NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae

Goh, Phuay-Yee; Kilmartin, John V.

doi:10.1016/0962-8924(93)90052-3

Cited by 21 publications

(47 citation statements)

References 30 publications

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“…The insert shows a half spindle ranging from the spindle pole body (spb) to the neck region (nr), as displayed in Fig. 3B, C. In accordance with light microscopic observations (Goh and Kilmartin 1993;Zeng et al 1999;Goshima and Yanagida 2000), we found gold labeling close to the spindle pole bodies as well as along microtubules of anaphase spindles. Such a staining pattern was also observed in telophase-arrested cdc15-2 cells (data not shown).…”

Section: Resultssupporting

confidence: 85%

“…Therefore Ndc10p is considered as one of the core components of budding yeast kinetochores, and homologous proteins have not been described in either Schizosaccharomyces pombe or in mammalian systems. To date, Ndc10p has been localized by light microscopy to structures that resemble the entire mitotic spindle (Goh and Kilmartin 1993;Zeng et al 1999;Goshima and Yanagida 2000). Interestingly, bilobed localization of kinetochore proteins on either side of the spindle midzone has been reported (Goshima and Yanagida 2000;He et al 2000), and this pattern was related to the existence of transient sister chromatid separation.…”

Section: Introductionmentioning

confidence: 95%

“…CDEIII binds CBF3, an M r 240,000 multiprotein complex consisting of Ndc10p, Cep3p, Ctf13p, and Skp1p (Lechner and Carbon 1991). CBF3 is an essential complex as its absence causes the loss of kinetochore function both in vivo and in vitro and the disruption of all interactions of kinetochore proteins with the centromere (Goh and Kilmartin 1993;Sorger et al 1994;Ortiz et al 1999). Apart from this complex, many other components play a role in kinetochore function (reviewed by Cheeseman et al 2002).…”

Section: Introductionmentioning

confidence: 96%

“…The NDC10 gene has been isolated in a visual screen for abnormal spindle morphology (Goh and Kilmartin 1993;Jiang et al 1993). The phenotype of ndc10-1 cells at restrictive temperature is detachment of chromosomes from the spindle, while DNA replication, spindle formation, anaphase spindle elongation, and cytokinesis continue essentially normally (Goh and Kilmartin 1993). Another allele of NDC10, ndc10-2, exhibits anaphase spindles in which DNA is associated with only one pole .…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study was to analyze the distribution of budding yeast kinetochores during mitosis by electron microscopy using Ndc10p as a primary target for immunolocalization. The NDC10 gene has been isolated in a visual screen for abnormal spindle morphology (Goh and Kilmartin 1993;Jiang et al 1993). The phenotype of ndc10-1 cells at restrictive temperature is detachment of chromosomes from the spindle, while DNA replication, spindle formation, anaphase spindle elongation, and cytokinesis continue essentially normally (Goh and Kilmartin 1993).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3-D modeling of mitotic spindles

Müller‐Reichert

Sassoon²,

O’Toole³

et al. 2003

Chromosoma

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Ndc10p is one of the DNA-binding constituents of the kinetochore in Saccharomyces cerevisiae but light microscopy analysis suggests that Ndc10p is not limited to kinetochore regions. We examined the localization of Ndc10p using immunoelectron microscopy and showed that Ndc10p is associated with spindle microtubules from S-phase through anaphase. By serial section reconstruction of mitotic spindles combined with immunogold detection, we showed that Ndc10p interacts with microtubules laterally as well as terminally. About 50% of the gold label in serial section reconstructions of short mitotic spindles was associated with the walls of spindle microtubules. Interaction of kinetochore components with microtubule walls was also shown for kinetochore protein Ndc80p. Our data suggest that at least a subset of kinetochore-associated protein is dispersed throughout the mitotic spindle.

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Section: Resultssupporting

confidence: 85%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3-D modeling of mitotic spindles

Müller‐Reichert

Sassoon²,

O’Toole³

et al. 2003

Chromosoma

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Genetic evidence for a role of phospholipase C at the budding yeast kinetochore

et al. 2003

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Chromosome segregation during mitosis requires kinetochores, specialized organelles that mediate chromosome attachment to spindle microtubules. We have shown previously that in budding yeast, Plc1p (phosphoinositide-specific phospholipase C) localizes to centromeric loci, associates with the kinetochore proteins Ndc10p and Cep3p, and affects the function of kinetochores. Deletion of PLC1 results in nocodazole sensitivity, mitotic delay, and a higher frequency of chromosome loss. We report here that despite the nocodazole sensitivity of plc1D cells, Plc1p is not required for the spindle checkpoint. However, plc1D cells require a functional BUB1/BUB3-dependent spindle checkpoint for viability. PLC1 displays strong genetic interactions with genes encoding components of the inner kinetochore, including NDC10, SKP1, MIF2, CEP1, CEP3, and CTF13. Furthermore, plc1D cells display alterations in chromatin structure in the core centromere. Chromatin immunoprecipitation experiments indicate that Plc1p localizes to centromeric loci independently of microtubules, and accumulates at the centromeres during G 2 /M stage of cell cycle. These results are consistent with the view that Plc1p affects kinetochore function, possibly by modulating the structure of centromeric chromatin.

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A change in sister chromatid behavior precedes nuclear division in Saccharomyces cerevisiae

Burke

Sethi

1997

Mol Gen Genet

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We used a genetic assay to monitor the behavior of sister chromatids during the cell cycle. We show that the ability to induce sister chromatid exchanges (SCE) with ionizing radiation is maximal in budded cells with undivided nuclei and then decreases prior to nuclear division. SCE can be induced in cells arrested in G2 using either nocodazole or cdc mutants. These data show that sister chromatids have two different states prior to nuclear division. We suggest that the sister chromatids of cir. III, a circular derivative of chromosome III, separate (anaphase A) prior to spindle elongation (anaphase B). Other interpretations are also discussed. SCE can be induced in cdc mutants that arrest in G2 and in nocodazole-treated cells, suggesting that mitotic checkpoints arrest cells prior to sister chromatid separation.

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NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae

Cited by 21 publications

References 30 publications

Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3-D modeling of mitotic spindles

Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3-D modeling of mitotic spindles

Genetic evidence for a role of phospholipase C at the budding yeast kinetochore

A change in sister chromatid behavior precedes nuclear division in Saccharomyces cerevisiae

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