NDM-5-producing Escherichia coli in an urban river in Montpellier, France

Almakki, Ayad; Maure, Alexandra; Pantel, Alix; Romano-Bertrand, Sara; Masnou, Agnès; Marchandin, Hélène; Jumas‐Bilak, Estelle; Licznar-Fajardo, Patricia

doi:10.1016/j.ijantimicag.2017.04.003

Cited by 20 publications

(20 citation statements)

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“…Furthermore, NDM-5-positive strains were not only isolated from clinical specimens but also from animals, such as dogs [ 9 ], cats [ 10 ] and cows [ 11 ]. Worryingly, bla NDM-5 has also been identified in environmental samples [hospital sewage water [ 12 ] and urban river [ 13 ]], indicating its presence in the community. However, the dissemination mechanism of bla NDM-5 gene remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Dissemination of blaNDM-5 gene via an IncX3-type plasmid among non-clonal Escherichia coli in China

Shen

et al. 2018

Antimicrob Resist Infect Control

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BackgroundThe emergence and spread of New Delhi metallo-β-lactamase-producing Enterobacteriaceae has been a serious challenge to manage in the clinic due to its rapid dissemination of multi-drug resistance worldwide. As one main type of carbapenemases, New Delhi metallo-β-lactamase (NDM)is able to confer resistance to almost all β-lactams, including carbapenems, in Enterobacteriaceae. Recently, New Delhi metallo-β-lactamase-5 attracted extensive attention because of increased resistance to carbapenems and widespread dissemination. However, the dissemination mechanism of blaNDM-5 gene remains unclear.MethodsA total of 224 carbapenem-resistant Enterobacteriaceae isolates (CRE) were collected from different hospitals in Zhejiang province. NDM-5-positive isolates were identified and subjected to genotyping, susceptibility testing, and clinical data analysis. We established the genetic location of blaNDM-5 with southern blot hybridisation, and analysed plasmids containing blaNDM-5 with filter mating and DNA sequencing.ResultsEleven New Delhi metallo-β-lactamase-5 (NDM-5)-producing strains were identified, including 9 Escherichia coli strains, 1 Klebsiella pneumoniae strain, and 1 Citrobacter freundii strain. No epidemiological links for E. coli isolates were identified by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). S1-PFGE and southern blot suggested that the blaNDM-5 gene was located on a 46-kb IncX3-type plasmid in all isolates. Nine of the 11 isolates (81.8%) tested could successfully transfer their carbapenem-resistant phenotype to E. coli strain C600. Moreover, sequence analysis further showed that this plasmid possessed high sequence similarity to most of previously reported blaNDM-5-habouring plasmids in China.ConclusionThe present data in this study showed the IncX3 type plasmid played an important role in the dissemination of blaNDM-5 in Enterobacteriaceae. In addition, to the best of our knowledge, this report is the first to isolate both E. coli and C. freundii strains carrying blaNDM-5 from one single patient, which further indicated the possibility of blaNDM-5 transmission among diverse species. Close surveillance is urgently needed to monitor the further dissemination of NDM-5-producing isolates.Electronic supplementary materialThe online version of this article (10.1186/s13756-018-0349-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Dissemination of blaNDM-5 gene via an IncX3-type plasmid among non-clonal Escherichia coli in China

Shen

et al. 2018

Antimicrob Resist Infect Control

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“…In spite of using the antibiotics and their presence in the environment, bacteria resistant to β-lactams are thriving and there are a constant presence in more and more countries [50,51,52]. The same emerging resistance pattern it is also observed for bacteria producing carbapenemase [53,54,55]. Unfortunately, bacteria producing carbapenemase, cephalosporinase or oxacillinase exchange their ARG among them or with other bacteria from the environment, subsequently spreading the resistance to antibiotics to other bacterial communities and other distant geographical areas [56,57].…”

Section: Enzymatic Degradation Of Antibioticsmentioning

confidence: 99%

Biodegradation of Antibiotics: The Balance Between Good and Bad

Galaon

Banciu

Chiriac

et al. 2019

RJEEC

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The worldwide population rise corroborated with the raise of the health-care standards have generated an escalation of the antibiotic production and uncontrolled usage. The subsequent effects of this escalation have led to an increase of the antibiotic resistance rates, Romania is in the top of the EU countries regarding the antibiotic resistance rates, and to a continuous presence in the environment, including the aquatic environment. Unfortunately, the present design of the classical WWTPs is not optimized for the efficient removal of antibiotics since these compounds may have highly soluble and polar molecular structures. Instead, antibiotics removal using microorganisms could be an ecofriendly solution to this environmental issue, as long as their antibiotic degradation structures are not more toxic than the antibiotic itself. In the present review, we focus on the environmental presence and biodegradation of the most commonly used antibiotics as well as on their biodegradation, based on bacterial model, monitored by mass-spectrometric methods.

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“…from hospital sewage effluent [ 11 , 12 ] and different Enterobacterales species producing NDM-1, NDM-5 and NDM-7 enzymes were isolated from WWTP effluent [ 23 ]. Recently, two isolates of E. coli carrying bla NDM-5 gene onto IncX3-type plasmids were isolated from an urban river in the South of France [ 63 ].…”

Section: Carbapenemases: a Clinical Success Storymentioning

confidence: 99%

“…A number of studies reported the isolation of CPE from urban aquatic environments such as surface water nearby WWTP [ 23 ], rivers [ 13 , 42 , 63 , 78 , 79 , 80 , 81 , 82 , 83 ], lakes [ 15 , 18 , 41 ] or coastal water [ 15 , 18 ]. In most of cases, the origin of CPE is recognized or strongly suggested to come from anthropic contaminations.…”

Section: Presence Of Cpb In Aquatic Environments: a Simply Remnantmentioning

confidence: 99%

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The Hydric Environment: A Hub for Clinically Relevant Carbapenemase Encoding Genes

2020

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Carbapenems are β-lactams antimicrobials presenting a broad activity spectrum and are considered as last-resort antibiotic. Since the 2000s, carbapenemase producing Enterobacterales (CPE) have emerged and are been quickly globally spreading. The global dissemination of carbapenemase encoding genes (CEG) within clinical relevant bacteria is attributed in part to its location onto mobile genetic elements. During the last decade, carbapenemase producing bacteria have been isolated from non-human sources including the aquatic environment. Aquatic ecosystems are particularly impacted by anthropic activities, which conduce to a bidirectional exchange between aquatic environments and human beings and therefore the aquatic environment may constitute a hub for CPE and CEG. More recently, the isolation of autochtonous aquatic bacteria carrying acquired CEG have been reported and suggest that CEG exchange by horizontal gene transfer occurred between allochtonous and autochtonous bacteria. Hence, aquatic environment plays a central role in persistence, dissemination and emergence of CEG both within environmental ecosystem and human beings, and deserves to be studied with particular attention.

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NDM-5-producing Escherichia coli in an urban river in Montpellier, France

Cited by 20 publications

References 5 publications

Dissemination of blaNDM-5 gene via an IncX3-type plasmid among non-clonal Escherichia coli in China

Dissemination of blaNDM-5 gene via an IncX3-type plasmid among non-clonal Escherichia coli in China

Biodegradation of Antibiotics: The Balance Between Good and Bad

The Hydric Environment: A Hub for Clinically Relevant Carbapenemase Encoding Genes

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