Near-Complete Whole-Genome Sequencing of Two Burkholderia pseudomallei Strains Harbouring Novel Molecular Class D Beta-Lactamase Genes, Isolated from Malaysia

Abstract

Here, we present two 7.1- and 7.3-bp near-complete genome sequences of Burkholderia pseudomallei strains of HTAA077 and HRPB058, isolated from a pus culture from a confirmed melioidosis patient at Hospital Tengku Ampuan Afzan, Kuantan, Malaysia, and from blood culture from a patient at Hospital Raja Permaisuri Bainun, Ipoh, Malaysia, using a Nanopore MinION instrument.

Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing

“…This work confirms the high importance for bacterial pathogens detection due to their various uses, as reported before [ 1 , 2 , 7 , [25] , [26] , [27] , [28] , [29] , [30] , [31] ].…”

Whole-Genome Sequence of a Stenotrophomonas maltophilia Isolate from Tap Water in an Intensive Care Unit

“…A high-sensitivity kit (DeNovix, Wilmington, DE) and agarose gel electrophoresis were used to evaluate the concentration and quality of the gDNA, respectively. Prior to library preparation, DNA was fragmented to an average length of >6 kb using SPRIselect reagent (Beckman Coulter Inc., Indianapolis, IN, USA) as described previously ( 4 ). Briefly, 1.25 μg of DNA was used for library preparation with a ligation sequencing kit (SQK-LSK110; Oxford Nanopore Technologies, Oxford, UK).…”