Near-field sensor array with 65-GHz CMOS oscillators for rapid detection of viable Escherichia coli

Ogawa, Yuichi; Kikuchi, Shojiro; Yamashige, Yoshihisa; Shiraga, Keiichiro; Mitsunaka, Takeshi

doi:10.1016/j.bios.2020.112935

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…1E), the fluidity of the liquid medium allowed the BCG to mix and adhere to each other, forming a bulky mass, and Sensing bacterial growth and response to drugs. The 65-GHz frequency band used in our sensor is sensitive to changes in the number of bulk water molecules but is largely unaffected by larger molecules and ions 18,21 . When bacteria in the liquid medium settled onto the surface of the sensor, the main factors affecting the difference in resonance frequency (MHz) were: (1) the total bacterial volume, (2) the number of bulk water molecules, and (3) changes in the dielectric constant owing to bacterial metabolites.…”

Section: Resultsmentioning

confidence: 99%

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Near-field sensor array with 65-GHz CMOS oscillators can rapidly and comprehensively evaluate drug susceptibility of Mycobacterium

Kikuchi¹,

Yamashige²,

Hosoki³

et al. 2023

Sci Rep

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Multidrug-resistant tuberculosis (MDR-TB) is a major clinical problem. Because Mycobacterium, the causative agent of tuberculosis, are slow-growing bacteria, it takes 6–8 weeks to complete drug susceptibility testing, and this delay contributes to the development of MDR-TB. Real-time drug resistance monitoring technology would be effective for suppressing the development of MDR-TB. In the electromagnetic frequency from GHz to THz regions, the spectrum of the dielectric response of biological samples has a high dielectric constant owing to the relaxation of the orientation of the overwhelmingly contained water molecule network. By measuring the change in dielectric constant in this frequency band in a micro-liquid culture of Mycobacterium, the growth ability can be detected from the quantitative fluctuation of bulk water. The 65-GHz near-field sensor array enables a real-time assessment of the drug susceptibility and growth ability of Mycobacterium bovis (BCG). We propose the application of this technology as a potential new method for MDR-TB testing.

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Section: Resultsmentioning

confidence: 99%

“…This sensor system can quickly determine the growth of Escherichia coli and the action of streptomycin (SM) 21 . This system was able to recognize cell death with higher sensitivity than the fluorescence imaging method by measuring the dielectric constant of HeLa cells in culture over time 22 .…”

mentioning

confidence: 99%

Near-field sensor array with 65-GHz CMOS oscillators can rapidly and comprehensively evaluate drug susceptibility of Mycobacterium

Kikuchi¹,

Yamashige²,

Hosoki³

et al. 2023

Sci Rep

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“…271 Additionally, Ogawa and colleagues successfully differentiated between live E. coli and heat-treated E. coli within 20 min utilizing a near-field sensor array. 272…”

Section: Biosensor-based Assaysmentioning

confidence: 99%

Progress in methods for the detection of viable Escherichia coli

Zhuang,

Gong,

Zhao

et al. 2024

Analyst

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“…Their device was used to characterize the current generating capacity of exoelectrogenic bacteria, but the pixel size (100 μm x 100 μm) did not provide single-cell resolution. Ogawa et al demonstrated an array of high frequency oscillators for monitoring the growth of E. coli (Ogawa et al, 2021). The small pixel area needed for single-cell bacteria measurements allows only limited space for in-pixel electronics necessary to amplify the small sensing currents (Niitsu et al, 2015).…”

Section: Electrical Techniquesmentioning

confidence: 99%

Opportunities in optical and electrical single-cell technologies to study microbial ecosystems

Mermans,

Mattelin,

Van den Eeckhoudt

et al. 2023

Front. Microbiol.

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New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Raman spectroscopy and emerging electrical techniques. The objectives of this review include showcasing the diversity of single-cell optical approaches for studying microbiological phenomena, highlighting successful applications in understanding microbial systems, discussing emerging techniques, and encouraging the combination of established and novel approaches to address research questions. The review aims to answer key questions such as how single-cell approaches have advanced our understanding of individual and interacting cells, how they have been used to study uncultured microbes, which new analysis tools will become widespread, and how they contribute to our knowledge of ecological interactions.

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Near-field sensor array with 65-GHz CMOS oscillators for rapid detection of viable Escherichia coli

Cited by 8 publications

References 30 publications

Near-field sensor array with 65-GHz CMOS oscillators can rapidly and comprehensively evaluate drug susceptibility of Mycobacterium

Near-field sensor array with 65-GHz CMOS oscillators can rapidly and comprehensively evaluate drug susceptibility of Mycobacterium

Progress in methods for the detection of viable Escherichia coli

Opportunities in optical and electrical single-cell technologies to study microbial ecosystems

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