NDRG4 promotes myogenesis via Akt/CREB activation

Zhu, Mingfei; Zheng, Rong; Guo, Yiwen; Zhang, Yunxia; Zuo, Bo

doi:10.18632/oncotarget.21591

Cited by 14 publications

(9 citation statements)

References 55 publications

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“…Insulin activates Akt/ mTOR/S6K signaling via upregulating PI3K, 30 and promotes myoblast proliferation and differentiation via mTOR-mediated MYOD and MYOG expression. 59 Thus, NRF enhances insulin secretion and downstream mTOR signaling, thereby promoting protein synthesis and muscle growth. Previous studies in adult animals have shown that time-restricted feeding can reduce body weight by increasing energy expenditure.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, this study revealed that NRF promotes the expression of mTOR, S6K, and MYOG. Insulin activates Akt/mTOR/S6K signaling via upregulating PI3K, 30 and promotes myoblast proliferation and differentiation via mTOR‐mediated MYOD and MYOG expression 59 . Thus, NRF enhances insulin secretion and downstream mTOR signaling, thereby promoting protein synthesis and muscle growth.…”

Section: Discussionmentioning

confidence: 99%

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Night‐restricted feeding improves locomotor activity rhythm and modulates nutrient utilization to accelerate growth in rabbits

et al. 2020

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An unfavorable lifestyle disrupts the circadian rhythm, leading to metabolic dysfunction in adult humans and animals. Increasing evidence suggests that night-restricted feeding (NRF) can effectively prevent ectopic fat deposition caused by circadian rhythm disruption, and reduce the risk of metabolic diseases. However, previous studies have mainly focused on the prevention of obesity in adults by regulating dietary patterns, whereas limited attention has been paid to the effect of NRF on metabolism during growth and development. Here, we used weaning rabbits as models and found that NRF increased body weight gain without increasing feed intake, and promoted insulin-mediated protein synthesis through the mTOR/S6K pathway and muscle formation by upregulating MYOG. NRF improved the circadian clock, promoted PDH-regulated glycolysis and CPT1B-regulated fatty-acid β-oxidation, and reduced fat content in the serum and muscles. In addition, NRF-induced body temperature oscillation might be partly responsible for the improvement in the circadian clock and insulin sensitivity. Time-restricted feeding could be used as a nondrug intervention to prevent obesity and accelerate growth in adolescents. K E Y W O R D S adolescent obesity, circadian rhythm, core body temperature, muscle growth, night-restricted feeding 2 of 13 | GUO et al.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Night‐restricted feeding improves locomotor activity rhythm and modulates nutrient utilization to accelerate growth in rabbits

et al. 2020

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“…Among the others, NDRG4 (NDRG Family Member 4) and COQ9 (Coenzyme Q9) genes deserve particular mention. Indeed, NDRG4 plays a key role in regulating the myogenic differentiation via Akt/CREB activation [82] whereas COQ9 was reported in association with water holding capacity and with several quality traits in pigs [83]. PRKACB (Protein Kinase CAMP-Activated Catalytic Subunit Beta) gene is intriguing considering the role played by cAMP-dependent protein kinase in skeletal muscle adaptation [84].…”

Section: Discussionmentioning

confidence: 99%

Single-Step Genome Wide Association Study Identifies QTL Signals for Untrimmed and Trimmed Thigh Weight in Italian Crossbred Pigs for Dry-Cured Ham Production

Palombo

D’Andrea

Licastro

et al. 2021

Animals

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Protected Designation of Origin (PDO) dry-cured ham is the most important product in the Italian pig breeding industry, mainly oriented to produce heavy pig carcasses to obtain hams of the right weight and maturity. Recently, along with the traditional traits swine breeding programs have aimed to include novel carcass traits. The identification at the genome level of quantitative trait loci (QTLs) affecting such new traits helps to reveal their genetic determinism and may provide information to be integrated in prediction models in order to improve prediction accuracy as well as to identify candidate genes underlying such traits. This study aimed to estimate genetic parameters and perform a single step genome wide association studies (ssGWAS) on novel carcass traits such as untrimmed (UTW) and trimmed thigh weight (TTW) in two pig crossbred lines approved for the ham production of the Italian PDO. With this purpose, phenotypes were collected from ~1800 animals and 240 pigs were genotyped with Illumina PorcineSNP60 Beadchip. The single-step genomic BLUP procedure was used for the heritability estimation and to implement the ssGWAS. QTL were characterized based on the variance of 10-SNP sliding window genomic estimated breeding values. Moderate heritabilities were detected and QTL signals were identified on chromosome 1, 4, 6, 7, 11 and 15 for both traits. As expected, the genetic correlation among the two traits was very high (~0.99). The QTL regions encompassed a total of 249 unique candidate genes, some of which were already reported in association with growth, carcass or ham weight traits in pigs. Although independent studies are required to further verify our findings and disentangle the possible effects of specific linkage disequilibrium in our population, our results support the potential use of such new QTL information in future breeding programs to improve the reliability of genomic prediction.

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“…Normally, Pax 7 expression on satellite cells decreases whilst the MyoD increases culminating with myoblast activation, proliferation, fusion and differentiation (Sambasivan et al, 2011). Indeed, the MRP process requires a sequence of phosphorylation events, including Akt and cAMP response element binding protein (CREB) residues, that are linked to promote MyoD expression and consequently myoblast differentiation (Stewart et al, 2011;Zhu et al, 2017). Others have shown that pCREB interacts with MyoD (Magenta et al, 2003) and CREB with Akt (Du and Montminy, 1998).…”

Section: Discussionmentioning

confidence: 99%

Endogenous Galectin-3 is Required for Skeletal Muscle Repair

Cerri

Rodrigues

Alves

et al. 2020

Preprint

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Skeletal muscle has the intrinsic ability to self-repair through a multifactorial process, but many aspects of its cellular and molecular mechanisms are not fully understood. There is increasing evidence that some members of the mammalian β-galactoside-binding protein family (galectins) are involved in the muscular repair process (MRP), including galectin-3 (Gal-3). However, there are many questions about the role of this protein on muscle self-repair. Here, we demonstrate that endogenous Gal-3 is required for: i) muscle repair in vivo using a chloride-barium myolesion mouse model, and ii) mouse primary myoblasts myogenic programming. Injured muscle from Gal-3 knockout mice (GAL3KO) showed persistent inflammation associated with compromised muscle repair and the formation of fibrotic tissue on the lesion site. In GAL3KO mice, osteopontin expression remained high even after 7 and 14 days of the myolesion, while MyoD and myogenin had decreased their expression. In GAL3KO mouse primary myoblast cell culture, Pax7 detection seems to sustain even when cells are stimulated to differentiation and MyoD expression is drastically reduced. These findings suggest that the detection and temporal expression levels of these transcriptional factors appear to be altered in Gal-3-deficient myoblast cell culture compared to Wild Type (WT) cells. We observed Gal-3 expression in WT states, both in vivo and in vitro, in sarcoplasm/cytoplasm and myonuclei; as differentiation proceeds, Gal-3 expression is drastically reduced, and its location is confined to the sarcolemma/plasma cell membrane. We also observed a change in the temporal-spatial profile of Gal-3 expression and muscle transcription factors levels during the myolesion. Overall, these results demonstrate that endogenous Gal-3 is required for the skeletal muscle repair process.

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NDRG4 promotes myogenesis via Akt/CREB activation

Cited by 14 publications

References 55 publications

Night‐restricted feeding improves locomotor activity rhythm and modulates nutrient utilization to accelerate growth in rabbits

Night‐restricted feeding improves locomotor activity rhythm and modulates nutrient utilization to accelerate growth in rabbits

Single-Step Genome Wide Association Study Identifies QTL Signals for Untrimmed and Trimmed Thigh Weight in Italian Crossbred Pigs for Dry-Cured Ham Production

Endogenous Galectin-3 is Required for Skeletal Muscle Repair

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