ncRAN, a Newly Identified Long Noncoding RNA, Enhances Human Bladder Tumor Growth, Invasion, and Survival

Zhu, Yuyan; Yu, Meng; Li, Zhenhua; Kong, Chuize; Bi, Jianbin; Li, Jun; Gao, Ze-Shou; Li, Zeliang

doi:10.1016/j.urology.2010.09.022

Cited by 75 publications

(68 citation statements)

References 8 publications

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“…Consistent with this view, knockdown of a group of lincRNAs in mouse embryonic stem cells disrupted pluripotency and/or altered expression levels of differentiation markers (Guttman et al, 2011). In addition, genetic mutations of human lincRNAs have been associated with diseases and pathophysiological conditions (Gupta et al, 2010;Hu et al, 2011;Zhu et al, 2011;Cabianca et al, 2012).…”

Section: Introductionmentioning

confidence: 75%

Genome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in Arabidopsis

et al. 2012

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Long intergenic noncoding RNAs (lincRNAs) transcribed from intergenic regions of yeast and animal genomes play important roles in key biological processes. Yet, plant lincRNAs remain poorly characterized and how lincRNA biogenesis is regulated is unclear. Using a reproducibility-based bioinformatics strategy to analyze 200 Arabidopsis thaliana transcriptome data sets, we identified 13,230 intergenic transcripts of which 6480 can be classified as lincRNAs. Expression of 2708 lincRNAs was detected by RNA sequencing experiments. Transcriptome profiling by custom microarrays revealed that the majority of these lincRNAs are expressed at a level between those of mRNAs and precursors of miRNAs. A subset of lincRNA genes shows organ-specific expression, whereas others are responsive to biotic and/or abiotic stresses. Further analysis of transcriptome data in 11 mutants uncovered SERRATE, CAP BINDING PROTEIN20 (CBP20), and CBP80 as regulators of lincRNA expression and biogenesis. RT-PCR experiments confirmed these three proteins are also needed for splicing of a small group of introncontaining lincRNAs.

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Section: Introductionmentioning

confidence: 75%

Genome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in Arabidopsis

et al. 2012

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“…Recently, expression of ncRAN was found to be upregulated in bladder cancers compared with normal tissue. Overexpression of ncRAN in a superficial low-ncRAN expressing bladder cancer cell line enhanced cell proliferation and invasion, whereas shRNA knockdown of ncRAN in a highncRAN expressing invasive bladder cancer cell line sensitized the cells to chemotherapeutic drugs (89). According to these data, ncRAN appears to have oncogenic properties in several cancers.…”

Section: N-myc Alters Expression Of Other Noncoding Rnasmentioning

confidence: 89%

N-myc and Noncoding RNAs in Neuroblastoma

Buechner

Einvik

2012

Molecular Cancer Research

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Neuroblastoma is a pediatric tumor of the sympathetic nervous system. Amplification and overexpression of the MYCN proto-oncogene occurs in approximately 20% of neuroblastomas and is associated with advanced stage disease, rapid tumor progression, and poor prognosis. MYCN encodes the transcriptional regulator N-myc, which has been shown to both up-and downregulate many target genes involved in cell cycle, DNA damage, differentiation, and apoptosis in neuroblastoma. During the last years, it has become clear that N-myc also modulates the expression of several classes of noncoding RNAs, in particular microRNAs. MicroRNAs are the most widely studied noncoding RNA molecules in neuroblastoma. They function as negative regulators of gene expression at the posttranscriptional level in diverse cellular processes. Aberrant regulation of miRNA expression has been implicated in the pathogenesis of neuroblastoma. While the N-myc protein is established as an important regulator of several miRNAs involved in neuroblastoma tumorigenesis, tumor suppressor miRNAs have also been documented to repress MYCN expression and inhibit cell proliferation of MYCN-amplified neuroblastoma cells.

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“…Such therapies would be useful in cases where drugs designed to target proteins have failed, or even in conjunction with available drugs to enhance their effects. 82 However, RNA therapeutics face considerable hurdles, including development of reliable delivery systems, dosage regimes and techniques to ameliorate off-target effects. 113,114 In addition, targeting transcripts the size of lncRNAs may seem like a daunting task; one must screen more small-interfering RNAs compared with mRNAs, possibly because of the extensive secondary structures in lncRNAs.…”

Section: Discussionmentioning

confidence: 99%

“…Identification of lncRNAs correlated to cancer has benefited from the development of a number of effective highthroughput expression analyses technologies as well as from the increasing realization that lncRNAs are emerging as new factors, demonstrating their potential roles in both oncogenic and tumor suppressive pathways. Dozens of lncRNAs have been found to be dysregulated in prostate cancer, breast cancer, colorectal cancer, hepatocellular carcinoma, bladder cancer, lung cancer and others types of cancer (Table 2), [68][69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84] raising the possibility that lncRNAs may become a promising biomarker in cancer diagnosis and prognosis (Figure 2a). For example, increased expression of lncRNA HOTAIR was shown to be associated with metastasis in breast cancer patients, having a unique association with patient prognosis.…”

Section: Lncrnas As Diagnostic and Therapeutic Toolsmentioning

confidence: 99%

The long non-coding RNAs, a new cancer diagnostic and therapeutic gold mine

2013

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The conventional view of gene regulation in biology has centered around protein-coding genes via the central dogma of DNA-mRNA-protein. The discovery of thousands of long non-coding RNAs (lncRNAs) has certainly changed our view of the complexity of mammalian genomes and transcriptomes, as well as many other aspects of biology including transcriptional and posttranscriptional regulation of gene expression. Accumulating reports of misregulated lncRNA expression across numerous cancer types suggest that aberrant lncRNA expression may be a major contributor to tumorigenesis. Here, we summarize recent data about the biological characteristics of lncRNAs in cancer pathways. These include examples with a wide range of molecular mechanisms involved in gene regulation. We also consider the medical implications, and discuss how lncRNAs can be used for cancer diagnosis and prognosis, and serve as potential therapeutic targets. As more examples of regulation by lncRNA are uncovered, one might predict that the large transcripts will eventually rival small RNAs and proteins in their versatility as regulators of genetic information.

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ncRAN, a Newly Identified Long Noncoding RNA, Enhances Human Bladder Tumor Growth, Invasion, and Survival

Cited by 75 publications

References 8 publications

Genome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in Arabidopsis

Genome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in Arabidopsis

N-myc and Noncoding RNAs in Neuroblastoma

The long non-coding RNAs, a new cancer diagnostic and therapeutic gold mine

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