Near-Complete Genome Sequences of New Strains of
            <i>Nylanderia Fulva Virus 1</i>
            from Solenopsis invicta

Allen, Margaret L.

doi:10.1128/mra.00798-19

Cited by 3 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multiple RNA viruses have been shown to be transmitted from managed honeybees to other pollinating insects as well as arthropods living in close contact with honeybees or sharing the same environmental resources [ 59 , 60 ]. To date, only two case studies involving potential ant virus spillover have been reported: (1) Nylanderia fulva virus 1, an RNA virus infecting the tawny crazy ant ( Nylanderia fulva ), was found infecting the red imported fire ant [ 61 ], and (2) Solenopsis invicta virus 4 (SINV-4) infecting the red imported fire ant was found in multiple co-existing ant species (although no evidence of active replication was found) [ 62 ]. These findings indicate the possibility of viral infection in a non-host ant.…”

Section: Discussionmentioning

confidence: 99%

First Polycipivirus and Unmapped RNA Virus Diversity in the Yellow Crazy Ant, Anoplolepis gracilipes

Lee

Hsu

Lin

et al. 2022

Viruses

View full text Add to dashboard Cite

The yellow crazy ant, Anoplolepis gracilipes is a widespread invasive ant that poses significant threats to local biodiversity. Yet, compared to other global invasive ant species such as the red imported fire ant (Solenopsis invicta) or the Argentine ant (Linepithema humile), little is known about the diversity of RNA viruses in the yellow crazy ant. In the current study, we generated a transcriptomic database for A. gracilipes using a high throughput sequencing approach to identify new RNA viruses and characterize their genomes. Four virus species assigned to Dicistroviridae, two to Iflaviridae, one to Polycipiviridae, and two unclassified Riboviria viruses were identified. Detailed genomic characterization was carried out on the polycipivirus and revealed that this virus comprises 11,644 nucleotides with six open reading frames. Phylogenetic analysis and pairwise amino acid identity comparison classified this virus into the genus Sopolycivirus under Polycipiviridae, which is tentatively named “Anoplolepis gracilipes virus 3 (AgrV-3)”. Evolutionary analysis showed that AgrV-3 possesses a high level of genetic diversity and elevated mutation rate, combined with the common presence of multiple viral strains within single worker individuals, suggesting AgrV-3 likely evolves following the quasispecies model. A subsequent field survey placed the viral pathogen “hotspot” of A. gracilipes in the Southeast Asian region, a pattern consistent with the region being recognized as part of the ant’s native range. Lastly, infection of multiple virus species seems prevalent across field colonies and may have been linked to the ant’s social organization.

show abstract

Section: Discussionmentioning

confidence: 99%

First Polycipivirus and Unmapped RNA Virus Diversity in the Yellow Crazy Ant, Anoplolepis gracilipes

Lee

Hsu

Lin

et al. 2022

Viruses

View full text Add to dashboard Cite

show abstract

“…To date, the S. invicta virome is composed of mainly positive-sense single-strand RNA (+ ssRNA) viruses in the order Picornavirales . These include eleven viruses from families Dicistroviridae , Polycipiviridae , Iflaviridae , Soliniviridae and two unclassified viruses [ 11 , 31 ]. Additionally, one double-strand RNA (dsRNA) and one single-strand DNA (ssDNA) of the families Totiviridae and Parvoviridae , respectively, have been characterized [ 11 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, one double-strand RNA (dsRNA) and one single-strand DNA (ssDNA) of the families Totiviridae and Parvoviridae , respectively, have been characterized [ 11 , 32 ]. While most of these viruses have been reported associated with S. invicta in its native range, only the species Solenopsis invicta virus 1 , Solenopsis invicta virus 2 , Solenopsis invicta virus 3 , Solenopsis invicta virus 6 and Nylanderia fulva virus 1 have been reported in the United States [ 11 , 31 ]. Although great progress has been reached in identification and molecular characterization of the S. invicta virome, the effect of these viruses and their potential use in biological control will require additional investigation.…”

Section: Introductionmentioning

confidence: 99%

Ever-increasing viral diversity associated with the red imported fire ant Solenopsis invicta (Formicidae: Hymenoptera)

Xavier

Allen

Whitfield

2021

Virol J

View full text Add to dashboard Cite

Background Advances in sequencing and analysis tools have facilitated discovery of many new viruses from invertebrates, including ants. Solenopsis invicta is an invasive ant that has quickly spread worldwide causing significant ecological and economic impacts. Its virome has begun to be characterized pertaining to potential use of viruses as natural enemies. Although the S. invicta virome is the best characterized among ants, most studies have been performed in its native range, with less information from invaded areas. Methods Using a metatranscriptome approach, we further identified and molecularly characterized virus sequences associated with S. invicta, in two introduced areas, U.S and Taiwan. The data set used here was obtained from different stages (larvae, pupa, and adults) of S. invicta life cycle. Publicly available RNA sequences from GenBank’s Sequence Read Archive were downloaded and de novo assembled using CLC Genomics Workbench 20.0.1. Contigs were compared against the non-redundant protein sequences and those showing similarity to viral sequences were further analyzed. Results We characterized five putative new viruses associated with S. invicta transcriptomes. Sequence comparisons revealed extensive divergence across ORFs and genomic regions with most of them sharing less than 40% amino acid identity with those closest homologous sequences previously characterized. The first negative-sense single-stranded RNA virus genomic sequences included in the orders Bunyavirales and Mononegavirales are reported. In addition, two positive single-strand virus genome sequences and one single strand DNA virus genome sequence were also identified. While the presence of a putative tenuivirus associated with S. invicta was previously suggested to be a contamination, here we characterized and present strong evidence that Solenopsis invicta virus 14 (SINV-14) is a tenui-like virus that has a long-term association with the ant. Furthermore, based on virus sequence abundance compared to housekeeping genes, phylogenetic relationships, and completeness of viral coding sequences, our results suggest that four of five virus sequences reported, those being SINV-14, SINV-15, SINV-16 and SINV-17, may be associated to viruses actively replicating in the ant S. invicta. Conclusions The present study expands our knowledge about viral diversity associated with S. invicta in introduced areas with potential to be used as biological control agents, which will require further biological characterization.

show abstract

“…To date, the S. invicta virome is composed of mainly positive-sense single-strand RNA (+ssRNA) viruses in the order Picornavirales. These include eleven viruses from families Dicistroviridae, Polycipiviridae, Iflaviridae, Soliniviridae and two unclassified viruses (11,31). Additionally, one double-strand RNA (dsRNA) and one single-strand DNA (ssDNA) of the families Totiviridae and Parvoviridae, respectively, have been characterized (11,32).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, one double-strand RNA (dsRNA) and one single-strand DNA (ssDNA) of the families Totiviridae and Parvoviridae, respectively, have been characterized (11,32). While most of these viruses have been reported associated with S. invicta in its native range, only the species Solenopsis invicta virus 1 (SINV-1), SINV-2, SINV-3, SINV-6 and Nylanderia fulva virus 1 (NfV-1) have been reported in United States (11,31). Although great progress has been reached in identification and molecular characterization of S. invicta virome, the effect of these viruses and their potential use in biological control still need to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Ever-increasing viral diversity associated with the red imported fire ant Solenopsis invicta (Formicidae: Hymenoptera)

Xavier

Allen

Whitfield

2020

Preprint

Self Cite

View full text Add to dashboard Cite

BackgroundAdvances in sequencing and analysis tools have facilitated discovery of many new viruses from invertebrates, including ants. Solenopsis invicta is an invasive ant that has quickly spread around world causing significant ecological and economic impacts. Its virome has begun to be characterized pertaining to potential use of viruses as natural enemies. Although the S. invicta virome is best characterized among ants, most studies have been performed in its native range, with little information from invaded areas.MethodsUsing a metatranscriptome approach, we further characterized viruses associated with S. invicta, in two introduced areas, U.S and Taiwan. The data set used here was obtained from different stages (larvae, pupa, and adults) of S. invicta life cycle. Publicly available RNA sequences from GenBank’s Sequence Read Archive were downloaded and de novo assembled using CLC Genomics Workbench 20.0.1. Contigs were compared against the non-redundant protein sequences and those showing similarity to viral sequences were further analyzed.ResultsWe characterized five putative new viruses associated with S. invicta transcriptomes. Sequence comparisons revealed extensive divergence across ORFs and genomic regions with most of them sharing less than 40% amino acid identity with those closest homologous sequences previously characterized. The first negative-sense single-stranded RNA viruses included in the orders Bunyavirales and Mononegavirales are reported. In addition, two positive single-strand viruses and one single strand DNA virus were also characterized. While the presence of a putative tenuivirus associated with S. invicta was previously suggested to be a contamination, here we characterized and present strong evidence that Solenopsis invicta virus 14 (SINV-14) is a tenui-like virus that has a long-term association with the ant. Furthermore, based on virus abundance compared to housekeeping genes, phylogenetic relationships, and completeness of viral coding sequences, our results suggest that four of five viruses reported, those being SINV-14, SINV-15, SINV-16 and SINV-17, replicate in the ant S. invicta.ConclusionsThe present study expands our knowledge about viral diversity associated with S. invicta in introduced areas with potential to be used as biological control agents, which will require further biological characterization.

show abstract

Near-Complete Genome Sequences of New Strains of Nylanderia Fulva Virus 1 from Solenopsis invicta

Cited by 3 publications

References 15 publications

First Polycipivirus and Unmapped RNA Virus Diversity in the Yellow Crazy Ant, Anoplolepis gracilipes

First Polycipivirus and Unmapped RNA Virus Diversity in the Yellow Crazy Ant, Anoplolepis gracilipes

Ever-increasing viral diversity associated with the red imported fire ant Solenopsis invicta (Formicidae: Hymenoptera)

Ever-increasing viral diversity associated with the red imported fire ant Solenopsis invicta (Formicidae: Hymenoptera)

Contact Info

Product

Resources

About