluated by nested polymerase chain reaction. Results: From a total of 94 patients, 89 patients (94.7%) had positive PCR results and the remaining five patients were excluded. Seven different alleles of MSP-1 were identified through size difference on agarose gel. Comprising 46.1% of the samples, MAD20 was identified as the predominant MSP-1 allelic family, while the RO33 family had the lowest frequency (with 7.9%). In 10% of samples infection with two alleles was observed. Conclusion: The results of this study suggest that genetic diversity of PfMSP-1 in Southeastern Iran is relatively low and most infections originate from a clone that is consistent with an area of low malaria transmission. This information is useful for the prevention and control of malaria in Iran.

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“…In two Latin American countries (Peru and Brazil), K1 shows a slight predominance over MAD20, while the frequency of RO33 is only marginal. Similarly, a study carried out with samples from Colombia reports an almost absolute predominance of MAD20 and did not report the presence of RO33 [37]. It should be noted then that the frequency of RO33 in Central America has increased compared to previous reports, resembling the results of similar studies carried out in Africa and Asia [29,38].…”

Section: Discussionmentioning

confidence: 66%

Evidence of a Recent Bottleneck in Plasmodium falciparum Populations on the Honduran–Nicaraguan Border

et al. 2021

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The countries of Central America and the island of Hispaniola have set the goal of eliminating malaria in less than a decade. Although efforts to reduce the malaria burden in the region have been successful, there has been an alarming increase in cases in the Nicaraguan Moskitia since 2014. The continuous decrease in cases between 2000 and 2014, followed by a rapid expansion from 2015 to the present, has generated a potential bottleneck effect in the populations of Plasmodium spp. Consequently, this study aimed to evaluate the genetic diversity of P. falciparum and the decrease in allelic richness in this population. The polymorphic regions of the pfmsp-1 and pfmsp-2 genes of patients with falciparum malaria from Honduras and Nicaragua were analyzed using nested PCR and sequencing. Most of the samples were classified into the K1 allelic subfamily of the pfmsp-1 gene and into the 3D7 subfamily of the pfmsp-2 gene. Despite the low genetic diversity found, more than half of the samples presented a polyclonal K1/RO33 haplotype. No sequence polymorphisms were found within each allelic subfamily. This study describes a notable decrease in the genetic diversity of P. falciparum in the Moskitia region after a bottleneck phenomenon. These results will be useful for future epidemiological investigations and the monitoring of malaria transmission in Central America.

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Near-fixation of a Pfmsp1 block 2 allelic variant in genetically diverse Plasmodium falciparum populations across Western Colombia

Cited by 8 publications

References 14 publications

Genetic Diversity of Variable Region Block 2 in the Merozoite Surface Protein-1 (MSP1) in Plasmodium falciparum Field Isolates from South-East of Iran

Genetic Diversity of Variable Region Block 2 in the Merozoite Surface Protein-1 (MSP1) in Plasmodium falciparum Field Isolates from South-East of Iran

Genetic Diversity Block 2 of Surface Protein-1 in Plasmodium Falciparum Merozoite by Nested-PCR Method in Southeastern Iran

Evidence of a Recent Bottleneck in Plasmodium falciparum Populations on the Honduran–Nicaraguan Border

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